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The Rac GEF ZizB regulates development, cell motility and cytokinesis in Dictyostelium

Pakes, Nicholl K.; Veltman, Douwe M.; Rivero, Francisco; Nasir, Jamal; Insall, Robert; Williams, Robin S.B.

Authors

Nicholl K. Pakes

Douwe M. Veltman

Jamal Nasir

Robert Insall

Robin S.B. Williams



Abstract

Dock (dedicator of cytokinesis) proteins represent a family of guanine nucleotide exchange factors (GEFs) that include the well-studied Dock180 family and the poorly characterised zizimin family. Our current understanding of Dock180 function is that it regulates Rho small GTPases and thus has a role in a number of cell processes, including cell migration, development and division. Here, we use a tractable model for cell motility research, Dictyostelium discoideum, to help elucidate the role of the related zizimin proteins. We show that gene ablation of zizA causes no change in development, whereas ablation of zizB gives rise to an aberrant developmental morphology and a reduction in cell directionality and velocity, and altered cell shape. Fluorescently labelled ZizA protein associates with the microtubule-organising centre (MTOC), whereas ZizB is enriched in the cortex. Overexpression of ZizB also causes an increase in the number of filopodia and a partial inhibition of cytokinesis. Analysis of ZizB protein binding partners shows that it interacts with Rac1a and a range of actin-associated proteins. In conclusion, our work provides insight into the molecular and cellular functions of zizimin GEF proteins, which are shown to have a role in cell movement, filopodia formation and cytokinesis. © 2012. Published by The Company of Biologists Ltd.

Citation

Pakes, N. K., Veltman, D. M., Rivero, F., Nasir, J., Insall, R., & Williams, R. S. (2012). The Rac GEF ZizB regulates development, cell motility and cytokinesis in Dictyostelium. Journal of cell science, 125(10), 2457-2465. https://doi.org/10.1242/jcs.100966

Journal Article Type Article
Acceptance Date Jan 25, 2012
Online Publication Date May 15, 2012
Publication Date 2012-05
Deposit Date Apr 1, 2022
Publicly Available Date Apr 12, 2022
Journal Journal of Cell Science
Print ISSN 0021-9533
Publisher The Company of Biologists
Peer Reviewed Peer Reviewed
Volume 125
Issue 10
Pages 2457-2465
DOI https://doi.org/10.1242/jcs.100966
Keywords Chemotaxis; D. discoideum; Dock protein; Rac GEF; Zizimin
Public URL https://hull-repository.worktribe.com/output/540934

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Publisher Licence URL
https://creativecommons.org/licenses/by-nc-sa/3.0/

Copyright Statement
© 2012. Published by The Company of Biologists Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0/), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.





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