Dr Simon Calaminus S.Calaminus@hull.ac.uk
Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny
Calaminus, Simon D J; Guitart, Amelie; Sinclair, Amy; Schachtner, Hannah; Watson, Steve P.; Holyoake, Tessa L.; Kranc, Kamil R.; Machesky, Laura M.
Authors
Amelie Guitart
Amy Sinclair
Hannah Schachtner
Steve P. Watson
Tessa L. Holyoake
Kamil R. Kranc
Laura M. Machesky
Contributors
Kathleen Freson
Editor
Abstract
The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. Consequently, we demonstrate that Pf4-Cre activity is not megakaryocyte lineage-specific but extends to other myeloid and lymphoid lineages at significant levels between 15-60%. Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage. © 2012 Calaminus et al.
Citation
Calaminus, S. D. J., Guitart, A., Sinclair, A., Schachtner, H., Watson, S. P., Holyoake, T. L., …Machesky, L. M. (2012). Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. PLoS ONE, 7(12), e51361. https://doi.org/10.1371/journal.pone.0051361
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 7, 2012 |
Online Publication Date | Dec 27, 2012 |
Publication Date | Dec 27, 2012 |
Publicly Available Date | Mar 28, 2024 |
Print ISSN | 1932-6203 |
Electronic ISSN | 1932-6203 |
Publisher | Public Library of Science |
Peer Reviewed | Peer Reviewed |
Volume | 7 |
Issue | 12 |
Article Number | e51361 |
Pages | e51361 |
DOI | https://doi.org/10.1371/journal.pone.0051361 |
Keywords | General Biochemistry, Genetics and Molecular Biology; General Agricultural and Biological Sciences; General Medicine |
Public URL | https://hull-repository.worktribe.com/output/429016 |
Publisher URL | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0051361 |
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Copyright Statement
© 2012 Calaminus et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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