A microfluidic device for tissue biopsy culture and interrogation

Webster, Abigail; Dyer, Charlotte E.; Haswell, Stephen J.; Greenman, John

Abstract

This communication reports the development of a microfluidic device capable of maintaining the long-term culture of viable tissue biopsies. Tissue-based models will enable evaluation of cell-cell and cell-matrix interactions within multi-cellular systems. The device demonstrated is a prototype, fabricated with the capacity to receive biopsy samples up to 2 mm(3), from various tissue sources. Presently, this system has been tested with human colorectal tissue biopsies, for periods in excess of 3 days. The response of normal colorectal tissue and neoplastic biopsies to hypoxia was assayed by the release of vascular endothelial growth factor (VEGF) into the media, which was measured off-chip. As anticipated, the hypoxia induced a greater VEGF response in the tumour biopsies than the nonmalignant tissue.

Citation

Webster, A., Dyer, C. E., Haswell, S. J., & Greenman, J. (2010). A microfluidic device for tissue biopsy culture and interrogation. Analytical Methods, 2(8), 1005-1007. https://doi.org/10.1039/c0ay00293c

• Journal Article Type: Article
• Acceptance Date: Jul 5, 2010
• Publication Date: Aug 1, 2010
• Journal: ANALYTICAL METHODS
• Print ISSN: 1759-9660
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 2
• Issue: 8
• Pages: 1005-1007
• DOI: https://doi.org/10.1039/c0ay00293c
• Keywords: endothelial growth-factor microenvironment microfabrication expression perfusion chamber
• Public URL: https://hull-repository.worktribe.com/output/396316