Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny

Calaminus, Simon D J; Guitart, Amelie; Sinclair, Amy; Schachtner, Hannah; Watson, Steve P.; Holyoake, Tessa L.; Kranc, Kamil R.; Machesky, Laura M.

Abstract

The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. Consequently, we demonstrate that Pf4-Cre activity is not megakaryocyte lineage-specific but extends to other myeloid and lymphoid lineages at significant levels between 15-60%. Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage. © 2012 Calaminus et al.

Citation

Calaminus, S. D. J., Guitart, A., Sinclair, A., Schachtner, H., Watson, S. P., Holyoake, T. L., Kranc, K. R., & Machesky, L. M. (2012). Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. PLoS ONE, 7(12), e51361. https://doi.org/10.1371/journal.pone.0051361

• Journal Article Type: Article
• Acceptance Date: Nov 7, 2012
• Online Publication Date: Dec 27, 2012
• Publication Date: Dec 27, 2012
• Publicly Available Date: Aug 6, 2019
• Print ISSN: 1932-6203
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 7
• Issue: 12
• Article Number: e51361
• Pages: e51361
• DOI: https://doi.org/10.1371/journal.pone.0051361
• Keywords: General Biochemistry, Genetics and Molecular Biology; General Agricultural and Biological Sciences; General Medicine
• Public URL: https://hull-repository.worktribe.com/output/429016
• Publisher URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0051361