All Outputs (918)

Development of a Microfluidic Culture Paradigm for Ex Vivo Maintenance of Human Glioblastoma Tissue: A New Glioblastoma Model? (2019)
Journal Article
Olubajo, F., Achawal, S., & Greenman, J. (2020). Development of a Microfluidic Culture Paradigm for Ex Vivo Maintenance of Human Glioblastoma Tissue: A New Glioblastoma Model?. Translational Oncology, 13(1), 1-10. https://doi.org/10.1016/j.tranon.2019.09.002

© 2019 The Authors BACKGROUND: One way to overcome the genetic and molecular variations within glioblastoma is to treat each tumour on an individual basis. To facilitate this, we have developed a microfluidic culture paradigm that maintains human gli... Read More about Development of a Microfluidic Culture Paradigm for Ex Vivo Maintenance of Human Glioblastoma Tissue: A New Glioblastoma Model?.

Accumulation of tissue factor in endothelial cells promotes cellular apoptosis through over-activation of Src1 and involves β1-integrin signalling (2019)
Journal Article
Ethaeb, A. M., Mohammad, M. A., Madkhali, Y., Maraveyas, A., Featherby, S., Greenman, J., & Ettelaie, C. (2020). Accumulation of tissue factor in endothelial cells promotes cellular apoptosis through over-activation of Src1 and involves β1-integrin signalling. Apoptosis, 25(1-2), 29-41. https://doi.org/10.1007/s10495-019-01576-2

Accumulation of tissue factor (TF) within cells leads to cellular apoptosis mediated through p38 and p53 pathways. In this study, the involvement of Src1 in the induction of TF-mediated cell apoptosis, and the mechanisms of Src1 activation were inves... Read More about Accumulation of tissue factor in endothelial cells promotes cellular apoptosis through over-activation of Src1 and involves β1-integrin signalling.

Transcriptomic Adjustments of Staphylococcus aureus COL (MRSA) Forming Biofilms Under Acidic and Alkaline Conditions (2019)
Journal Article
Efthimiou, G., Tsiamis, G., Typas, M., & Pappas, K. (2019). Transcriptomic Adjustments of Staphylococcus aureus COL (MRSA) Forming Biofilms Under Acidic and Alkaline Conditions. Frontiers in Microbiology, 10, Article 02393. https://doi.org/10.3389/fmicb.2019.02393

© Copyright © 2019 Efthimiou, Tsiamis, Typas and Pappas. Methicillin-resistant Staphylococcus aureus (MRSA) strains are important human pathogens and a significant health hazard for hospitals and the food industry. They are resistant to β-lactam anti... Read More about Transcriptomic Adjustments of Staphylococcus aureus COL (MRSA) Forming Biofilms Under Acidic and Alkaline Conditions.

Optimising platelet secretomes to deliver robust tissue-specific regeneration (2019)
Journal Article
Scully, D., Sfyri, P., Wilkinson, H. N., Acebes‐Huerta, A., Verpoorten, S., Muñoz‐Turrillas, M. C., Parnell, A., Patel, K., Hardman, M. J., Gutiérrez, L., & Matsakas, A. (2020). Optimising platelet secretomes to deliver robust tissue-specific regeneration. Journal of tissue engineering and regenerative medicine, 14(1), 82-98. https://doi.org/10.1002/term.2965

Promoting cell proliferation is the cornerstone of most tissue regeneration therapies. As platelet-based applications promote cell division and can be customised for tissue-specific efficacy, this makes them strong candidates for developing novel reg... Read More about Optimising platelet secretomes to deliver robust tissue-specific regeneration.

Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya (2019)
Journal Article
Ngamsom, B., Wandera, E. A., Iles, A., Kimani, R., Muregi, F., Gitaka, J., & Pamme, N. (2019). Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya. Analyst, 144(23), 6889-6897. https://doi.org/10.1039/c9an01808e

© The Royal Society of Chemistry 2019. We report the rapid detection (20 min) of Streptococcus agalactiae, Group B Streptococcus (GBS) employing on-chip magnetic isolation of GBS based on immiscible filtration assisted by surface tension (IFAST), fol... Read More about Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya.

Loss of CD36 protects against diet-induced obesity but results in impaired muscle stem cell function, delayed muscle regeneration and hepatic steatosis (2019)
Journal Article
Verpoorten, S., Sfyri, P., Scully, D., Mitchell, R., Tzimou, A., Mougios, V., Patel, K., & Matsakas, A. (2020). Loss of CD36 protects against diet-induced obesity but results in impaired muscle stem cell function, delayed muscle regeneration and hepatic steatosis. Acta Physiologica, 228(3), https://doi.org/10.1111/apha.13395

Aim: The prevalence of obesity is a major risk factor for cardiovascular and metabolic diseases including impaired skeletal muscle regeneration. Since skeletal muscle regenerative capacity is regulated by satellite cells, we aimed to investigate whet... Read More about Loss of CD36 protects against diet-induced obesity but results in impaired muscle stem cell function, delayed muscle regeneration and hepatic steatosis.

Retrospective Medical Record Research: Reflections of A Unsponsored Researcher (2019)
Journal Article
Bature, F., Guinn, B., Vseteckova, J., Pang, D., & Pappas, Y. (2019). Retrospective Medical Record Research: Reflections of A Unsponsored Researcher. Biomedical Journal of Scientific & Technical Research, 21(4), 16080-16086. https://doi.org/10.26717/BJSTR.2019.21.003642

This article is a reflective narrative by the author following the successful completion of a doctoral thesis involving the analysis of primary care data. The aim of the study was to identify patterns in signs and symptoms preceding a clinical diagno... Read More about Retrospective Medical Record Research: Reflections of A Unsponsored Researcher.

TRPC6 Binds to and Activates Calpain, Independent of Its Channel Activity, and Regulates Podocyte Cytoskeleton, Cell Adhesion, and Motility (2019)
Journal Article
Farmer, L., Rollason, R., Whitcomb, D., Ni, L., Goodlif, A., Lay, A., Birnbaumer, L., Heesom, K., Xu, S.-Z., Saleem, M., & Welsh, G. (2019). TRPC6 Binds to and Activates Calpain, Independent of Its Channel Activity, and Regulates Podocyte Cytoskeleton, Cell Adhesion, and Motility. Journal of the American Society of Nephrology : JASN, 30(10), 1910-1924. https://doi.org/10.1681/ASN.2018070729

BACKGROUND: Mutations in the transient receptor potential channel 6 (TRPC6) gene are associated with an inherited form of FSGS. Despite widespread expression, patients with TRPC6 mutations do not present with any other pathologic phenotype, suggesti... Read More about TRPC6 Binds to and Activates Calpain, Independent of Its Channel Activity, and Regulates Podocyte Cytoskeleton, Cell Adhesion, and Motility.

Next-generation sequencing for the diagnosis of MYH9-RD: Predicting pathogenic variants (2019)
Journal Article
Bury, L., Megy, K., Stephens, J. C., Grassi, L., Greene, D., Gleadall, N., Althaus, K., Allsup, D., Bariana, T. K., Bonduel, M., Butta, N. V., Collins, P., Curry, N., Deevi, S. V., Downes, K., Duarte, D., Elliott, K., Falcinelli, E., Furie, B., Keeling, D., …Westbury, S. K. (2020). Next-generation sequencing for the diagnosis of MYH9-RD: Predicting pathogenic variants. Human Mutation, 41(1), 277-290. https://doi.org/10.1002/humu.23927

Human Mutation published by Wiley Periodicals, Inc. The heterogeneous manifestations of MYH9-related disorder (MYH9-RD), characterized by macrothrombocytopenia, Döhle-like inclusion bodies in leukocytes, bleeding of variable severity with, in some ca... Read More about Next-generation sequencing for the diagnosis of MYH9-RD: Predicting pathogenic variants.

Characterisation of a mitochondrial iron transporter of the pathogen Trypanosoma brucei (2019)
Journal Article
Zheng, F., Colasante, C., & Voncken, F. (2019). Characterisation of a mitochondrial iron transporter of the pathogen Trypanosoma brucei. Molecular and Biochemical Parasitology, 233, Article 111221. https://doi.org/10.1016/j.molbiopara.2019.111221

Similar to higher eukaryotes, the protist parasite T. brucei harbours several iron-containing proteins that regulate DNA and protein processing, oxidative stress defence and mitochondrial respiration. The synthesis of these proteins occurs either in... Read More about Characterisation of a mitochondrial iron transporter of the pathogen Trypanosoma brucei.

The effect of radioiodine treatment on the diseased thyroid gland (2019)
Journal Article
Riley, A. S., McKenzie, G. A. G., Green, V., Schettino, G., England, R. J. A., & Greenman, J. (2019). The effect of radioiodine treatment on the diseased thyroid gland. International Journal of Radiation Biology, 95(12), 1718-1727. https://doi.org/10.1080/09553002.2019.1665206

Purpose: Radioiodine (I131) therapy is the treatment mainstay for several benign and malignant thyroid disorders, however I131 is known to cause DNA damage and liberation of thyroidal self-antigens inducing secondary immunoreactivity. The exact mecha... Read More about The effect of radioiodine treatment on the diseased thyroid gland.

Effect of home-based resistance training performed with or without a high-speed component in adults with severe obesity (2019)
Journal Article
Orange, S. T., Marshall, P., Madden, L. A., & Vince, R. V. (2020). Effect of home-based resistance training performed with or without a high-speed component in adults with severe obesity. Translational Sports Medicine, 3(1), 34-45. https://doi.org/10.1002/tsm2.115

The objectives of this study were to:(a) evaluate the effects of walking and home-based resistance training on function, strength, power, anthropometry, and quality of life (QoL) in adults with severe obesity, and (b) assess whether performing resist... Read More about Effect of home-based resistance training performed with or without a high-speed component in adults with severe obesity.

Notch signalling is a potential resistance mechanism of progenitor cells within patient‐derived prostate cultures following ROS‐inducing treatments (2019)
Journal Article
Packer, J. R., Hirst, A. M., Droop, A. P., Adamson, R., Simms, M. S., Mann, V. M., Frame, F. M., O'Connell, D., & Maitland, N. J. (2019). Notch signalling is a potential resistance mechanism of progenitor cells within patient‐derived prostate cultures following ROS‐inducing treatments. FEBS Letters, https://doi.org/10.1002/1873-3468.13589

Low Temperature Plasma (LTP) generates reactive oxygen and nitrogen species, causing cell death, similarly to radiation. Radiation resistance results in tumour recurrence, however mechanisms of LTP resistance are unknown. LTP was applied to patient‐d... Read More about Notch signalling is a potential resistance mechanism of progenitor cells within patient‐derived prostate cultures following ROS‐inducing treatments.

The CD105:CD106 microparticle ratio is CD106 dominant in polycystic ovary syndrome compared to type 2 diabetes and healthy subjects (2019)
Journal Article
Al-Qaissi, A., Alqarni, S., Javed, Z., Atkin, S. L., Sathyapalan, T., Vince, R., & Madden, L. A. (2019). The CD105:CD106 microparticle ratio is CD106 dominant in polycystic ovary syndrome compared to type 2 diabetes and healthy subjects. Endocrine, https://doi.org/10.1007/s12020-019-02059-9

© 2019, The Author(s). Background: A retrospective analysis was carried out from patients and controls during the past 5 years from a series of studies investigating endothelial microparticles (MP). Methods: In total, 319 samples from 207 individuals... Read More about The CD105:CD106 microparticle ratio is CD106 dominant in polycystic ovary syndrome compared to type 2 diabetes and healthy subjects.

Tissue iron promotes wound repair via M2 macrophage polarisation and the chemokines CCL17 and CCL22 (2019)
Journal Article
Wilkinson, H. N., Roberts, E. R., Stafford, A. R., Banyard, K. L., Matteucci, P., Mace, K. A., & Hardman, M. J. (2019). Tissue iron promotes wound repair via M2 macrophage polarisation and the chemokines CCL17 and CCL22. American Journal of Pathology, 189(11), 2196-2208. https://doi.org/10.1016/j.ajpath.2019.07.015

Macrophages are important for effective iron recycling and erythropoiesis, but they also play a crucial role in wound healing, orchestrating tissue repair. Recently, we demonstrated a significant accumulation of iron in healing wounds and a requireme... Read More about Tissue iron promotes wound repair via M2 macrophage polarisation and the chemokines CCL17 and CCL22.

Exercise training as a mediator for enhancing coronary collateral circulation: A review of the evidence (2019)
Journal Article
Nickolay, T., Nichols, S., Ingle, L., & Hoye, A. (2020). Exercise training as a mediator for enhancing coronary collateral circulation: A review of the evidence. Current Cardiology Reviews, 16(3), 212-220. https://doi.org/10.2174/1573403X15666190819144336

Coronary collateral vessels supply blood to areas of myocardium at risk after arterial occlusion. Flow through these channels is driven by a pressure gradient between the donor and the occluded artery. Concomitant with increased collateral flow is an... Read More about Exercise training as a mediator for enhancing coronary collateral circulation: A review of the evidence.

Mibefradil, a T-type Ca2+ channel blocker also blocks Orai channels by action at the extracellular surface (2019)
Journal Article
Li, P., Rubaiy, H. N., Chen, G.-L., Hallett, T., Zaibi, N., Zeng, B., Saurabh, R., & Xu, S.-Z. (2019). Mibefradil, a T-type Ca2+ channel blocker also blocks Orai channels by action at the extracellular surface. British Journal of Pharmacology, 176, 3845–3856. https://doi.org/10.1111/bph.14788

Background and purpose Mibefradil (Mib), a T‐type Ca2+ channel blocker, has been investigated for treating solid tumours. However, its underlying mechanisms are still unclear. Here we aimed to investigate the pharmacological aspect of Mib on ORAI st... Read More about Mibefradil, a T-type Ca2+ channel blocker also blocks Orai channels by action at the extracellular surface.

Long-term ambient air-stable cubic CsPbBr3 perovskite quantum dots using molecular bromine (2019)
Journal Article
Thapa, S., Bhardwaj, K., Basel, S., Pradhan, S., Eling, C. J., Adawi, A. M., Bouillard, J. S. G., Stasiuk, G. J., Reiss, P., Pariyar, A., & Tamang, S. (2019). Long-term ambient air-stable cubic CsPbBr3 perovskite quantum dots using molecular bromine. Nanoscale advances, 1(9), 3388-3391. https://doi.org/10.1039/c9na00486f

We report unprecedented phase stability of cubic CsPbBr3 quantum dots in ambient air obtained by using Br2 as halide precursor. Mechanistic investigation reveals the decisive role of temperature-controlled in situ generated, oleylammonium halide spec... Read More about Long-term ambient air-stable cubic CsPbBr3 perovskite quantum dots using molecular bromine.

Side-bridged cyclam transition metal complexes bearing a phenolic ether or a phenolate pendent arm (2019)
Journal Article
Mewis, R. E., & Archibald, S. J. (2019). Side-bridged cyclam transition metal complexes bearing a phenolic ether or a phenolate pendent arm. Polyhedron, 171, 578-589. https://doi.org/10.1016/j.poly.2019.08.003

Side-bridged cyclam transition metal complexes (M = Ni(II), Cu(II) and Zn(II)) bearing a phenolic ether or phenolate pendent arm have been synthesised. For [NiL1]+ and [CuL1]+, evidence for a phenoxyl radical was obtained (quasi reversible peak at +0... Read More about Side-bridged cyclam transition metal complexes bearing a phenolic ether or a phenolate pendent arm.

A bioinformatics toolkit: in silico tools and online resources for investigating genetic variation (2019)
Journal Article
Webster, S. J., Aldossary, M. A., & Hampshire, D. J. (2019). A bioinformatics toolkit: in silico tools and online resources for investigating genetic variation. Seminars in Thrombosis and Hemostasis, 45(7), 674-684. https://doi.org/10.1055/s-0039-1692978

With the advent of large-scale next-generation sequencing initiatives, there is an increasing importance to interpret and understand the potential phenotypic influence of identified genetic variation and its significance in the human genome. Bioinfor... Read More about A bioinformatics toolkit: in silico tools and online resources for investigating genetic variation.