Samantha M. Hattersley
A microfluidic system for testing the responses of head and neck squamous cell carcinoma tissue biopsies to treatment with chemotherapy drugs
Hattersley, Samantha M.; Sylvester, Deborah C.; Dyer, Charlotte E.; Stafford, Nicholas D.; Haswell, Stephen J.; Greenman, John
Deborah C. Sylvester
Charlotte E. Dyer C.E.Dyer@hull.ac.uk
Nicholas D. Stafford
Stephen J. Haswell
Professor John Greenman J.Greenman@hull.ac.uk
Professor of Tumour Immunology
Tumors are heterogeneous masses of cells characterized pathologically by their size and spread. Their chaotic biology makes treatment of malignancies hard to generalize. We present a robust and reproducible glass microfluidic system, for the maintenance and ‘‘interrogation’’ of head and neck squamous cell carcinoma (HNSCC) tumor biopsies, which enables continuous media perfusion and waste removal, recreating conditions. Primary HNSCC or metastatic lymph samples were subsequently treated with 5-fluorouracil and cisplatin, alone and in combination, and were monitored for viability and apoptotic biomarker release ‘off-chip’ over 7 days. The concentration of lactate dehydrogenase was initially high but rapidly dropped to minimally detectable levels in all tumor samples; conversely, effluent concentration of WST-1 (cell proliferation) increased over 7 days: both factors demonstrating cell viability. Addition of cell lysis reagent resulted in increased cell death and reduction in cell proliferation. An apoptotic biomarker, cytochrome treated samples showed higher levels than the control, with the combination therapy showing the greatest effect. Hematoxylin- and Eosin-stained sections from the biopsy, before and after maintenance, demonstrated the preservation of tissue architecture. This device offers a novel method of studying the tumor environment, and offers a pre-clinical model for creating personalized treatment regimens.
Hattersley, S. M., Sylvester, D. C., Dyer, C. E., Stafford, N. D., Haswell, S. J., & Greenman, J. (2012). A microfluidic system for testing the responses of head and neck squamous cell carcinoma tissue biopsies to treatment with chemotherapy drugs. Annals of biomedical engineering, 40(6), 1277-1288. doi:10.1007/s10439-011-0428-9
|Journal Article Type||Article|
|Acceptance Date||Oct 1, 2011|
|Online Publication Date||Oct 15, 2011|
|Journal||ANNALS OF BIOMEDICAL ENGINEERING|
|Peer Reviewed||Peer Reviewed|
|Keywords||Head and neck squamous cell carcinoma; 5-fluorouracil; Cisplatin; Lactate dehydrogenase; Cytochrome c|
This file is under embargo due to copyright reasons.
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