Repeated supra-maximal sprint cycling with and without sodium bicarbonate supplementation induces endothelial microparticle release

Kirk, Richard J.; Peart, Daniel J.; Madden, Leigh A.; Vince, Rebecca V.

Abstract

Under normal homeostatic conditions, the endothelium releases microparticles (MP), which are known to increase under stressful conditions and in disease states. CD105 (endoglin) and CD106 (vascular cell adhesion molecule-1) are expressed on the surface of endothelial cells and increased expression in response to stress may be observed. A randomised-controlled double-blinded study aimed to examine the use of endothelial microparticles as a marker for the state of one’s endothelium, as well as whether maintaining acid-base homeostasis affects the release of these MP. This study tested seven healthy male volunteers, who completed a strenuous cycling protocol, with venous blood analysed for CD105+ and CD106+ MP by flow cytometry at regular intervals. Prior to each trial participants consumed either 0.3 g·kg-1 body mass of sodium bicarbonate (NaHCO3), or 0.045 g·kg-1 body mass of sodium chloride (NaCl). A significant rise in endothelial CD105+MP and CD106+MP (p < 0.05) was observed at 90 minutes post exercise. A significant trend was shown for these MP to return to resting levels 180 minutes post exercise in both groups. No significance was found between experimental groups, suggesting that maintaining acid-base variables closer to basal levels has little effect upon the endothelial stress response for this particular exercise mode. In conclusion, strenuous exercise is accompanied by MP release and the endothelium is able to rapidly recover in healthy individuals, whilst maintaining acid-base homeostasis does not attenuate the MP release from the endothelium after exercise.

Citation

Kirk, R. J., Peart, D. J., Madden, L. A., & Vince, R. V. (2014). Repeated supra-maximal sprint cycling with and without sodium bicarbonate supplementation induces endothelial microparticle release. European journal of sport science : the official journal of the European College of Sport Science, 14(4), 345-352. https://doi.org/10.1080/17461391.2013.785600

• Journal Article Type: Article
• Online Publication Date: May 17, 2013
• Publication Date: May 19, 2014
• Deposit Date: Jun 9, 2015
• Publicly Available Date: Jun 9, 2015
• Journal: European journal of sport science
• Print ISSN: 1746-1391
• Electronic ISSN: 1536-7290
• Publisher: Taylor and Francis
• Peer Reviewed: Peer Reviewed
• Volume: 14
• Issue: 4
• Pages: 345-352
• DOI: https://doi.org/10.1080/17461391.2013.785600
• Keywords: Endothelial function; Microparticles; Anaerobic exercise; Sodium bicarbonate; Vascular cell adhesion molecule-1
• Public URL: https://hull-repository.worktribe.com/output/374914
• Publisher URL: https://www.tandfonline.com/doi/abs/10.1080/17461391.2013.785600
• Additional Information: This is an Accepted Manuscript of an article published by Taylor & Francis in European journal of sport science on 17th May 2013, available online: http://wwww.tandfonline.com/10.1080/17461391.2013.785600