An electrodeposited Au nanoparticle/porous graphene nanoribbon composite for electrochemical detection of alpha-fetoprotein

Jothi, Lavanya; Jaganathan, Saravana Kumar; Nageswaran, Gomathi

Abstract

In this work, a novel label-free electrochemical immunosensor for the detection of alpha-fetoprotein (AFP) is fabricated for early diagnosis and prognostics of liver cancer. A porous graphene nanoribbon (PGNR) was synthesized via chemical reduction method and it was electrodeposited with gold nanoparticles (AuNPs) to obtain AuNPs/PGNR hybrid nanomaterial. Anti-AFP was immobilized onto AuNPs/PGNR/glassy carbon electrode and anti-AFP/AuNPs/PGNR/GCE was further studied to demonstrate its electrocatalytic activity towards AFP antigen. PGNR enhances the electroactive surface area and the electron transfer ability between the electrode and redox probe while the AuNPs deposited on PGNR are used to immobilize biomolecules and to facilitate the electron transport. The superior biosensing performance towards AFP under physiological pH condition is demonstrated by a corresponding decreased peak current in differential pulse voltammetry for a wide linear range (5–60 ng/mL) with a low detection limit of 1 ng/mL. Detection of AFP in serum samples by this label-free electrochemical immunosensor without fouling or significant interference implies that the anti-AFP/AuNPs/PGNR modified GCE has a great application potential for clinical diagnosis of AFP.

Citation

Jothi, L., Jaganathan, S. K., & Nageswaran, G. (2020). An electrodeposited Au nanoparticle/porous graphene nanoribbon composite for electrochemical detection of alpha-fetoprotein. Materials Chemistry and Physics, 242, Article 122514. https://doi.org/10.1016/j.matchemphys.2019.122514

• Journal Article Type: Article
• Acceptance Date: Nov 30, 2019
• Online Publication Date: Dec 2, 2019
• Publication Date: Feb 15, 2020
• Deposit Date: Feb 24, 2020
• Publicly Available Date: Dec 3, 2020
• Journal: Materials Chemistry and Physics
• Print ISSN: 0254-0584
• Electronic ISSN: 0254-0584
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 242
• Article Number: 122514
• DOI: https://doi.org/10.1016/j.matchemphys.2019.122514
• Keywords: Porous graphene nanoribbons; Gold nanoparticles; Alpha fetoprotein; Differential pulse voltammetry
• Public URL: https://hull-repository.worktribe.com/output/3444864
• Publisher URL: https://www.sciencedirect.com/science/article/abs/pii/S0254058419313240?via%3Dihub