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Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny

Calaminus, Simon D J; Guitart, Amelie; Sinclair, Amy; Schachtner, Hannah; Watson, Steve P.; Holyoake, Tessa L.; Kranc, Kamil R.; Machesky, Laura M.

Authors

Amelie Guitart

Amy Sinclair

Hannah Schachtner

Steve P. Watson

Tessa L. Holyoake

Kamil R. Kranc

Laura M. Machesky



Contributors

Kathleen Freson
Editor

Abstract

The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. Consequently, we demonstrate that Pf4-Cre activity is not megakaryocyte lineage-specific but extends to other myeloid and lymphoid lineages at significant levels between 15-60%. Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage. © 2012 Calaminus et al.

Citation

Calaminus, S. D. J., Guitart, A., Sinclair, A., Schachtner, H., Watson, S. P., Holyoake, T. L., …Machesky, L. M. (2012). Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. PLoS ONE, 7(12), e51361. https://doi.org/10.1371/journal.pone.0051361

Journal Article Type Article
Acceptance Date Nov 7, 2012
Online Publication Date Dec 27, 2012
Publication Date Dec 27, 2012
Publicly Available Date Mar 28, 2024
Print ISSN 1932-6203
Electronic ISSN 1932-6203
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 7
Issue 12
Article Number e51361
Pages e51361
DOI https://doi.org/10.1371/journal.pone.0051361
Keywords General Biochemistry, Genetics and Molecular Biology; General Agricultural and Biological Sciences; General Medicine
Public URL https://hull-repository.worktribe.com/output/429016
Publisher URL https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0051361

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Copyright Statement
© 2012 Calaminus et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.





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