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Compensated cardiac hypertrophy is characterised by a decline in palmitate oxidation

Akki, Ashwin; Smith, Katie; Seymour, Anne Marie L.

Authors

Ashwin Akki

Katie Smith

Anne Marie L. Seymour



Abstract

Cardiac hypertrophy is an independent risk factor in the development of heart failure. However, the cellular mechanisms underlying the transition from compensated hypertrophy to heart failure are incompletely understood. The aim of this study was to investigate changes in myocardial substrate utilisation and function in pressure-overload hypertrophy (using C-13 NMR spectroscopy) in parallel with alterations in the expression pattern of genes involved in cardiac fatty acid and glucose uptake and oxidation. Left ventricular hypertrophy was induced surgically in Sprague-Dawley rats by inter-renal aortic constriction. Nine weeks later, hearts were perfused in the isovolumic mode with a physiological mixture of substrates including 5 mM 1-C-13 glucose, 1 mM 3-C-13 lactate, 0.1 mM U-C-13 pyruvate and 0.3 mM U-C-13 palmitate and cardiac function monitored simultaneously. Real-time PCR was used to determine mRNA levels of PPAR alpha and PPAR alpha-regulated metabolic enzymes. Results showed that at the stage of compensated hypertrophy, fatty acid oxidation (FAO) and expression of genes involved in FAO were markedly reduced, whilst pyruvate oxidation was enhanced, highlighting the fact that metabolic remodelling is an early event in the development of cardiac hypertrophy.

Citation

Akki, A., Smith, K., & Seymour, A. M. L. (2008). Compensated cardiac hypertrophy is characterised by a decline in palmitate oxidation. Molecular and Cellular Biochemistry, 311(1-2), 215-224. doi:10.1007/s11010-008-9711-y

Journal Article Type Article
Acceptance Date Apr 30, 2008
Online Publication Date Feb 16, 2008
Publication Date Apr 1, 2008
Journal MOLECULAR AND CELLULAR BIOCHEMISTRY
Print ISSN 0300-8177
Electronic ISSN 1573-4919
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 311
Issue 1-2
Pages 215-224
DOI https://doi.org/10.1007/s11010-008-9711-y
Keywords Clinical Biochemistry; Cell Biology; Molecular Biology; General Medicine
Public URL https://hull-repository.worktribe.com/output/395955