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Increased inducible heat shock protein 72 expression associated with PBMC isolated from patients with haematological tumours

Madden, Leigh A.; Hayman, Yvette A.; Underwood, Corrinna; Vince, Rebecca V.; Greenman, John; Allsup, David; Ali, Sahra

Authors

Yvette A. Hayman

Corrinna Underwood

Sahra Ali



Abstract

Background. Heat shock protein 72 (Hsp72) is a highly inducible stress protein and molecular chaperone. Cancers have been shown to be associated with increased Hsp72 expression within the tumour itself and this may lead to resistance to apoptosis. Methods. Peripheral blood mononuclear cells (PBMC) were isolated from patients diagnosed with chronic lymphocytic leukaemia (CLL) (n 27) and chronic myelomonocytic leukaemia (CMML) (n 16) and Hsp72 expression was characterized on both the cell surface and intracellularly by flow cytometry. To allow for comparison PBMC from breast cancer patients (n 25) and healthy volunteers (n 19) were included. Results. Both lymphocytes and monocytes from CLL and CMML patients showed high levels of total Hsp72 expression (46 fold increase) in comparison to breast cancer and healthy subjects. The majority of Hsp72 in these tumours was determined to be cell-surface expressed (6493% of cell total Hsp72). Conclusions. A correlation was observed between lymphocyte and monocyte total Hsp72 expression (p < 0.001) suggesting a common stress response pathway may exist in these blood cells and there are stress conditions present within the circulation. Hsp72 expression was not found to be related to white blood cell count. © 2012 Informa Healthcare.

Citation

Madden, L. A., Hayman, Y. A., Underwood, C., Vince, R. V., Greenman, J., Allsup, D., & Ali, S. (2012). Increased inducible heat shock protein 72 expression associated with PBMC isolated from patients with haematological tumours. Scandinavian journal of clinical & laboratory investigation, 72(5), 380-386. https://doi.org/10.3109/00365513.2012.681683

Journal Article Type Article
Acceptance Date Mar 27, 2012
Online Publication Date May 1, 2012
Publication Date Sep 1, 2012
Deposit Date Apr 4, 2022
Journal Scandinavian Journal of Clinical and Laboratory Investigation
Print ISSN 0036-5513
Publisher Taylor and Francis
Peer Reviewed Peer Reviewed
Volume 72
Issue 5
Pages 380-386
DOI https://doi.org/10.3109/00365513.2012.681683
Keywords Heat shock protein; hsp72; Leukaemia; Monocyte; Lymphocyte
Public URL https://hull-repository.worktribe.com/output/520922