Skip to main content

Research Repository

Advanced Search

Paper-based analytical devices for colorimetric detection of: S. aureus and E. coli and their antibiotic resistant strains in milk

Khan, Qaiser Mahmood; Asif, Muhammad; Awan, Fazli Rabbi; Khan, Qaiser Mahmood; Ngamsom, Bongkot; Pamme, Nicole

Authors

Qaiser Mahmood Khan

Muhammad Asif

Fazli Rabbi Awan

Qaiser Mahmood Khan

Bongkot Ngamsom



Abstract

Animal derived milk which is an important part of human diet due to its high nutritional value not only supports humans but also presents a growth environment for pathogenic bacteria. Milk may become contaminated with bacteria through udder infections or through contact within the dairy farm environment. Infections are treated with antibiotics, with β-lactams most commonly used in veterinary medicine. However, their frequent use leads to the emergence of β-lactam resistant bacterial strains, which causes difficulties in the treatment of infections in both humans and animals. Detection of pathogens as well as their antibiotic sensitivity is a pre-requisite for successful treatment and this is generally achieved with laboratory-based techniques such as growth inhibition assays, enzyme-linked immunosorbent assays (ELISA) or polymerase chain reactions (PCRs), which are unavailable in resource-limited settings. Here, we investigated paper-based analytical devices (μPADs) for the presumptive detection of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) and their antibiotic resistant bacterial strains in milk samples. The μPADs were fabricated on filter paper using wax printing, and then impregnated with chromogenic substrates, which reacted with bacterial enzymes to form coloured products. Limits of detection of S. aureus and E. coli and their antibiotic resistant strains in milk samples were found to be 106 cfu mL-1. Enrichment of milk samples in a selective medium for 12 h enabled detection as low as 10 cfu mL-1. The paper devices tested on a set of 640 milk samples collected from dairy animals in Pakistan demonstrated more than 90% sensitivity and 100% selectivity compared to PCR, showing promise to provide inexpensive and portable diagnostic solutions for the detection of pathogenic bacteria in resource-limited settings.

Citation

Khan, Q. M., Asif, M., Awan, F. R., Khan, Q. . M., Ngamsom, B., & Pamme, N. (2020). Paper-based analytical devices for colorimetric detection of: S. aureus and E. coli and their antibiotic resistant strains in milk. Analyst, 145(22), 7320-7329. https://doi.org/10.1039/d0an01075h

Journal Article Type Article
Acceptance Date Aug 9, 2020
Online Publication Date Aug 10, 2020
Publication Date Nov 21, 2020
Deposit Date Aug 11, 2020
Publicly Available Date Aug 12, 2020
Journal Analyst
Print ISSN 0003-2654
Electronic ISSN 1364-5528
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 145
Issue 22
Pages 7320-7329
DOI https://doi.org/10.1039/d0an01075h
Keywords Analytical Chemistry; Spectroscopy; Electrochemistry; Biochemistry; Environmental Chemistry
Public URL https://hull-repository.worktribe.com/output/3557121
Publisher URL https://pubs.rsc.org/en/Content/ArticleLanding/2020/AN/D0AN01075H#!divAbstract
Additional Information : This document is Similarity Check deposited; : Supplementary Information; : Single-blind; : Received 28 May 2020; Accepted 9 August 2020; Accepted Manuscript published 10 August 2020

Files






You might also like



Downloadable Citations