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Ocean acidification affects marine chemical communication by changing structure and function of peptide signalling molecules

Roggatz, Christina C.; Lorch, Mark; Hardege, Jörg D.; Benoit, David M.

Authors

Christina C. Roggatz

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Dr David Benoit D.Benoit@hull.ac.uk
Senior Lecturer in Molecular Physics and Astrochemistry

Abstract

Ocean acidification is a global challenge that faces marine organisms in the near future with a predicted rapid drop in pH of up to 0.4 units by the end of this century. Effects of the change in ocean carbon chemistry and pH on the development, growth and fitness of marine animals are well documented. Recent evidence also suggests that a range of chemically mediated behaviours and interactions in marine fish and invertebrates will be affected. Marine animals use chemical cues, for example, to detect predators, for settlement, homing and reproduction. But while effects of high CO₂ conditions on these behaviours are described across many species, little is known about the underlying mechanisms, particularly in invertebrates. Here we investigate the direct influence of future oceanic pH conditions on the structure and function of three peptide signalling molecules with an interdisciplinary combination of methods. NMR spectroscopy and quantum chemical calculations were used to assess the direct molecular influence of pH on the peptide cues and we tested the functionality of the cues in different pH conditions using behavioural bioassays with shore crabs (Carcinus maenas) as a model system. We found that peptide signalling cues are susceptible to protonation in future pH conditions, which will alter their overall charge. We also show that structure and electrostatic properties important for receptor-binding differ significantly between the peptide forms present today and the protonated signalling peptides likely to be dominating in future oceans. The bioassays suggest an impaired functionality of the signalling peptides at low pH. Physiological changes due to high CO₂ conditions were found to play a less significant role in influencing the investigated behaviour. From our results we conclude that the change of charge, structure and consequently function of signalling molecules presents one possible mechanism to explain altered behaviour under future oceanic pH conditions.

Publication Date 2016-12
Journal Global change biology
Print ISSN 1354-1013
Electronic ISSN 1365-2486
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 22
Issue 12
Pages 3914-3926
Institution Citation Roggatz, C. C., Lorch, M., Hardege, J. D., & Benoit, D. M. (2016). Ocean acidification affects marine chemical communication by changing structure and function of peptide signalling molecules. Global change biology, 22(12), 3914-3926. https://doi.org/10.1111/gcb.13354
DOI https://doi.org/10.1111/gcb.13354
Keywords CO₂; Carbon dioxide; GGR; Gly-Gly-Arg; Glycyl-43 glycyl-L-arginine; GHK; Gly-His-Lys; Glycyl-L-histidyl-L-lysine; LR; Leu-Arg; L-leucyl-L-arginine; NMR; Nuclear magnetic resonance
Publisher URL http://onlinelibrary.wiley.com/doi/10.1111/gcb.13354/abstract
Copyright Statement ©2017 University of Hull

Authors' accepted manuscript of article: Roggatz, C. C., Lorch, M., Hardege, J. D. and Benoit, D. M. (2016), Ocean acidification affects marine chemical communication by changing structure and function of peptide signalling molecules. Glob Change Biol, 22: 3914–3926. doi:10.1111/gcb.13354
Additional Information Authors' accepted manuscript of article: Roggatz, C. C., Lorch, M., Hardege, J. D. and Benoit, D. M. (2016), Ocean acidification affects marine chemical communication by changing structure and function of peptide signalling molecules. Glob Change Biol, 22: 3914–3926. doi:10.1111/gcb.13354

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Copyright Statement
©2017 University of Hull

Authors' accepted manuscript of article: Roggatz, C. C., Lorch, M., Hardege, J. D. and Benoit, D. M. (2016), Ocean acidification affects marine chemical communication by changing structure and function of peptide signalling molecules. Glob Change Biol, 22: 3914–3926. doi:10.1111/gcb.13354





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