In the recent decade, the growth of medical textiles is enormous and it paves the sustained development in the quality of life. In this research, an electrospun textile scaffold comprising polyurethane impregnated with Alternanthera sessilis oil was developed for the bone tissue engineering. Morphology analysis showed that the addition of Alternanthera sessilis oil in the polyurethane membrane resulted in reduced fiber diameter (821 ± 140.87 nm) compared to the pristine PU (890 ± 119.11 nm). The presence of Alternanthera sessilis oil in polyurethane membrane was confirmed through hydrogen bond formation as revealed in the infrared analysis. Further, the polyurethane blended with Alternanthera sessilis oil showed hydrophobic (113.7° ± 3.215) and improved surface roughness (329 nm) than the pristine polyurethane (contact angle – 100° ± 0.5774 and Ra – 313 nm). Moreover, the blood compatibility assessments revealed that the developed biocomponent polyurethane/Alternanthera sessilis oil membrane possesses enhanced anticoagulant nature compared to the pristine polyurethane. Finally, the newly developed biocomponent polyurethane/Alternanthera sessilis oil membrane with reduced fiber diameter, hydrophobic behavior, improved surface roughness, and enhanced blood compatibility enabled them as a potential candidate for bone tissue regeneration.