Abigail Webster
A microfluidic device for tissue biopsy culture and interrogation
Webster, Abigail; Dyer, Charlotte E.; Haswell, Stephen J.; Greenman, John
Authors
Dr Charlotte Dyer C.E.Dyer@hull.ac.uk
Lecturer in Biomedical Sciences
Stephen J. Haswell
Professor John Greenman J.Greenman@hull.ac.uk
Professor of Tumour Immunology
Abstract
This communication reports the development of a microfluidic device capable of maintaining the long-term culture of viable tissue biopsies. Tissue-based models will enable evaluation of cell-cell and cell-matrix interactions within multi-cellular systems. The device demonstrated is a prototype, fabricated with the capacity to receive biopsy samples up to 2 mm(3), from various tissue sources. Presently, this system has been tested with human colorectal tissue biopsies, for periods in excess of 3 days. The response of normal colorectal tissue and neoplastic biopsies to hypoxia was assayed by the release of vascular endothelial growth factor (VEGF) into the media, which was measured off-chip. As anticipated, the hypoxia induced a greater VEGF response in the tumour biopsies than the nonmalignant tissue.
Citation
Webster, A., Dyer, C. E., Haswell, S. J., & Greenman, J. (2010). A microfluidic device for tissue biopsy culture and interrogation. Analytical Methods, 2(8), 1005-1007. https://doi.org/10.1039/c0ay00293c
Journal Article Type | Article |
---|---|
Acceptance Date | Jul 5, 2010 |
Publication Date | Aug 1, 2010 |
Journal | ANALYTICAL METHODS |
Print ISSN | 1759-9660 |
Publisher | Royal Society of Chemistry |
Peer Reviewed | Peer Reviewed |
Volume | 2 |
Issue | 8 |
Pages | 1005-1007 |
DOI | https://doi.org/10.1039/c0ay00293c |
Keywords | endothelial growth-factor microenvironment microfabrication expression perfusion chamber |
Public URL | https://hull-repository.worktribe.com/output/396316 |
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