Endo180 modulation by bisphosphonates and diagnostic accuracy in metastatic breast cancer

Palmieri, C; Caley, M P; Purshouse, K; Fonseca, A-V; Rodriguez-Teja, M; Kogianni, G; Woodley, L; Odendaal, J; Elliott, K; Waxman, J; Sturge, Justin

Abstract

Background:Endo180 (CD280; MRC2; uPARAP)-dependent collagen remodelling is dysregulated in primary tumours and bone metastasis. Here, we confirm the release and diagnostic accuracy of soluble Endo180 for diagnosing metastasis in breast cancer (BCa).Methods:Endo180 was quantified in BCa cell conditioned medium and plasma from BCa patients stratified according to disease status and bisphosphonate treatment (n=88). All P-values are from two-sided tests.Results:Endo180 is released by ectodomain shedding from the surface of MCF-7 and MDA-MB-231 BCa cell lines. Plasma Endo180 was significantly higher in recurrent/metastatic (1.71±0.87; n=59) vs early/localised (0.92±0.37; n=29) BCa (P<0.0001). True/false-positive rates for metastasis classification were: 85%/50% for the reference standard, CA 15-3 antigen (28 U ml-1); 97%/36% for Endo180; and 97%/32% for CA 15-3 antigen+Endo180. Bisphosphonate treatment was associated with reduced Endo180 levels in BCa patients with bone metastasis (P=0.011; n=42). True/false-positive rates in bisphosphonate-naive patients (n=57) were: 68%/45% for CA 15-3 antigen; 95%/20% for Endo180; and 92%/21% for CA 15-3 antigen+Endo180.Conclusion:Endo180 is a potential marker modulated by bisphosphonates in metastatic BCa.

Citation

Palmieri, C., Caley, M. P., Purshouse, K., Fonseca, A.-V., Rodriguez-Teja, M., Kogianni, G., Woodley, L., Odendaal, J., Elliott, K., Waxman, J., & Sturge, J. (2013). Endo180 modulation by bisphosphonates and diagnostic accuracy in metastatic breast cancer. The British Journal of Cancer, 108(1), 163-169. https://doi.org/10.1038/bjc.2012.540

• Journal Article Type: Article
• Acceptance Date: Dec 31, 2013
• Online Publication Date: Dec 20, 2012
• Publication Date: 2013-01
• Journal: British Journal of Cancer
• Print ISSN: 0007-0920
• Electronic ISSN: 1532-1827
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 108
• Issue: 1
• Pages: 163-169
• DOI: https://doi.org/10.1038/bjc.2012.540
• Keywords: Cancer Research; Oncology
• Public URL: https://hull-repository.worktribe.com/output/432500