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Induction of endothelial cell proliferation by recombinant and microparticle-tissue factor involves β1-integrin and extracellular signal regulated kinase activation (2010)
Journal Article
Collier, M. E. W., & Ettelaie, C. (2010). Induction of endothelial cell proliferation by recombinant and microparticle-tissue factor involves β1-integrin and extracellular signal regulated kinase activation. Arteriosclerosis, Thrombosis, and Vascular Biology, 30(9), 1810-1817. https://doi.org/10.1161/ATVBAHA.110.211854

Objective: Increased levels of circulating tissue factor (TF) in the form of microparticles increase the risk of thrombosis. However, any direct influence of microparticle-associated TF on vascular endothelial cell proliferation is not known. In this... Read More about Induction of endothelial cell proliferation by recombinant and microparticle-tissue factor involves β1-integrin and extracellular signal regulated kinase activation.

Synthesis, structure and reactivity of 1-(4-nitrobenzyl)-2-chloromethyl benzimidazole (2010)
Journal Article
Sparke, A. E., Fisher, C. M., Mewis, R. E., & Archibald, S. J. (2010). Synthesis, structure and reactivity of 1-(4-nitrobenzyl)-2-chloromethyl benzimidazole. Tetrahedron letters; the international organ for the rapid publication of preliminary communications in organic chemistry, 51(36), 4723-4726. https://doi.org/10.1016/j.tetlet.2010.07.010

The synthesis of 1-(4-nitrobenzyl)-2-chloromethyl benzimidazole, which undergoes a nucleophilic substitution with pyridine in the absence of additional base, is reported. The key steps are the reaction of 1,2-phenylenediamine to give exclusively the... Read More about Synthesis, structure and reactivity of 1-(4-nitrobenzyl)-2-chloromethyl benzimidazole.

Exposure to acute hypoxia induces a transient DNA damage response which includes Chk1 and TLK1 (2010)
Journal Article
Pires, I. M., Bencokova, Z., Hammond, E. M., & McGurk, C. (2010). Exposure to acute hypoxia induces a transient DNA damage response which includes Chk1 and TLK1. Cell cycle, 9(13), 2502-2507. https://doi.org/10.4161/cc.9.13.12059

Severe hypoxia has been demonstrated to induce a replication arrest which is associated with decreased levels of nucleotides. Chk1 is rapidly phosphorylated in response to severe hypoxia and in turn deactivates TLK1 through phosphorylation. Loss of C... Read More about Exposure to acute hypoxia induces a transient DNA damage response which includes Chk1 and TLK1.

Myostatin knockout mice increase oxidative muscle phenotype as an adaptive response to exercise (2010)
Journal Article
Matsakas, A., Mouisel, E., Amthor, H., & Patel, K. (2010). Myostatin knockout mice increase oxidative muscle phenotype as an adaptive response to exercise. Journal of Muscle Research and Cell Motility, 31(2), 111-125. https://doi.org/10.1007/s10974-010-9214-9

Myostatin-deficient mice (MSTN (-/-)) display excessive muscle mass and this is associated with a profound loss of oxidative metabolic properties. In this study we analysed the effect of two endurance-based exercise regimes, either a forced high-impa... Read More about Myostatin knockout mice increase oxidative muscle phenotype as an adaptive response to exercise.

A cellular screening assay using analysis of metal-modified fluorescence lifetime (2010)
Journal Article
Cade, N. I., Fruhwirth, G., Archibald, S. J., Ng, T., & Richards, D. (2010). A cellular screening assay using analysis of metal-modified fluorescence lifetime. Biophysical journal, 98(11), 2752-2757. https://doi.org/10.1016/j.bpj.2010.03.016

Current methods for screening cell receptor internalization often require complex image analysis with limited sensitivity. Here we describe a novel bioassay based on detection of changes in global fluorescence lifetime above a gold substrate, with su... Read More about A cellular screening assay using analysis of metal-modified fluorescence lifetime.

Regulation of autophagy by ATF4 in response to severe hypoxia (2010)
Journal Article
Rzymski, T., Milani, M., Pike, L., Buffa, F., Mellor, H. R., Winchester, L., Pires, I., Hammond, E., Ragoussis, I., & Harris, A. L. (2010). Regulation of autophagy by ATF4 in response to severe hypoxia. Oncogene, 29(31), 4424-4435. https://doi.org/10.1038/onc.2010.191

Activating transcription factor 4 (ATF4) is a transcription factor induced under severe hypoxia and a component of the PERK pathway involved in the unfolded protein response (UPR), a process that protects cells from the negative consequences of endop... Read More about Regulation of autophagy by ATF4 in response to severe hypoxia.

Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling (2010)
Journal Article
Shaw, K. J., Docker, P. T., Yelland, J. V., Dyer, C. E., Greenman, J., Greenway, G. M., & Haswell, S. J. (2010). Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling. Lab on a chip, 10(13), 1725-1728. https://doi.org/10.1039/c000357n

A microwave heating system is described for performing polymerase chain reaction (PCR) in a microfluidic device. The heating system, in combination with air impingement cooling, provided rapid thermal cycling with heating and cooling rates of up to 6... Read More about Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling.

A hypoplastic model of skeletal muscle development displaying reduced foetal myoblast cell numbers, increased oxidative myofibres and improved specific tension capacity (2010)
Journal Article
Otto, A., Macharia, R., Matsakas, A., Valasek, P., Mankoo, B. S., & Patel, K. (2010). A hypoplastic model of skeletal muscle development displaying reduced foetal myoblast cell numbers, increased oxidative myofibres and improved specific tension capacity. Developmental Biology, 343(1-2), 51-62. https://doi.org/10.1016/j.ydbio.2010.04.014

The major component of skeletal muscle is the myofibre. Genetic intervention inducing over-enlargement of myofibres beyond a certain threshold through acellular growth causes a reduction in the specific tension generating capacity of the muscle. Howe... Read More about A hypoplastic model of skeletal muscle development displaying reduced foetal myoblast cell numbers, increased oxidative myofibres and improved specific tension capacity.

Variation in amino acid and lipid composition of latent fingerprints (2010)
Journal Article
Croxton, R. S., Baron, M. G., Butler, D., Kent, T., & Sears, V. G. (2010). Variation in amino acid and lipid composition of latent fingerprints. Forensic Science International, 199(1-3), 93-102. https://doi.org/10.1016/j.forsciint.2010.03.019

The enhancement of latent fingerprints, both at the crime scene and in the laboratory using an array of chemical, physical and optical techniques, permits their use for identification. Despite the plethora of techniques available, there are occasions... Read More about Variation in amino acid and lipid composition of latent fingerprints.

Genetics and cardiac channelopathies (2010)
Journal Article
Campuzano, O., Beltrán-Álvarez, P., Iglesias, A., Scornik, F., Pérez, G., & Brugada, R. (2010). Genetics and cardiac channelopathies. Genetics in Medicine, 12(5), 260-267. https://doi.org/10.1097/GIM.0b013e3181d81636

Sudden cardiac death is a major contributor to mortality in industrialized nations; in fact, it is the cause of more deaths than acquired immune deficiency syndrome, lung and breast cancer, and stroke together. Frequently, the autopsy becomes the pri... Read More about Genetics and cardiac channelopathies.

The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flow (2010)
Journal Article
Ngamsom, B., Hickey, A. M., Greenway, G. M., Littlechild, J. A., McCreedy, T., Watts, P., & Wiles, C. (2010). The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flow. Organic & biomolecular chemistry, 8(10), 2419-2424. https://doi.org/10.1039/b924100k

Through the preparation of a novel controlled pore glass-poly(pyrrole) material we have developed a conducting support that is not only suitable for the co-immobilisation of enzymes and co-factors, but also enables the facile electrochemical regenera... Read More about The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flow.

Biological and clinical implications of nicastrin expression in invasive breast cancer (2010)
Journal Article
Filipović, A., Gronau, J. H., Green, A. R., Wang, J., Vallath, S., Shao, D., Rasul, S., Ellis, I. O., Yagüe, E., Sturge, J., & Coombes, R. C. (2011). Biological and clinical implications of nicastrin expression in invasive breast cancer. Breast cancer research and treatment, 125(1), 43-53. https://doi.org/10.1007/s10549-010-0823-1

Nicastrin is an essential component of the gamma secretase (GS) enzyme complex, required for its synthesis and recognition of substrates for proteolytic cleavage. The purpose of this study was to investigate whether nicastrin has prognostic value or... Read More about Biological and clinical implications of nicastrin expression in invasive breast cancer.

Biomedical applications of macrocyclic ligand complexes (2010)
Journal Article
Mewis, R. E., & Archibald, S. J. (2010). Biomedical applications of macrocyclic ligand complexes. Coordination chemistry reviews, 254(15-16), 1686-1712. https://doi.org/10.1016/j.ccr.2010.02.025

Macrocyclic chelators can form highly stable complexes with transition metals and lanthanides. In this review, the recent advances towards biomedical applications of macrocyclic complexes are outlined. The use of such complexes in imaging as MRI cont... Read More about Biomedical applications of macrocyclic ligand complexes.

Biomarkers of chemotherapy resistance in breast cancer identified by proteomics: current status (2010)
Journal Article
Hodgkinson, V. C., Eagle, G. L., Drew, P. J., Lind, M. J., & Cawkwell, L. (2010). Biomarkers of chemotherapy resistance in breast cancer identified by proteomics: current status. Cancer Letters, 294(1), 13-24. https://doi.org/10.1016/j.canlet.2010.01.036

This review describes and discusses the advantages and limitations of proteomic approaches in the identification of biomarkers associated with chemotherapy resistance. Both gel-based (two-dimensional polyacrylamide gel electrophoresis) and gel-free (... Read More about Biomarkers of chemotherapy resistance in breast cancer identified by proteomics: current status.

An internal sequence targets Trypanosoma brucei triosephosphate isomerase to glycosomes (2010)
Journal Article
Galland, N., de Walque, S., Voncken, F. G., Verlinde, C. L., & Michels, P. A. (2010). An internal sequence targets Trypanosoma brucei triosephosphate isomerase to glycosomes. Molecular and Biochemical Parasitology, 171(1), 45-49. https://doi.org/10.1016/j.molbiopara.2010.01.002

In kinetoplastid protists, glycolysis is compartmentalized in glycosomes, organelles belonging to the peroxisome family. The Trypanosoma brucei glycosomal enzyme triosephosphate isomerase (TPI) does not contain either of the two established peroxisom... Read More about An internal sequence targets Trypanosoma brucei triosephosphate isomerase to glycosomes.

Gender-based issues in interventional cardiology: A consensus statement from the Women in Innovations (WIN) Initiative (2010)
Journal Article
Chieffo, A., Hoye, A., Mauri, F., Mikhail, G. W., Ammerer, M., Grines, C., Grinfeld, L., Madan, M., Presbitero, P., Skelding, K. A., Weiner, B. H., Mehran, R., & on behalf of the WIN Group. (2010). Gender-based issues in interventional cardiology: A consensus statement from the Women in Innovations (WIN) Initiative. Catheterization and Cardiovascular Interventions, 75(2), 145-152. https://doi.org/10.1002/ccd.22327

Cardiovascular disease (CVD) is the leading cause of mortality in women, yet studies have suggested that it is often under-recognized. Of particular concern is the apparent suboptimal treatment of women in comparison to men, with less revascularizati... Read More about Gender-based issues in interventional cardiology: A consensus statement from the Women in Innovations (WIN) Initiative.

A systematic screen for proteing-lipid interactions in Saccharomyces cerevisiae (2010)
Journal Article
Gallego, O., Betts, M. J., Gvozdenovic-Jeremic, J., Maeda, K., Matetzki, C., Aguilar-Gurrieri, C., Beltran-Alvarez, P., Bonn, S., Fernández-Tornero, C., Jensen, L. J., Kuhn, M., Trott, J., Rybin, V., Müller, C. W., Bork, P., Kaksonen, M., Russell, R. B., & Gavin, A. C. (2010). A systematic screen for proteing-lipid interactions in Saccharomyces cerevisiae. Molecular Systems Biology, 6(1), Article 430. https://doi.org/10.1038/msb.2010.87

Proteing-metabolite networks are central to biological systems, but are incompletely understood. Here, we report a screen to catalog proteing-lipid interactions in yeast. We used arrays of 56 metabolites to measure lipid-binding fingerprints of 172 p... Read More about A systematic screen for proteing-lipid interactions in Saccharomyces cerevisiae.

Oxaliplatin responses in colorectal cancer cells are modulated by CHK2 kinase inhibitors (2010)
Journal Article
Pires, I. M., Ward, T. H., & Dive, C. (2010). Oxaliplatin responses in colorectal cancer cells are modulated by CHK2 kinase inhibitors. British Journal of Pharmacology, 159(6), 1326-1338. https://doi.org/10.1111/j.1476-5381.2009.00607.x

Background and purpose: Checkpoint kinase 2 (CHK2) is activated by DNA damage and can contribute to p53 stabilization, modulating growth arrest and/or apoptosis. We investigated the contribution of CHK2 to oxaliplatin-mediated toxicity in a colorecta... Read More about Oxaliplatin responses in colorectal cancer cells are modulated by CHK2 kinase inhibitors.

Effects of acute versus chronic hypoxia on DNA damage responses and genomic instability (2010)
Journal Article
Pires, I. M., Bencokova, Z., Milani, M., Folkes, L. K., Li, J.-L., Stratford, M. R., Harris, A. L., & Hammond, E. M. (2010). Effects of acute versus chronic hypoxia on DNA damage responses and genomic instability. Cancer Research, 70(3), 925-935. https://doi.org/10.1158/0008-5472.CAN-09-2715

Questions exist concerning the effects of acute versus chronic hypoxic conditions on DNA replication and genomic stability that may influence tumorigenesis. Severe hypoxia causes replication arrest independent of S-phase checkpoint, DNA damage respon... Read More about Effects of acute versus chronic hypoxia on DNA damage responses and genomic instability.