A microfluidic device for tissue biopsy culture and interrogation
Webster, Abigail; Dyer, Charlotte E.; Haswell, Stephen J.; Greenman, John
Charlotte E. Dyer C.E.Dyer@hull.ac.uk
Stephen J. Haswell
Professor John Greenman J.Greenman@hull.ac.uk
Professor of Tumour Immunology
This communication reports the development of a microfluidic device capable of maintaining the long-term culture of viable tissue biopsies. Tissue-based models will enable evaluation of cell-cell and cell-matrix interactions within multi-cellular systems. The device demonstrated is a prototype, fabricated with the capacity to receive biopsy samples up to 2 mm(3), from various tissue sources. Presently, this system has been tested with human colorectal tissue biopsies, for periods in excess of 3 days. The response of normal colorectal tissue and neoplastic biopsies to hypoxia was assayed by the release of vascular endothelial growth factor (VEGF) into the media, which was measured off-chip. As anticipated, the hypoxia induced a greater VEGF response in the tumour biopsies than the nonmalignant tissue.
Webster, A., Dyer, C. E., Haswell, S. J., & Greenman, J. (2010). A microfluidic device for tissue biopsy culture and interrogation. Analytical Methods, 2(8), 1005-1007. doi:10.1039/c0ay00293c
|Journal Article Type||Article|
|Acceptance Date||Jul 5, 2010|
|Publication Date||Aug 1, 2010|
|Publisher||Royal Society of Chemistry|
|Peer Reviewed||Peer Reviewed|
|Keywords||endothelial growth-factor microenvironment microfabrication expression perfusion chamber|
This file is under embargo due to copyright reasons.
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