Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya
Ngamsom, Bongkot; Wandera, Ernest Apondi; Wandera, Ernest Apondi; Iles, Alexander; Kimani, Racheal; Muregi, Francis; Gitaka, Jesse; Pamme, Nicole
Ernest Apondi Wandera
Ernest Apondi Wandera
Dr Alex Iles Alexander.Iles@hull.ac.uk
Experimental Officer - Lab on a Chip
Professor Nicole Pamme N.Pamme@hull.ac.uk
Professor in Analytical Chemistry
© The Royal Society of Chemistry 2019. We report the rapid detection (20 min) of Streptococcus agalactiae, Group B Streptococcus (GBS) employing on-chip magnetic isolation of GBS based on immiscible filtration assisted by surface tension (IFAST), followed by detection of the isolated GBS using an adenosine triphosphate (ATP) bioluminescence assay. Up to 80% GBS cells were isolated from spiked artificial urine samples with linear responses of bioluminescence signals from isolated cells at 2.3 × 102-9.1 × 105 CFU mL-1, demonstrating great promise for point-of-care detection of pathogenic bacteria in screening urine samples from pregnant women. Practical challenges during initial testing of the developed protocol with urine samples in Kenya are also described.
|Journal Article Type||Article|
|Publication Date||Oct 10, 2019|
|Publisher||Royal Society of Chemistry|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Wandera, E. A., Ngamsom, B., Wandera, E. . . A., Iles, A., Kimani, R., Muregi, F., …Pamme, N. (2019). Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya. Analyst, 144(23), 6889-6897. https://doi.org/10.1039/c9an01808e|
|Keywords||Analytical Chemistry; Spectroscopy; Electrochemistry; Biochemistry; Environmental Chemistry|
|Additional Information||: This document is Similarity Check deposited; : Supplementary Information; : Single-blind; : Received 13 September 2019; Accepted 9 October 2019; Accepted Manuscript published 10 October 2019|
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