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Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya

Ngamsom, Bongkot; Wandera, Ernest Apondi; Wandera, Ernest Apondi; Iles, Alexander; Kimani, Racheal; Muregi, Francis; Gitaka, Jesse; Pamme, Nicole

Authors

Bongkot Ngamsom

Ernest Apondi Wandera

Ernest Apondi Wandera

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Dr Alex Iles Alexander.Iles@hull.ac.uk
Experimental Officer - Lab on a Chip

Racheal Kimani

Francis Muregi

Jesse Gitaka



Abstract

© The Royal Society of Chemistry 2019. We report the rapid detection (20 min) of Streptococcus agalactiae, Group B Streptococcus (GBS) employing on-chip magnetic isolation of GBS based on immiscible filtration assisted by surface tension (IFAST), followed by detection of the isolated GBS using an adenosine triphosphate (ATP) bioluminescence assay. Up to 80% GBS cells were isolated from spiked artificial urine samples with linear responses of bioluminescence signals from isolated cells at 2.3 × 102-9.1 × 105 CFU mL-1, demonstrating great promise for point-of-care detection of pathogenic bacteria in screening urine samples from pregnant women. Practical challenges during initial testing of the developed protocol with urine samples in Kenya are also described.

Citation

Ngamsom, B., Wandera, E. A., Iles, A., Kimani, R., Muregi, F., Gitaka, J., & Pamme, N. (2019). Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya. Analyst, 144(23), 6889-6897 . https://doi.org/10.1039/c9an01808e

Journal Article Type Article
Acceptance Date Oct 9, 2019
Online Publication Date Oct 10, 2019
Publication Date Oct 10, 2019
Deposit Date Oct 11, 2019
Publicly Available Date Oct 11, 2020
Journal Analyst
Print ISSN 0003-2654
Electronic ISSN 1364-5528
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 144
Issue 23
Pages 6889-6897
DOI https://doi.org/10.1039/c9an01808e
Keywords Analytical Chemistry; Spectroscopy; Electrochemistry; Biochemistry; Environmental Chemistry
Public URL https://hull-repository.worktribe.com/output/2909690
Publisher URL https://pubs.rsc.org/en/Content/ArticleLanding/2019/AN/C9AN01808E#!divAbstract
Additional Information : This document is Similarity Check deposited; : Supplementary Information; : Single-blind; : Received 13 September 2019; Accepted 9 October 2019; Accepted Manuscript published 10 October 2019

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