Simon D J Calaminus
Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny
Calaminus, Simon D J; Guitart, Amelie; Sinclair, Amy; Schachtner, Hannah; Watson, Steve P.; Holyoake, Tessa L.; Kranc, Kamil R.; Machesky, Laura M.
Steve P. Watson
Tessa L. Holyoake
Kamil R. Kranc
Laura M. Machesky
The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. Consequently, we demonstrate that Pf4-Cre activity is not megakaryocyte lineage-specific but extends to other myeloid and lymphoid lineages at significant levels between 15-60%. Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage. © 2012 Calaminus et al.
Calaminus, S. D. J., Guitart, A., Sinclair, A., Schachtner, H., Watson, S. P., Holyoake, T. L., …Machesky, L. M. (2012). Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. PLoS ONE, 7(12), e51361. https://doi.org/10.1371/journal.pone.0051361
|Journal Article Type||Article|
|Acceptance Date||Nov 7, 2012|
|Online Publication Date||Dec 27, 2012|
|Publication Date||Dec 27, 2012|
|Publicly Available Date||Oct 27, 2022|
|Publisher||Public Library of Science|
|Peer Reviewed||Peer Reviewed|
|Keywords||General Biochemistry, Genetics and Molecular Biology; General Agricultural and Biological Sciences; General Medicine|
Publisher Licence URL
© 2012 Calaminus et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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