All Outputs (85)

Microfluidic-Based Electrochemical Immunosensing of Ferritin (2020)
Journal Article
Garg, M., Christensen, M. G., Iles, A., Sharma, A. L., Singh, S., & Pamme, N. (2020). Microfluidic-Based Electrochemical Immunosensing of Ferritin. Biosensors, 10(8), Article 91. https://doi.org/10.3390/bios10080091

Ferritin is a clinically important biomarker which reflects the state of iron in the body and is directly involved with anemia. Current methods available for ferritin estimation are generally not portable or they do not provide a fast response. To co... Read More about Microfluidic-Based Electrochemical Immunosensing of Ferritin.

On-chip electrochemical detection of glucose towards the miniaturised quality control of carbohydrate-based radiotracers (2020)
Journal Article
Patinglag, L., Esfahani, M. M. N., Ragunathan, K., He, P., Brown, N. J., Archibald, S. J., Pamme, N., & Tarn, M. D. (2020). On-chip electrochemical detection of glucose towards the miniaturised quality control of carbohydrate-based radiotracers. Analyst, 145(14), 4920-4930. https://doi.org/10.1039/c9an01881f

The miniaturisation of positron emission tomography (PET) radiotracer production is facilitating a move towards a dose-on-demand strategy that would enable a stratified approach to patient diagnostics, but while the on-chip synthesis steps have been... Read More about On-chip electrochemical detection of glucose towards the miniaturised quality control of carbohydrate-based radiotracers.

Realisation of a sub-wavelength dimple using a 193 nm wavelength photonic nano jet (2020)
Journal Article
Al-Jarwany, Q. A., Mohammed, A. F., Hamza, A. O., Bouillard, J. S., Adawi, A. M., Pamme, N., & Walton, C. D. (2020). Realisation of a sub-wavelength dimple using a 193 nm wavelength photonic nano jet. Chemical Physics Letters, 750, Article 137400. https://doi.org/10.1016/j.cplett.2020.137400

There are many areas of research that benefit from tight focussing of light. We report experimental and computational results of a laser irradiated silica microsphere, 1 μm diameter localised on an SU-8 substrate. A single pulse from an ArF excimer l... Read More about Realisation of a sub-wavelength dimple using a 193 nm wavelength photonic nano jet.

Biosensor for determining average iron content of ferritin by measuring its optical dispersion (2020)
Presentation / Conference Contribution
Gupta, R., Alamrani, N. A., Greenway, G. M., Pamme, N., & Goddard, N. J. Biosensor for determining average iron content of ferritin by measuring its optical dispersion. Presented at SPIE BIOS: Microfluidics, BioMEMS, and Medical Microsystems XVIII, San Francisco, California

© 2020 SPIE. Average iron content of ferritin has a potential to serve as a biomarker for early identification of high-risk trauma patients at point-of-care (PoC). Appropriate therapies can then be administered to reduce morbidity and mortality. Curr... Read More about Biosensor for determining average iron content of ferritin by measuring its optical dispersion.

The use of precision cut lung slices and co-culture modelling to investigate the effect of human rhinovirus on cough and airway inflammation (2020)
Thesis
Stinson, R. J. The use of precision cut lung slices and co-culture modelling to investigate the effect of human rhinovirus on cough and airway inflammation. (Thesis). Hull York Medical School, the University of Hull and the University of York. https://hull-repository.worktribe.com/output/4222863

Human Rhinovirus (hRV) is a major cause of upper respiratory tract infections (URTIs) and is linked to lower respiratory tract infections (LRTIs) and airway diseases exacerbations. Cough, airway inflammation and hypersensation are common symptoms of... Read More about The use of precision cut lung slices and co-culture modelling to investigate the effect of human rhinovirus on cough and airway inflammation.

FISH and chips: a review of microfluidic platforms for FISH analysis (2020)
Journal Article
Rodriguez-Mateos, P., Azevedo, N. F., Almeida, C., & Pamme, N. (2020). FISH and chips: a review of microfluidic platforms for FISH analysis. Medical Microbiology and Immunology, 209(3), 373-391. https://doi.org/10.1007/s00430-019-00654-1

Fluorescence in situ hybridization (FISH) allows visualization of specific nucleic acid sequences within an intact cell or a tissue section. It is based on molecular recognition between a fluorescently labeled probe that penetrates the cell membrane... Read More about FISH and chips: a review of microfluidic platforms for FISH analysis.

Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya (2019)
Journal Article
Ngamsom, B., Wandera, E. A., Iles, A., Kimani, R., Muregi, F., Gitaka, J., & Pamme, N. (2019). Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya. Analyst, 144(23), 6889-6897. https://doi.org/10.1039/c9an01808e

© The Royal Society of Chemistry 2019. We report the rapid detection (20 min) of Streptococcus agalactiae, Group B Streptococcus (GBS) employing on-chip magnetic isolation of GBS based on immiscible filtration assisted by surface tension (IFAST), fol... Read More about Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya.

A feasibility study of a leaky waveguide aptasensor for thrombin (2019)
Journal Article
Alamrani, N. A., Greenway, G. M., Pamme, N., Goddard, N. J., & Gupta, R. (2019). A feasibility study of a leaky waveguide aptasensor for thrombin. Analyst, 144(20), 6048-6054. https://doi.org/10.1039/c9an01421g

This proof-of-principle study demonstrates the feasibility of a leaky waveguide (LW) aptasensor, where aptamers were immobilised in a mesoporous chitosan waveguiding film for the detection of thrombin. This work has demonstrated that aptamers immobil... Read More about A feasibility study of a leaky waveguide aptasensor for thrombin.

“Learning on a chip:” Microfluidics for formal and informal science education (2019)
Journal Article
Rackus, D. G., Riedel-Kruse, I. H., & Pamme, N. (2019). “Learning on a chip:” Microfluidics for formal and informal science education. Biomicrofluidics, 13(4), Article 041501. https://doi.org/10.1063/1.5096030

© 2019 Author(s). Microfluidics is a technique for the handling of small volumes of liquids on the order of picoliters to nanoliters and has impact for miniaturized biomedical science and fundamental research. Because of its multi- and interdisciplin... Read More about “Learning on a chip:” Microfluidics for formal and informal science education.

An optical label-free aptasensor based on dye doped leaky waveguide (DDLW) for biomarker detection (2019)
Thesis
Alamrani, N. A. An optical label-free aptasensor based on dye doped leaky waveguide (DDLW) for biomarker detection. (Thesis). University of Hull. https://hull-repository.worktribe.com/output/4749483

Due to the rapid increase in routine measurement of biomarkers, such as a protein, in blood samples for healthcare monitoring, different techniques have been developed to facilitate this measurement in terms of cost-effective and fast measurement. La... Read More about An optical label-free aptasensor based on dye doped leaky waveguide (DDLW) for biomarker detection.

A method for determining average iron content of ferritin by measuring its optical dispersion (2019)
Journal Article
Gupta, R., Alamrani, N. A., Greenway, G. M., Pamme, N., & Goddard, N. J. (2019). A method for determining average iron content of ferritin by measuring its optical dispersion. Analytical chemistry, 91(11), 7366-7372. https://doi.org/10.1021/acs.analchem.9b01231

© 2019 American Chemical Society. We report a method where the refractive index increments of an iron storage protein, ferritin, and apoferritin (ferritin minus iron) were measured over the wavelength range of 450-678 nm to determine the average iron... Read More about A method for determining average iron content of ferritin by measuring its optical dispersion.

Developing a novel spheroid-on-chip microfluidic device for investigations into metastasis (2019)
Thesis
Collins, T. C. Developing a novel spheroid-on-chip microfluidic device for investigations into metastasis. (Thesis). University of Hull. https://hull-repository.worktribe.com/output/4221851

One in two people born after 1960 will develop cancer, with 90% of all cancer deaths arising from metastasis. Conventional 2D in vitro metastasis models do not fully replicate tumour complexity. In vivo models can address tumour complexity, but do no... Read More about Developing a novel spheroid-on-chip microfluidic device for investigations into metastasis.

Two-step numerical approach to predict ferrofluid droplet generation and manipulation inside multilaminar flow chambers (2019)
Journal Article
Gómez-Pastora, J., Amiri Roodan, V., Karampelas, I. H., Alorabi, A. Q., Tarn, M. D., Iles, A., Bringas, E., Paunov, V. N., Pamme, N., Furlani, E. P., & Ortiz, I. (2019). Two-step numerical approach to predict ferrofluid droplet generation and manipulation inside multilaminar flow chambers. Journal of physical chemistry. C, 123(15), 10065-10080. https://doi.org/10.1021/acs.jpcc.9b01393

Copyright © 2019 American Chemical Society. In this study, a computational fluid dynamics approach is implemented to investigate the dynamic behavior of continuous-flow droplet microfluidics. The developed approach predicts both droplet generation an... Read More about Two-step numerical approach to predict ferrofluid droplet generation and manipulation inside multilaminar flow chambers.

Stereolithographic 3D printing of extrinsically self-healing composites (2019)
Journal Article
Sanders, P., Young, A. J., Qin, Y., Fancey, K. S., Reithofer, M. R., Guillet-Nicolas, R., Kleitz, F., Pamme, N., & Chin, J. M. (2019). Stereolithographic 3D printing of extrinsically self-healing composites. Scientific reports, 9(1), Article 388. https://doi.org/10.1038/s41598-018-36828-9

We demonstrate for the first time the direct stereolithographic 3D printing of an extrinsically self-healing composite, comprised of commercial photocurable resin modified with anisole and PMMA-filled microcapsules. The composites demonstrate solvent... Read More about Stereolithographic 3D printing of extrinsically self-healing composites.

A label-free aptamer-based nanogap capacitive biosensor with greatly diminished electrode polarization effects (2019)
Journal Article
Ghobaei Namhil, Z., Kemp, C., Verrelli, E., Iles, A., Pamme, N., Adawi, A. M., & Kemp, N. (2019). A label-free aptamer-based nanogap capacitive biosensor with greatly diminished electrode polarization effects. Physical chemistry chemical physics : PCCP, 21(2), 681-691. https://doi.org/10.1039/c8cp05510f

A significant impediment to the use of impedance spectroscopy in bio-sensing is the electrode polarization effect that arises from the movement of free ions to the electrode-solution interface, forming an electrical double layer (EDL). The EDL screen... Read More about A label-free aptamer-based nanogap capacitive biosensor with greatly diminished electrode polarization effects.

Microcapsules as assay compartments formed through layer-by-layer deposition (2018)
Journal Article
Alorabi, A. Q., Tarn, M. D., Thomas, M., Paunov, V. N., & Pamme, N. (2018). Microcapsules as assay compartments formed through layer-by-layer deposition. Analytical Methods, 10(44), 5335-5340. https://doi.org/10.1039/c8ay02012d

We investigate the potential of using microcapsules, generated through layer-by-layer assembly, as reaction compartments for bioassays. A streptavidin-biotin and an esterase-fluorescein diacetate (FDA) assay were employed for proof-of-concept. Strept... Read More about Microcapsules as assay compartments formed through layer-by-layer deposition.

Plastic scintillator-based microfluidic devices for miniaturized detection of Positron Emission Tomography radiopharmaceuticals (2018)
Journal Article
Tarn, M. D., Kızılyer, N. Y., Esfahani, M. M. N., Joshi, P., Brown, N. J., Pamme, N., Jenkins, D. G., & Archibald, S. J. (2018). Plastic scintillator-based microfluidic devices for miniaturized detection of Positron Emission Tomography radiopharmaceuticals. Chemistry: a European journal, 24(52), 13749-13753. https://doi.org/10.1002/chem.201802395

A miniaturized radio-HPLC detector has been developed comprising a microfluidic device fabricated from plastic scintillator in combination with a silicon photomultiplier light sensor, and tested with samples containing a positron-emitting radionuclid... Read More about Plastic scintillator-based microfluidic devices for miniaturized detection of Positron Emission Tomography radiopharmaceuticals.

CFD analysis and experimental validation of magnetic droplet generation and deflection across multilaminar flow streams (2018)
Presentation / Conference Contribution
Gómez-Pastora, J., Karampelas, I. H., Alorabi, A. Q., Tarn, M. D., Bringas, E., Iles, A., Paunov, V. N., Pamme, N., Furlani, E. P., & Ortiz, I. (2018, June). CFD analysis and experimental validation of magnetic droplet generation and deflection across multilaminar flow streams. Presented at TechConnect Briefs 2018 - Advanced Materials

© 2018 OOSV. All rights reserved. The use of droplet-based microfluidic systems has increased in the last decade due to the advantages these systems present such as compatibility with many chemical and biological reagents, capability of performing a... Read More about CFD analysis and experimental validation of magnetic droplet generation and deflection across multilaminar flow streams.

Nanogap capacitive biosensor for label-free aptamer-based protein detection (2018)
Thesis
Namhil, Z. G. Nanogap capacitive biosensor for label-free aptamer-based protein detection. (Thesis). University of Hull. https://hull-repository.worktribe.com/output/4220514

Recent advances in nanotechnology offer a new platform for the label free detection of biomolecules at ultra-low concentrations. Nano biosensors are emerging as a powerful method of improving device performance whilst minimizing device size, cost and... Read More about Nanogap capacitive biosensor for label-free aptamer-based protein detection.

Optical sensor for the analysis of biomarkers (2018)
Thesis
Al-Shemary, R. S. Optical sensor for the analysis of biomarkers. (Thesis). University of Hull. https://hull-repository.worktribe.com/output/4220996

The continuous measurement of biomarkers such as proteins and hormones in blood and serum are the cornerstone of healthcare monitoring and optimising treatment plans. Often such biomarkers are present in minute concentrations. Conventional analysis m... Read More about Optical sensor for the analysis of biomarkers.