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Multifaceted characterization and in vitro assessment of polyurethane-based electrospun fibrous composite for bone tissue engineering

Mani, Mohan Prasat; Jiang, Haoli; Mani, Mohan Prasath; Jaganathan, Saravana Kumar


Mohan Prasat Mani

Haoli Jiang

Mohan Prasath Mani

Saravana Kumar Jaganathan


Introduction: Recently several new approaches were emerging in bone tissue engineering to develop a substitute for remodelling the damaged tissue. In order to resemble the native extracellular matrix (ECM) of the human tissue, the bone scaffolds must possess necessary requirements like large surface area, interconnected pores and sufficient mechanical strength.
Materials and methods: A novel bone scaffold has been developed using polyurethane (PE) added with wintergreen (WG) and titanium dioxide (TiO2). The developed nanocomposites were characterized through field emission scanning electron microscopy (FESEM), Fourier transform and infrared spectroscopy (FTIR), X-ray diffraction (XRD), contact angle measurement, thermogravimetric analysis (TGA), atomic force microscopy (AFM) and tensile testing. Furthermore, anticoagulant assays, cell viability analysis and calcium deposition were used to investigate the biological properties of the prepared hybrid nanocomposites.
Results: FESEM depicted the reduced fibre diameter for the electrospun PE/WG and PE/WG/TiO2 than the pristine PE. The addition of WG and TiO2 resulted in the alteration in peak intensity of PE as revealed in the FTIR. Wettability measurements showed the PE/WG showed decreased wettability and the PE/WG/TiO2 exhibited improved wettability than the pristine PE. TGA measurements showed the improved thermal behaviour for the PE with the addition of WG and TiO2. Surface analysis indicated that the composite has a smoother surface rather than the pristine PE. Further, the incorporation of WG and TiO2 improved the anticoagulant nature of the pristine PE. In vitro cytotoxicity assay has been performed using fibroblast cells which revealed that the electrospun composites showed good cell attachment and proliferation after 5 days. Moreover, the bone apatite formation study revealed the enhanced deposition of calcium content in the fabricated composites than the pristine PE.
Conclusion: Fabricated nanocomposites rendered improved physico-chemical properties, biocompatibility and calcium deposition which are conducive for bone tissue engineering.


Mani, M. P., Jiang, H., Mani, M. P., & Jaganathan, S. K. (2019). Multifaceted characterization and in vitro assessment of polyurethane-based electrospun fibrous composite for bone tissue engineering. International Journal of Nanomedicine, 14, 8149-8159.

Journal Article Type Article
Acceptance Date Aug 28, 2019
Online Publication Date Oct 8, 2019
Publication Date 2019-10
Deposit Date Nov 20, 2019
Publicly Available Date Nov 21, 2019
Journal International Journal of Nanomedicine
Print ISSN 1178-2013
Electronic ISSN 1178-2013
Publisher Dove Medical Press
Peer Reviewed Peer Reviewed
Volume 14
Pages 8149-8159
Keywords Polymer; TiO2/wintergreen; Surface properties; Apatite formation; Tissue engineering
Public URL
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Copyright Statement
© 2019 Jiang et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at<br /> and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License ( By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For<br /> permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (

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