Endosomes generate localized Rho-ROCK-MLC2-based contractile signals via Endo 180 to promote adhesion disassembly

Sturge, Justin; Wienke, Dirk; Isacke, Clare M.

Abstract

The regulated assembly and disassembly of focal adhesions and adherens junctions contributes to cell motility and tumor invasion. Pivotal in this process is phosphorylation of myosin light chain-2 (MLC2) by Rho kinase (ROCK) downstream of Rho activation, which generates the contractile force necessary to drive disassembly of epithelial cell–cell junctions and cell–matrix adhesions at the rear of migrating cells. How Rho–ROCK–MLC2 activation occurs at these distinct cellular locations is not known, but the emerging concept that endocytic dynamics can coordinate key intracellular signaling events provides vital clues. We report that endosomes containing the promigratory receptor Endo180 (CD280) can generate Rho–ROCK–MLC2–based contractile signals. Moreover, we provide evidence for a cellular mechanism in which Endo180-containing endosomes are spatially localized to facilitate their contractile signals directly at sites of adhesion turnover. We propose migration driven by Endo180 as a model for the spatial regulation of contractility and adhesion dynamics by endosomes.

Citation

Sturge, J., Wienke, D., & Isacke, C. M. (2006). Endosomes generate localized Rho-ROCK-MLC2-based contractile signals via Endo 180 to promote adhesion disassembly. Journal of Cell Biology, 175(2), 337-347. https://doi.org/10.1083/jcb.200602125

• Journal Article Type: Article
• Acceptance Date: Sep 11, 2006
• Online Publication Date: Oct 16, 2006
• Publication Date: Oct 23, 2006
• Deposit Date: Jun 27, 2019
• Publicly Available Date: Jul 3, 2019
• Journal: Journal of Cell Biology
• Print ISSN: 0021-9525
• Electronic ISSN: 1540-8140
• Publisher: Rockefeller University Press
• Peer Reviewed: Peer Reviewed
• Volume: 175
• Issue: 2
• Pages: 337-347
• DOI: https://doi.org/10.1083/jcb.200602125
• Keywords: Cell Biology
• Public URL: https://hull-repository.worktribe.com/output/2005814
• Publisher URL: http://jcb.rupress.org/content/175/2/337