Nicholl K. Pakes
The Rac GEF ZizB regulates development, cell motility and cytokinesis in Dictyostelium
Pakes, Nicholl K.; Veltman, Douwe M.; Rivero, Francisco; Nasir, Jamal; Insall, Robert; Williams, Robin S.B.
Authors
Douwe M. Veltman
Dr Francisco Rivero Crespo F.Rivero-Crespo@hull.ac.uk
Reader in Biomedical Science
Jamal Nasir
Robert Insall
Robin S.B. Williams
Abstract
Dock (dedicator of cytokinesis) proteins represent a family of guanine nucleotide exchange factors (GEFs) that include the well-studied Dock180 family and the poorly characterised zizimin family. Our current understanding of Dock180 function is that it regulates Rho small GTPases and thus has a role in a number of cell processes, including cell migration, development and division. Here, we use a tractable model for cell motility research, Dictyostelium discoideum, to help elucidate the role of the related zizimin proteins. We show that gene ablation of zizA causes no change in development, whereas ablation of zizB gives rise to an aberrant developmental morphology and a reduction in cell directionality and velocity, and altered cell shape. Fluorescently labelled ZizA protein associates with the microtubule-organising centre (MTOC), whereas ZizB is enriched in the cortex. Overexpression of ZizB also causes an increase in the number of filopodia and a partial inhibition of cytokinesis. Analysis of ZizB protein binding partners shows that it interacts with Rac1a and a range of actin-associated proteins. In conclusion, our work provides insight into the molecular and cellular functions of zizimin GEF proteins, which are shown to have a role in cell movement, filopodia formation and cytokinesis. © 2012. Published by The Company of Biologists Ltd.
Citation
Pakes, N. K., Veltman, D. M., Rivero, F., Nasir, J., Insall, R., & Williams, R. S. (2012). The Rac GEF ZizB regulates development, cell motility and cytokinesis in Dictyostelium. Journal of cell science, 125(10), 2457-2465. https://doi.org/10.1242/jcs.100966
Journal Article Type | Article |
---|---|
Acceptance Date | Jan 25, 2012 |
Online Publication Date | May 15, 2012 |
Publication Date | 2012-05 |
Deposit Date | Apr 1, 2022 |
Publicly Available Date | Apr 12, 2022 |
Journal | Journal of Cell Science |
Print ISSN | 0021-9533 |
Publisher | The Company of Biologists |
Peer Reviewed | Peer Reviewed |
Volume | 125 |
Issue | 10 |
Pages | 2457-2465 |
DOI | https://doi.org/10.1242/jcs.100966 |
Keywords | Chemotaxis; D. discoideum; Dock protein; Rac GEF; Zizimin |
Public URL | https://hull-repository.worktribe.com/output/540934 |
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Copyright Statement
© 2012. Published by The Company of Biologists Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0/), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.
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