A targeted deletion in the endocytic receptor gene Endo 180 results in a defect in collagen uptake

East, Lucy; McCarthy, Afshan; Wienke, Dirk; Sturge, Justin; Ashworth, Alan; Isacke, Clare M.

Abstract

The four members of the mannose receptor family (the mannose receptor, the M-type phospholipase A2 receptor, DEC-205 and Endo180) share a common extracellular arrangement of an amino-terminal cysteine-rich domain followed by a fibronectin type II (FNII) domain and multiple C-type lectin-like domains (CTLDs). In addition, all have a short cytoplasmic domain, which mediates their constitutive recycling between the plasma membrane and the endosomal apparatus, suggesting that these receptors function to internalize ligands for intracellular delivery. We have generated mice with a targeted deletion of Endo180 exons 2-6 and show that this mutation results in the efficient expression of a truncated Endo180 protein that lacks the cysteine-rich domain, the FNII domain and CTLD1. Analysis of embryonic fibroblasts reveals that this mutation does not disrupt the C-type lectin activity that is mediated by CTLD2, but results in cells that have a defect in collagen binding and internalization and an impaired migratory phenotype.

Citation

East, L., McCarthy, A., Wienke, D., Sturge, J., Ashworth, A., & Isacke, C. M. (2003). A targeted deletion in the endocytic receptor gene Endo 180 results in a defect in collagen uptake. EMBO Reports, 4(7), 710-716. https://doi.org/10.1038/sj.embor.embor882

• Journal Article Type: Article
• Acceptance Date: May 13, 2003
• Online Publication Date: Jun 13, 2003
• Publication Date: Jul 1, 2003
• Deposit Date: Jun 27, 2019
• Journal: EMBO Reports
• Print ISSN: 1469-221X
• Electronic ISSN: 1469-3178
• Publisher: EMBO Press
• Peer Reviewed: Peer Reviewed
• Volume: 4
• Issue: 7
• Pages: 710-716
• DOI: https://doi.org/10.1038/sj.embor.embor882
• Public URL: https://hull-repository.worktribe.com/output/2005844
• Publisher URL: https://www.embopress.org/cgi/doi/10.1038/sj.embor.embor882
• Related Public URLs: https://core.ac.uk/reader/192119950