Dr Barbara Guinn B.Guinn@hull.ac.uk
Reader in Biomedical Sciences
A Combination of the Immunotherapeutic Drug Anti-Programmed Death 1 with Lenalidomide Enhances Specific T Cell Immune Responses against Acute Myeloid Leukemia Cells
Guinn, Barbara Ann; Schuler, Patrick J; Schrezenmeier, Hubert; Hofmann, Susanne; Weiss, Johanna; Bulach, Christiane; Götz, Marlies; Greiner, Jochen
Authors
Patrick J Schuler
Hubert Schrezenmeier
Susanne Hofmann
Johanna Weiss
Christiane Bulach
Marlies Götz
Jochen Greiner
Abstract
Immune checkpoint inhibitors can block inhibitory molecules on the surface of T cells, switching them from an exhausted to an active state. One of these inhibitory immune checkpoints, programmed cell death protein 1 (PD-1) is expressed on T cell subpopulations in acute myeloid leukemia (AML). PD-1 expression has been shown to increase with AML progression following allo-haematopoeitic stem cell transplantation, and therapy with hypomethylating agents. We have previously shown that anti-PD-1 can enhance the response of leukemia-associated antigen (LAA)-specific T cells against AML cells as well as leukemic stem and leukemic progenitor cells (LSC/LPCs) ex vivo. In concurrence, blocking of PD-1 with antibodies such as nivolumab has been shown to enhance response rates post-chemotherapy and stem cell transplant. The immune modulating drug lenalidomide has been shown to promote anti-tumour immunity including anti-inflammatory, anti-proliferative, pro-apoptotic and anti-angiogenicity. The effects of lenalidomide are distinct from chemotherapy, hypomethylating agents or kinase inhibitors, making lenalidomide an attractive agent for use in AML and in combination with existing active agents. To determine whether anti-PD-1 (nivolumab) and lenalidomide alone or in combination could enhance LAA-specific T cell immune responses, we used colony-forming immune and ELISpot assays. Combinations of immunotherapeutic approaches are believed to increase antigen-specific immune responses against leukemic cells including LPC/LSCs. In this study we used a combination of LAA-peptides with the immune checkpoint inhibitor anti-PD-1 and lenalidomide to enhance the killing of LSC/LPCs ex vivo. Our data offer a novel insight into how we could improve AML patient responses to treatment in future clinical studies.
Citation
Guinn, B. A., Schuler, P. J., Schrezenmeier, H., Hofmann, S., Weiss, J., Bulach, C., …Greiner, J. (2023). A Combination of the Immunotherapeutic Drug Anti-Programmed Death 1 with Lenalidomide Enhances Specific T Cell Immune Responses against Acute Myeloid Leukemia Cells. International Journal of Molecular Sciences, 24(11), Article 9285. https://doi.org/10.3390/ijms24119285
Journal Article Type | Article |
---|---|
Acceptance Date | May 23, 2023 |
Online Publication Date | May 26, 2023 |
Publication Date | Jun 1, 2023 |
Deposit Date | May 26, 2023 |
Publicly Available Date | Jun 1, 2023 |
Journal | International journal of molecular sciences |
Electronic ISSN | 1422-0067 |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Volume | 24 |
Issue | 11 |
Article Number | 9285 |
DOI | https://doi.org/10.3390/ijms24119285 |
Keywords | Acute myeloid leukemia; Anti-programmed death 1 (PD-1); Lenalidomide; Immunotherapy; Leukemia-associated antigens |
Public URL | https://hull-repository.worktribe.com/output/4297932 |
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Copyright Statement
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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