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Microparticle-associated vascular adhesion molecule-1 and tissue factor follow a circadian rhythm in healthy human subjects

Madden, Leigh A.; Vince, Rebecca V.; Sandström, Marie E.; Taylor, Lee; McNaughton, Lars; Laden, Gerard


Marie E. Sandström

Lee Taylor

Lars McNaughton

Gerard Laden


An increased risk of death or severe injury due to late-morning thrombotic events is well established. Tissue factor (TF) is the initiator of the coagulation cascade, and endothelial stresses, coupled with production of pro-coagulant microparticles (MP) are also important factors in loss of haemostasis. TF and vascular cell adhesion molecule-1 (VCAM-1) -positive cell microparticles were assessed periodically over a 24-hour (h) period in healthy human subjects to ascertain if they followed a circadian rhythm. Eleven healthy male subjects were assessed in a temperature- controlled environment with dietary intake consistent between subjects. Blood samples were taken every 4 h by venipuncture, and TF and VCAM-1 positive microparticles were quantified by flow cytometry. A significant circadian rhythm was observed in VCAM-1 MP (p=<0.0001), and a trend was shown, although not statistically significant (p=0.065) in TF microparticles. A peak was observed at 9 a.m. for VCAM-1 positive MP, followed by a decrease and subsequent peak at 9 p.m. and a minimum at 5 a.m. TF-positive MP followed a strikingly similar trend in both variation and absolute numbers with a delay. A circadian rhythm was observed in VCAM-1 and less so TF-positive MP. This has significant implications in terms of the well known increased risk of cardiovascular thrombotic events matching this data. To our knowledge this is the first such report of quantified measurements of these MP over a 24-h period and the only measurement of a 24-h variation of in-vivo blood-borne TF.


Madden, L. A., Vince, R. V., Sandström, M. E., Taylor, L., McNaughton, L., & Laden, G. (2008). Microparticle-associated vascular adhesion molecule-1 and tissue factor follow a circadian rhythm in healthy human subjects. Thrombosis and haemostasis, 99(5), 909 - 915.

Journal Article Type Article
Acceptance Date Mar 23, 2008
Online Publication Date Nov 30, 2017
Publication Date Apr 11, 2008
Journal Thrombosis and Haemostasis
Print ISSN 0340-6245
Publisher Schattauer
Peer Reviewed Peer Reviewed
Volume 99
Issue 5
Pages 909 - 915
Keywords Circadian rhythm; Microparticles; Tissue factor; Vascular cell adhesion molecule-1
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