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A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis

Ngamsom, Bongkot; Truyts, Alma; Fourie, Louis; Kumar, Shavon; Tarn, Mark D.; Iles, Alexander; Moodley, Klariska; Land, Kevin J.; Pamme, Nicole

Authors

Bongkot Ngamsom

Alma Truyts

Louis Fourie

Shavon Kumar

Mark D. Tarn

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Dr Alex Iles Alexander.Iles@hull.ac.uk
Experimental Officer - Lab on a Chip

Klariska Moodley

Kevin J. Land



Abstract

© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim We present a simple microfluidic system for rapid screening of Escherichia coli (E. coli) O157:H7 employing the specificity of immunomagnetic separation (IMS) via immiscible filtration assisted by sur face tension (IFAST), and the sensitivity of the subsequent adenosine triphosphate (ATP) assay by the bioluminescence luciferin/luciferase reaction. The developed device was capable of detecting E. coli O157:H7 from just 6 colony forming units (CFU) in 1 mL spiked buffer within 20 min. When tested with wastewater discharged effluent samples, without pre-concentration, the device demonstrated the ability to detect 10 4 CFU per mL seeded; suggesting great potential for point-of-need microbiological water quality monitoring.

Journal Article Type Article
Publication Date Sep 18, 2017
Journal Chemistry - a European journal
Print ISSN 0947-6539
Electronic ISSN 1521-3765
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 23
Issue 52
Pages 12754-12757
Institution Citation Ngamsom, B., Truyts, A., Fourie, L., Kumar, S., Tarn, M. D., Iles, A., …Pamme, N. (2017). A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis. Chemistry : a European journal, 23(52), 12754-12757. https://doi.org/10.1002/chem.201703487
DOI https://doi.org/10.1002/chem.201703487
Keywords ATP assays; E. coli; Immunoassays; Luminescence; Water chemistry
Publisher URL http://onlinelibrary.wiley.com/doi/10.1002/chem.201703487/abstract
Copyright Statement ©2018 University of Hull
Additional Information This is the accepted manuscript of an article published in Chemistry - an European journal, 2017. The version of record is available at the DOI link in this record.

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