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Selective radiolabelling with 68Ga under mild conditions: a route towards a porphyrin PET/PDT theranostic agent (2018)
Journal Article
Yap, S. Y., Price, T. W., Savoie, H., Boyle, R. W., & Stasiuk, G. J. (2018). Selective radiolabelling with 68Ga under mild conditions: a route towards a porphyrin PET/PDT theranostic agent. Chemical communications : Chem comm / the Royal Society of Chemistry, 54(57), 7952-7954. doi:10.1039/c8cc03897j

A theranostic conjugate for use as a positron emission tomography (PET) radiotracer and as a photosensitiser for photodynamic therapy (PDT) has been synthesised. A water-soluble porphyrin was coupled with the bifunctional chelate, H4Dpaa.ga. This con... Read More

Photo-induced anticancer activity and singlet oxygen production of prodigiosenes (2018)
Journal Article
Savoie, H., Figliola, C., Marchal, E., Crabbe, B. W., Hallett-Tapley, G. L., Boyle, R. W., & Thompson, A. (2018). Photo-induced anticancer activity and singlet oxygen production of prodigiosenes. Photochemical & photobiological sciences : an international journal, 17(5), (599-606). doi:10.1039/c8pp00060c. ISSN 1474-905X

© 2018 The Royal Society of Chemistry and Owner Societies. The photo-induced cytotoxicity of prodigiosenes is reported. One prodigiosene represents a synthetic analogue of the natural product prodigiosin, and two are conjugated to molecules that targ... Read More

Synthesis and Characterization of Temperature-Sensitive and Chemically Cross-Linked Poly(N-isopropylacrylamide)/Photosensitizer Hydrogels for Applications in Photodynamic Therapy (2018)
Journal Article
Belali, S., Savoie, H., O’Brien, J. M., Cafolla, A. A., O’Connell, B., Karimi, A. R., …Senge, M. O. (2018). Synthesis and Characterization of Temperature-Sensitive and Chemically Cross-Linked Poly(N-isopropylacrylamide)/Photosensitizer Hydrogels for Applications in Photodynamic Therapy. Biomacromolecules, 19(5), (1592-1601). doi:10.1021/acs.biomac.8b00293. ISSN 1525-7797

Copyright © 2018 American Chemical Society. A novel poly(N-isopropylacrylamide) (PNIPAM) hydrogel containing different photosensitizers (protoporphyrin IX (PpIX), pheophorbide a (Pba), and protoporphyrin IX dimethyl ester (PpIX-DME)) has been synthes... Read More

pH-dependent modulation of reactivity in Ruthenium(II) organometallics (2018)
Journal Article
Prior, T. J., Savoie, H., Boyle, R. W., & Murray, B. S. (2018). pH-dependent modulation of reactivity in Ruthenium(II) organometallics. Organometallics, 37(3), (294-297). doi:10.1021/acs.organomet.7b00868. ISSN 0276-7333

The pH-dependent intramolecular chelation of a tethered sulfonamide ligand in ruthenium(II) arene complexes is demonstrated, a process shown to modulate metal-centered reactivity toward the model ligand guanosine 5′-monophosphate within the physiolog... Read More

Synthesis of a novel HER2 targeted aza-BODIPY–antibody conjugate: synthesis, photophysical characterisation and in vitro evaluation (2018)
Journal Article
Cheng, M. H. Y., Cheng, M. H., Maruani, A., Savoie, H., Chudasama, V., & Boyle, R. W. (2018). Synthesis of a novel HER2 targeted aza-BODIPY–antibody conjugate: synthesis, photophysical characterisation and in vitro evaluation. Organic & biomolecular chemistry, 16(7), (1144-1149). doi:10.1039/c7ob02957h. ISSN 1477-0520

We herein report the synthesis and analysis of a novel aza-BODIPY–antibody conjugate, formed by controlled and regioselective bioconjugation methodology. Employing the clinically relevant antibody, which targets HER2 positive cancers, represents the... Read More

Assembly of high-potency photosensitizer-antibody conjugates through application of dendron multiplier technology (2017)
Journal Article
Rodrigues, J. M., Cheng, M. H., Bryden, F., Maruani, A., Rodrigues, J. M. M., Cheng, M. H. Y., …Boyle, R. W. (2018). Assembly of high-potency photosensitizer-antibody conjugates through application of dendron multiplier technology. Bioconjugate chemistry, 29(1), 176-181. doi:10.1021/acs.bioconjchem.7b00678

© 2017 American Chemical Society. Exploitation of photosensitizers as payloads for antibody-based anticancer therapeutics offers a novel alternative to the small pool of commonly utilized cytotoxins. However, existing bioconjugation methodologies are... Read More

Generation of triplet excited states via photoinduced electron transfer in meso-anthra-BODIPY: fluorogenic response toward singlet oxygen in solution and in vitro (2017)
Journal Article
Filatov, M. A., Karuthedath, S., Polestshuk, P. M., Savoie, H., Flanagan, K. J., Sy, C., …Senge, M. O. (2017). Generation of triplet excited states via photoinduced electron transfer in meso-anthra-BODIPY: fluorogenic response toward singlet oxygen in solution and in vitro. Journal of the American Chemical Society, 139(18), (6282-6285). doi:10.1021/jacs.7b00551. ISSN 0002-7863

© 2017 American Chemical Society. Heavy atom-free BODIPY-anthracene dyads (BADs) generate locally excited triplet states by way of photoinduced electron transfer (PeT), followed by recombination of the resulting charge-separated states (CSS). Subsequ... Read More

Synthesis and bactericidal properties of porphyrins immobilized in a polyacrylamide support: influence of metal complexation on photoactivity (2017)
Journal Article
(2017). Synthesis and bactericidal properties of porphyrins immobilized in a polyacrylamide support: influence of metal complexation on photoactivity. Journal of materials chemistry B, Materials for biology and medicine /, 5(9), 1834-1845. doi:10.1039/c6tb03198f

Spectroscopic and photodynamic properties of three novel polymeric hydrogels bearing porphyrins have been studied in vitro on the recombinant bioluminescent Gram-negative Escherichia coli DH5α to assess their ability to inactivate bacterial strains i... Read More

NanoSOSG : a nanostructured fluorescent probe for detection of intracellular singlet oxygen (2017)
Journal Article
Ruiz-González, R., Gulías, Ò., Ruiz-González, R., Bresolí-Obach, R., Gulías, Ò., Agut, M., …Giuntini, F. (2017). NanoSOSG : a nanostructured fluorescent probe for detection of intracellular singlet oxygen. Angewandte Chemie, 56(11), (2885-2888). doi:10.1002/anie.201609050. ISSN 1433-7851

A biocompatible fluorescent nanoprobe for singlet oxygen (¹O₂) detection in biological systems was designed, synthesized, and characterized, that circumvents many of the limitations of the molecular probe Singlet Oxygen Sensor Green® (SOSG). This wid... Read More

Duramycin-porphyrin conjugates for targeting of tumour cells using photodynamic therapy (2016)
Journal Article
Broughton, L. J., Giuntini, F., Savoie, H., Bryden, F., Boyle, R. W., Maraveyas, A., & Madden, L. A. (2016). Duramycin-porphyrin conjugates for targeting of tumour cells using photodynamic therapy. Journal of photochemistry and photobiology. B, Biology, 163, (374-384). doi:10.1016/j.jphotobiol.2016.09.001. ISSN 1011-1344

Duramycin, through binding with phosphatidylethanolamine (PE), has been shown to be a selective molecular probe for the targeting and imaging of cancer cells. Photodynamic therapy aims to bring about specific cytotoxic damage to tumours through deliv... Read More

Synthesis, characterization and biological evaluation of a new photoactive hydrogel against Gram-positive and Gram-negative bacteria (2016)
Journal Article
Spagnul, C., Greenman, J., Wainwright, M., Kamil, Z., & Boyle, R. W. (2016). Synthesis, characterization and biological evaluation of a new photoactive hydrogel against Gram-positive and Gram-negative bacteria. Journal of Materials Chemistry B, 4(8), (1499-1509). doi:10.1039/c5tb02569a. ISSN 2050-7518

© 2016 The Royal Society of Chemistry. In 2013, the World Health Organization reported that 884 million people lack access to clean potable water. Photodynamic antimicrobial chemotherapy (PACT) is a very promising alternative to conventional antibiot... Read More

Mechanisms of growth inhibition of primary prostate epithelial cells following gamma irradiation or photodynamic therapy including senscence, necrosis, and autophagy, but not apoptosis (2015)
Journal Article
Frame, F. M., Savoie, H., Bryden, F., Giuntini, F., Mann, V. M., Simms, M. S., …Maitland, N. J. (2015). Mechanisms of growth inhibition of primary prostate epithelial cells following gamma irradiation or photodynamic therapy including senscence, necrosis, and autophagy, but not apoptosis. Cancer medicine, 5(1), (61-73). doi:10.1002/cam4.553. ISSN 2045-7634

In comparison to more differentiated cells, prostate cancer stem-like cells are radioresistant, which could explain radio-recurrent prostate cancer. Improvement of radiotherapeutic efficacy may therefore require combination therapy. We have investiga... Read More

Development of PDT/PET theranostics: synthesis and biological evaluation of an ¹⁸F-radiolabeled water soluble porphyrin (2015)
Journal Article
Entract, G. M., Bryden, F., Domarkas, J., Savoie, H., Allott, L., Archibald, S. J., …Boyle, R. W. (2015). Development of PDT/PET theranostics: synthesis and biological evaluation of an ¹⁸F-radiolabeled water soluble porphyrin. Molecular pharmaceutics, 12(12), 4414-4423. https://doi.org/10.1021/acs.molpharmaceut.5b00606

Synthesis of the first water-soluble porphyrin radiolabeled with fluorine-18 is described: a new molecular theranostic agent which integrates the therapeutic selectivity of photodynamic therapy (PDT) with the imaging efficacy of positron emission tom... Read More

Site-selective multi-porphyrin attachment enables the formation of a next-generation antibody-based photodynamic therapeutic (2015)
Journal Article
Maruani, A., Savoie, H., Bryden, F., Caddick, S., Boyle, R., & Chudasama, V. (2015). Site-selective multi-porphyrin attachment enables the formation of a next-generation antibody-based photodynamic therapeutic. Chemical communications : Chem comm / the Royal Society of Chemistry, 51(83), 15304-15307. https://doi.org/10.1039/c5cc06985h

Herein we present a significant step towards next-generation antibody-based photodynamic therapeutics. Site-selective modification of a clinically relevant monoclonal antibody, with a serum-stable linker bearing a strained alkyne, allows for the cont... Read More

Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles (2015)
Journal Article
Aylott, J. W., Chan, W. C., Jones, D. R. E., Giuntini, F., Chauhan, V. M., Lavado, A. S., …Zen, A. A. (2015). Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles. Nanoscale, 7(34), 14525-14531. doi:10.1039/c5nr00795j

Nanoparticles capable of generating controlled amounts of intracellular reactive oxygen species (ROS), that advance the study of oxidative stress and cellular communication, were synthesized by functionalizing polyacrylamide nanoparticles with zinc(I... Read More

Immobilized photosensitizers for antimicrobial applications (2015)
Journal Article
Spagnul, C., Turner, L. C., & Boyle, R. W. (2015). Immobilized photosensitizers for antimicrobial applications. Journal of photochemistry and photobiology. B, Biology, 150(September), 11-30. https://doi.org/10.1016/j.jphotobiol.2015.04.021

Photodynamic antimicrobial chemotherapy (PACT) is a very promising alternative to conventional antibiotics for the efficient inactivation of pathogenic microorganisms; this is due to the fact that it is virtually impossible for resistant strains to d... Read More

PET/PDT theranostics: Synthesis and biological evaluation of a peptide-targeted gallium porphyrin (2015)
Journal Article
Bryden, F., Boyle, R. W., Savoie, H., & Rosca, E. V. (2015). PET/PDT theranostics: Synthesis and biological evaluation of a peptide-targeted gallium porphyrin. Dalton transactions : an international journal of inorganic chemistry / RSoC, 44(11), (4925-4932). doi:10.1039/c4dt02949f. ISSN 1477-9226

The development of novel theranostic agents is an important step in the pathway towards personalised medicine, with the combination of diagnostic and therapeutic modalities into a single treatment agent naturally lending itself to the optimisation an... Read More

Conjugatable water-soluble Pt(ii) and Pd(ii) porphyrin complexes: Novel nano- and molecular probes for optical oxygen tension measurement in tissue engineering (2014)
Journal Article
Giuntini, F., Aylott, J. W., Boyle, R. W., Athanasiadis, A., Beeby, A., Brown, R. A., …Rosser, G. A. (2014). Conjugatable water-soluble Pt(ii) and Pd(ii) porphyrin complexes: Novel nano- and molecular probes for optical oxygen tension measurement in tissue engineering. Photochemical & photobiological sciences : an international journal, 13(7), (1039-1051). doi:10.1039/c4pp00026a. ISSN 1474-905X

Measurement of oxygen tension in compressed collagen sheets was performed using matrix-embedded optical oxygen sensors based on platinum(II) and palladium(II) porphyrins supported on polyacrylamide nanoparticles. Bespoke, fully water-soluble, mono-fu... Read More

Regioselective and stoichiometrically controlled conjugation of photodynamic sensitizers to a HER2 targeting antibody fragment (2014)
Journal Article
Bryden, F., Boyle, R. W., Savoie, H., Caddick, S., Chudasama, V., Maruani, A., & Smith, M. E. B. (2014). Regioselective and stoichiometrically controlled conjugation of photodynamic sensitizers to a HER2 targeting antibody fragment. Bioconjugate chemistry, 25(3), 611-617. doi:10.1021/bc5000324

The rapidly increasing interest in the synthesis of antibody–drug conjugates as powerful targeted anticancer agents demonstrates the growing appreciation of the power of antibodies and antibody fragments as highly selective targeting moieties. This t... Read More

Huisgen-based conjugation of water-soluble porphyrins to deprotected sugars: Towards mild strategies for the labelling of glycans (2014)
Journal Article
Giuntini, F., Bryden, F., Boyle, R. W., Daly, R., & Scanlan, E. M. (2014). Huisgen-based conjugation of water-soluble porphyrins to deprotected sugars: Towards mild strategies for the labelling of glycans. Organic & biomolecular chemistry, 12(8), (1203-1206). doi:10.1039/c3ob42306a. ISSN 1477-0520

Fully deprotected alkynyl-functionalised mono- and oligosaccharides undergo CuAAC-based conjugation with water-soluble porphyrin azides in aqueous environments. The mild reaction conditions are fully compatible with the presence of labile glycosidic... Read More