Improved DNA extraction efficiency from low level cell numbers using a silica monolith based micro fluidic device

Kashkary, Loay; Kemp, Cordula; Shaw, Kirsty J.; Greenway, Gillian M.; Haswell, Stephen J.

Abstract

The evaluation of a micro fluidic system with an integrated silica monolith for performing DNA extraction from limited biological samples has been carried out. Low DNA target concentrations usually require the addition of carrier RNA to ensure desired extraction efficiencies. Here, we demonstrate a micro fluidic extraction system with increasingly efficient extraction performances for biological samples containing <15ng of total DNA without the need of adding carrier nucleic acids. All extracted DNA showed successful amplification via the polymerase chain reaction demonstrating both the effectiveness of the proposed system at removing potential inhibitors and yielding good quality DNA. The work presented here beneficially identifies reduced sample volumes/concentrations as suitable for processing with respect to downstream analysis by enabling pre-concentration of the biological sample, particularly important when dealing with clinical or forensic specimens.

Citation

Kashkary, L., Kemp, C., Shaw, K. J., Greenway, G. M., & Haswell, S. J. (2012). Improved DNA extraction efficiency from low level cell numbers using a silica monolith based micro fluidic device. Analytica Chimica Acta, 750, 127-131. https://doi.org/10.1016/j.aca.2012.05.019

• Journal Article Type: Article
• Acceptance Date: May 12, 2012
• Online Publication Date: May 21, 2012
• Publication Date: 2012-10
• Journal: Analytica Chimica Acta
• Print ISSN: 0003-2670
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 750
• Pages: 127-131
• DOI: https://doi.org/10.1016/j.aca.2012.05.019
• Keywords: DNA extraction; Monolith; Micro fluidic
• Public URL: https://hull-repository.worktribe.com/output/424111