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Detection of nitric oxide in tissue samples by ESI-MS

Shen, Zheng; Webster, Abigail; Welham, Kevin J.; Dyer, Charlotte E.; Greenman, John; Haswell, Stephen J.

Authors

Zheng Shen

Abigail Webster

Kevin J. Welham K.J.Welham@hull.ac.uk

Charlotte E. Dyer C.E.Dyer@hull.ac.uk

Stephen J. Haswell

Abstract

A novel method to determine nitric oxide (NO) in biological tissue samples with minimal interference from the cellular detritus is described. Methylpiperazinobenzenediamine, consisting of an o-phenylenediamine and a methyl piperazine group, was chosen as a probe for the detection of NO by mass spectrometry (MS) in biological tissue samples. The o-phenylenediamine group reacts with NO to form a characteristic benzotriazole. The product was identified using electrospray ionization mass spectrometry (ESI-MS) and the method validated within the range of 95-1900 nM. NO levels associated with tissue biopsies (similar to 10 mg) from rat vasculature and intestine tissue biopsies have been successfully determined. The different rates of NO generated from tissue samples under hypoxic and normoxic conditions have been studied by this simple and sensitive method.

Journal Article Type Article
Publication Date Dec 31, 2010
Journal ANALYST
Print ISSN 0003-2654
Electronic ISSN 1364-5528
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 135
Issue 2
Pages 302-305
Institution Citation Shen, Z., Webster, A., Welham, K. J., Dyer, C. E., Greenman, J., & Haswell, S. J. (2010). Detection of nitric oxide in tissue samples by ESI-MS. The Analyst, 135(2), 302-305. doi:10.1039/b917643h
DOI https://doi.org/10.1039/b917643h
Keywords Analytical Chemistry; Spectroscopy; Electrochemistry; Biochemistry; Environmental Chemistry
Publisher URL http://pubs.rsc.org/en/Content/ArticleLanding/2010/AN/B917643H#!divAbstract