A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis

Ngamsom, Bongkot; Truyts, Alma; Fourie, Louis; Kumar, Shavon; Tarn, Mark D.; Iles, Alexander; Moodley, Klariska; Land, Kevin J.; Pamme, Nicole

Abstract

© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim We present a simple microfluidic system for rapid screening of Escherichia coli (E. coli) O157:H7 employing the specificity of immunomagnetic separation (IMS) via immiscible filtration assisted by sur face tension (IFAST), and the sensitivity of the subsequent adenosine triphosphate (ATP) assay by the bioluminescence luciferin/luciferase reaction. The developed device was capable of detecting E. coli O157:H7 from just 6 colony forming units (CFU) in 1 mL spiked buffer within 20 min. When tested with wastewater discharged effluent samples, without pre-concentration, the device demonstrated the ability to detect 10 4 CFU per mL seeded; suggesting great potential for point-of-need microbiological water quality monitoring.

Citation

Ngamsom, B., Truyts, A., Fourie, L., Kumar, S., Tarn, M. D., Iles, A., …Pamme, N. (2017). A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis. Chemistry: a European journal, 23(52), 12754-12757. https://doi.org/10.1002/chem.201703487

• Journal Article Type: Article
• Acceptance Date: Jul 27, 2017
• Online Publication Date: Aug 18, 2017
• Publication Date: Sep 18, 2017
• Deposit Date: Aug 2, 2017
• Publicly Available Date: Jul 31, 2018
• Journal: Chemistry - a European journal
• Print ISSN: 0947-6539
• Electronic ISSN: 1521-3765
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 23
• Issue: 52
• Pages: 12754-12757
• DOI: https://doi.org/10.1002/chem.201703487
• Keywords: ATP assays; E. coli; Immunoassays; Luminescence; Water chemistry
• Public URL: https://hull-repository.worktribe.com/output/453863
• Publisher URL: http://onlinelibrary.wiley.com/doi/10.1002/chem.201703487/abstract
• Additional Information: This is the accepted manuscript of an article published in Chemistry - an European journal, 2017. The version of record is available at the DOI link in this record.