Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya

Ngamsom, Bongkot; Wandera, Ernest Apondi; Iles, Alexander; Kimani, Racheal; Muregi, Francis; Gitaka, Jesse; Pamme, Nicole

Abstract

© The Royal Society of Chemistry 2019. We report the rapid detection (20 min) of Streptococcus agalactiae, Group B Streptococcus (GBS) employing on-chip magnetic isolation of GBS based on immiscible filtration assisted by surface tension (IFAST), followed by detection of the isolated GBS using an adenosine triphosphate (ATP) bioluminescence assay. Up to 80% GBS cells were isolated from spiked artificial urine samples with linear responses of bioluminescence signals from isolated cells at 2.3 × 102-9.1 × 105 CFU mL-1, demonstrating great promise for point-of-care detection of pathogenic bacteria in screening urine samples from pregnant women. Practical challenges during initial testing of the developed protocol with urine samples in Kenya are also described.

Citation

Ngamsom, B., Wandera, E. A., Iles, A., Kimani, R., Muregi, F., Gitaka, J., & Pamme, N. (2019). Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya. Analyst, 144(23), 6889-6897. https://doi.org/10.1039/c9an01808e

• Journal Article Type: Article
• Acceptance Date: Oct 9, 2019
• Online Publication Date: Oct 10, 2019
• Publication Date: Oct 10, 2019
• Deposit Date: Oct 11, 2019
• Publicly Available Date: Oct 11, 2020
• Journal: Analyst
• Print ISSN: 0003-2654
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 144
• Issue: 23
• Pages: 6889-6897
• DOI: https://doi.org/10.1039/c9an01808e
• Keywords: Analytical Chemistry; Spectroscopy; Electrochemistry; Biochemistry; Environmental Chemistry
• Public URL: https://hull-repository.worktribe.com/output/2909690
• Publisher URL: https://pubs.rsc.org/en/Content/ArticleLanding/2019/AN/C9AN01808E#!divAbstract
• Additional Information: : This document is Similarity Check deposited; : Supplementary Information; : Single-blind; : Received 13 September 2019; Accepted 9 October 2019; Accepted Manuscript published 10 October 2019
• Contract Date: Oct 11, 2019