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The on-line synthesis of enzyme functionalized silica nanoparticles in a microfluidic reactor using polyethylenimine polymer and R5 peptide

He, Ping; Greenway, Gillian; Haswell, Stephen J

Authors

Ping He P.He@hull.ac.uk

Gillian Greenway G.M.Greenway@hull.ac.uk

Stephen J Haswell



Abstract

A simple microfluidic reactor system is described for the effective synthesis of enzyme functionalized nanoparticles which offers many advantages over batch reactions, including excellent enzyme efficiencies. Better control of the process parameters in the microfluidic reactor system over batch based methodology enables the production of silica nanoparticles with the optimum size for efficient enzyme immobilization with long-term stability. The synthetic approach is demonstrated with glucose oxidase (GOD) and two different nucleation catalysts of similar molecular mass: the natural R5 peptide, and polyethylenimine (PEI) polymer. Near-quantitative immobilization of GOD in the nanoparticles is obtained using PEI; the immobilization is attributed to electrostatic interaction between PEI and GOD. This interaction, however, limits the mobility of the immobilized enzyme, producing orientation hindrance of the enzyme's active sites as compared to free GOD in solution. In contrast, when the GOD is immobilized inside the silica nanoparticles using R5, lower enzyme immobilization efficiencies are obtained compared to using PEI polymers; however, similar Michaelis -Menten kinetic parameters (i.e. Michaelis constant and turnover number) to those of free GOD are observed. Reactions were monitored in situ using simple, rapid, separation-free amperometric detection.

Journal Article Type Article
Publication Date Aug 6, 2008
Journal NANOTECHNOLOGY
Print ISSN 0957-4484
Electronic ISSN 1361-6528
Publisher IOP Publishing
Peer Reviewed Peer Reviewed
Volume 19
Issue 31
Article Number 315603
Pages 315603
APA6 Citation He, P., Greenway, G., & Haswell, S. J. (2008). The on-line synthesis of enzyme functionalized silica nanoparticles in a microfluidic reactor using polyethylenimine polymer and R5 peptide. Nanotechnology, 19(31), 315603. https://doi.org/10.1088/0957-4484/19/31/315603
DOI https://doi.org/10.1088/0957-4484/19/31/315603
Keywords Mechanical Engineering; Electrical and Electronic Engineering; General Materials Science; Mechanics of Materials; Bioengineering; General Chemistry