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Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny

Calaminus, Simon D J; Guitart, Amelie; Sinclair, Amy; Schachtner, Hannah; Watson, Steve P.; Holyoake, Tessa L.; Kranc, Kamil R.; Machesky, Laura M.

Authors

Simon D J Calaminus

Amelie Guitart

Amy Sinclair

Hannah Schachtner

Steve P. Watson

Tessa L. Holyoake

Kamil R. Kranc

Laura M. Machesky



Contributors

Kathleen Freson
Editor

Abstract

The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. Consequently, we demonstrate that Pf4-Cre activity is not megakaryocyte lineage-specific but extends to other myeloid and lymphoid lineages at significant levels between 15-60%. Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage. © 2012 Calaminus et al.

Journal Article Type Article
Publication Date Dec 27, 2012
Journal PLoS ONE
Print ISSN 1932-6203
Electronic ISSN 1932-6203
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 7
Issue 12
Article Number e51361
Pages e51361
APA6 Citation Calaminus, S. D. J., Guitart, A., Sinclair, A., Schachtner, H., Watson, S. P., Holyoake, T. L., …Machesky, L. M. (2012). Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. PloS one, 7(12), e51361. https://doi.org/10.1371/journal.pone.0051361
DOI https://doi.org/10.1371/journal.pone.0051361
Keywords General Biochemistry, Genetics and Molecular Biology; General Agricultural and Biological Sciences; General Medicine
Publisher URL https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0051361

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Copyright Statement
© 2012 Calaminus et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



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