Effect of Particle Wettability and Particle Concentration on the Enzymatic Dehydration of n-Octanaloxime in Pickering Emulsions

Bago Rodriguez, Ana Maria; Schober, Lukas; Hinzmann, Alessa; Gröger, Harald; Binks, Bernard P.

Abstract

© 2020 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH Pickering emulsion systems have emerged as platforms for the synthesis of organic molecules in biphasic biocatalysis. Herein, the catalytic performance was evaluated for biotransformation using whole cells exemplified for the dehydration of n-octanaloxime to n-octanenitrile catalysed by an aldoxime dehydratase (OxdB) overexpressed in E. coli. This study was carried out in Pickering emulsions stabilised solely with silica particles of different hydrophobicity. We correlate, for the first time, the properties of the emulsions with the conversion of the reaction, thus gaining an insight into the impact of the particle wettability and particle concentration. When comparing two emulsions of different type with similar stability and droplet diameter, the oil-in-water (o/w) system displayed a higher conversion than the water-in-oil (w/o) system, despite the conversion in both cases being higher than that in a “classic” two-phase system. Furthermore, an increase in particle concentration prior to emulsification resulted in an increase of the interfacial area and hence a higher conversion.

Citation

Bago Rodriguez, A. M., Schober, L., Hinzmann, A., Gröger, H., & Binks, B. P. (2020). Effect of Particle Wettability and Particle Concentration on the Enzymatic Dehydration of n-Octanaloxime in Pickering Emulsions. Angewandte Chemie, 59, https://doi.org/10.1002/anie.202013171

• Journal Article Type: Article
• Acceptance Date: Oct 28, 2020
• Online Publication Date: Dec 21, 2020
• Publication Date: 2020
• Deposit Date: Dec 2, 2020
• Publicly Available Date: Dec 21, 2020
• Journal: Angewandte Chemie - International Edition
• Print ISSN: 1433-7851
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 59
• DOI: https://doi.org/10.1002/anie.202013171
• Keywords: Pickering emulsions; Silica particles; Biphasic catalysis; Enzyme catalysis
• Public URL: https://hull-repository.worktribe.com/output/3669941
• Publisher URL: https://onlinelibrary.wiley.com/doi/10.1002/anie.202013171