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A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis

Ngamsom, Bongkot; Truyts, Alma; Fourie, Louis; Kumar, Shavon; Tarn, Mark D.; Iles, Alexander; Moodley, Klariska; Land, Kevin J.; Pamme, Nicole

Authors

Bongkot Ngamsom

Alma Truyts

Louis Fourie

Shavon Kumar

Mark D. Tarn

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Dr Alex Iles a.Iles@hull.ac.uk
Experimental Officer - Lab on a Chip

Klariska Moodley

Kevin J. Land



Abstract

© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim We present a simple microfluidic system for rapid screening of Escherichia coli (E. coli) O157:H7 employing the specificity of immunomagnetic separation (IMS) via immiscible filtration assisted by sur face tension (IFAST), and the sensitivity of the subsequent adenosine triphosphate (ATP) assay by the bioluminescence luciferin/luciferase reaction. The developed device was capable of detecting E. coli O157:H7 from just 6 colony forming units (CFU) in 1 mL spiked buffer within 20 min. When tested with wastewater discharged effluent samples, without pre-concentration, the device demonstrated the ability to detect 10 4 CFU per mL seeded; suggesting great potential for point-of-need microbiological water quality monitoring.

Citation

Ngamsom, B., Truyts, A., Fourie, L., Kumar, S., Tarn, M. D., Iles, A., …Pamme, N. (2017). A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis. Chemistry : a European journal, 23(52), 12754-12757. https://doi.org/10.1002/chem.201703487

Journal Article Type Article
Acceptance Date Jul 27, 2017
Online Publication Date Aug 18, 2017
Publication Date Sep 18, 2017
Deposit Date Aug 2, 2017
Publicly Available Date Jul 31, 2018
Journal Chemistry - a European journal
Print ISSN 0947-6539
Electronic ISSN 1521-3765
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 23
Issue 52
Pages 12754-12757
DOI https://doi.org/10.1002/chem.201703487
Keywords ATP assays; E. coli; Immunoassays; Luminescence; Water chemistry
Public URL https://hull-repository.worktribe.com/output/453863
Publisher URL http://onlinelibrary.wiley.com/doi/10.1002/chem.201703487/abstract
Copyright Statement ©2018 University of Hull
Additional Information This is the accepted manuscript of an article published in Chemistry - an European journal, 2017. The version of record is available at the DOI link in this record.

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