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Effect of Particle Wettability and Particle Concentration on the Enzymatic Dehydration of n-Octanaloxime in Pickering Emulsions

Bago Rodriguez, Ana Maria; Schober, Lukas; Hinzmann, Alessa; Gröger, Harald; Binks, Bernard P.

Authors

Ana Maria Bago Rodriguez

Lukas Schober

Alessa Hinzmann

Harald Gröger



Abstract

© 2020 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH Pickering emulsion systems have emerged as platforms for the synthesis of organic molecules in biphasic biocatalysis. Herein, the catalytic performance was evaluated for biotransformation using whole cells exemplified for the dehydration of n-octanaloxime to n-octanenitrile catalysed by an aldoxime dehydratase (OxdB) overexpressed in E. coli. This study was carried out in Pickering emulsions stabilised solely with silica particles of different hydrophobicity. We correlate, for the first time, the properties of the emulsions with the conversion of the reaction, thus gaining an insight into the impact of the particle wettability and particle concentration. When comparing two emulsions of different type with similar stability and droplet diameter, the oil-in-water (o/w) system displayed a higher conversion than the water-in-oil (w/o) system, despite the conversion in both cases being higher than that in a “classic” two-phase system. Furthermore, an increase in particle concentration prior to emulsification resulted in an increase of the interfacial area and hence a higher conversion.

Citation

Bago Rodriguez, A. M., Schober, L., Hinzmann, A., Gröger, H., & Binks, B. P. (2020). Effect of Particle Wettability and Particle Concentration on the Enzymatic Dehydration of n-Octanaloxime in Pickering Emulsions. Angewandte Chemie, 59, https://doi.org/10.1002/anie.202013171

Journal Article Type Article
Acceptance Date Oct 28, 2020
Online Publication Date Dec 21, 2020
Publication Date 2020
Deposit Date Dec 2, 2020
Publicly Available Date Dec 21, 2020
Journal Angewandte Chemie - International Edition
Print ISSN 1433-7851
Electronic ISSN 1521-3773
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 59
DOI https://doi.org/10.1002/anie.202013171
Keywords Pickering emulsions; Silica particles; Biphasic catalysis; Enzyme catalysis
Public URL https://hull-repository.worktribe.com/output/3669941
Publisher URL https://onlinelibrary.wiley.com/doi/10.1002/anie.202013171
Additional Information Received: 2020-09-29; Accepted: 2020-10-28; Published: 2020-10-29

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