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Paper-based analytical devices for colorimetric detection of: S. aureus and E. coli and their antibiotic resistant strains in milk (2020)
Journal Article
Khan, Q. M., Asif, M., Awan, F. R., Khan, Q. . M., Ngamsom, B., & Pamme, N. (2020). Paper-based analytical devices for colorimetric detection of: S. aureus and E. coli and their antibiotic resistant strains in milk. Analyst, 145(22), 7320-7329. https://doi.org/10.1039/d0an01075h

Animal derived milk which is an important part of human diet due to its high nutritional value not only supports humans but also presents a growth environment for pathogenic bacteria. Milk may become contaminated with bacteria through udder infection... Read More about Paper-based analytical devices for colorimetric detection of: S. aureus and E. coli and their antibiotic resistant strains in milk.

Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya (2019)
Journal Article
Ngamsom, B., Wandera, E. A., Iles, A., Kimani, R., Muregi, F., Gitaka, J., & Pamme, N. (2019). Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya. Analyst, 144(23), 6889-6897. https://doi.org/10.1039/c9an01808e

© The Royal Society of Chemistry 2019. We report the rapid detection (20 min) of Streptococcus agalactiae, Group B Streptococcus (GBS) employing on-chip magnetic isolation of GBS based on immiscible filtration assisted by surface tension (IFAST), fol... Read More about Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya.

A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis (2017)
Journal Article
Ngamsom, B., Truyts, A., Fourie, L., Kumar, S., Tarn, M. D., Iles, A., Moodley, K., Land, K. J., & Pamme, N. (2017). A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis. Chemistry: a European journal, 23(52), 12754-12757. https://doi.org/10.1002/chem.201703487

© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim We present a simple microfluidic system for rapid screening of Escherichia coli (E. coli) O157:H7 employing the specificity of immunomagnetic separation (IMS) via immiscible filtration assisted by s... Read More about A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis.

Multiplex sorting of foodborne pathogens by on-chip free-flow magnetophoresis (2016)
Journal Article
Ngamsom, B., Esfahani, M. M., Phurimsak, C., Lopez-Martinez, M. J., Raymond, J.-C., Broyer, P., Patel, P., & Pamme, N. (2016). Multiplex sorting of foodborne pathogens by on-chip free-flow magnetophoresis. Analytica Chimica Acta, 918, 69-76. https://doi.org/10.1016/j.aca.2016.03.014

This study reports multiplex sorting of Salmonella typhimurium and Escherichia coli 0157, from broth cultures and from pathogen-spiked skinned chicken breast enrichment broths by employing microfluidic free-flow magnetophoresis. Magnetic beads of dif... Read More about Multiplex sorting of foodborne pathogens by on-chip free-flow magnetophoresis.

On-chip acoustophoretic isolation of microflora including S. typhimurium from raw chicken, beef and blood samples (2016)
Journal Article
Ngamsom, B., Lopez-Martinez, M. J., Raymond, J.-C., Broyer, P., Patel, P., & Pamme, N. (2016). On-chip acoustophoretic isolation of microflora including S. typhimurium from raw chicken, beef and blood samples. Journal of microbiological methods, 123, 79-86. https://doi.org/10.1016/j.mimet.2016.01.016

Pathogen analysis in food samples routinely involves lengthy growth-based pre-enrichment and selective enrichment of food matrices to increase the ratio of pathogen to background flora. Similarly, for blood culture analysis, pathogens must be isolate... Read More about On-chip acoustophoretic isolation of microflora including S. typhimurium from raw chicken, beef and blood samples.

The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flow (2010)
Journal Article
Ngamsom, B., Hickey, A. M., Greenway, G. M., Littlechild, J. A., McCreedy, T., Watts, P., & Wiles, C. (2010). The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flow. Organic & biomolecular chemistry, 8(10), 2419-2424. https://doi.org/10.1039/b924100k

Through the preparation of a novel controlled pore glass-poly(pyrrole) material we have developed a conducting support that is not only suitable for the co-immobilisation of enzymes and co-factors, but also enables the facile electrochemical regenera... Read More about The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flow.

Development of a high throughput screening tool for biotransformations utilising a thermophilic L-aminoacylase enzyme (2009)
Journal Article
Ngamsom, B., Hickey, A. M., Greenway, G. M., Littlechild, J. A., Watts, P., & Wiles, C. (2010). Development of a high throughput screening tool for biotransformations utilising a thermophilic L-aminoacylase enzyme. Journal of Molecular Catalysis B: Enzymatic, 63(1-2), 81-86. https://doi.org/10.1016/j.molcatb.2009.12.013

Micro-reactors containing a monolith-immobilised thermophilic l-aminoacylase, from Thermococcus litoralis, have been developed for use in biotransformation reactions and a study has been carried out to investigate the stereospecificity and stability... Read More about Development of a high throughput screening tool for biotransformations utilising a thermophilic L-aminoacylase enzyme.