All Outputs (11)

Investigating oxygen transport efficiencies in precision-cut liver slice-based organ-on-a-chip devices (2021)
Journal Article
Christensen, M. G., Cawthorne, C., Dyer, C. E., Greenman, J., & Pamme, N. (2021). Investigating oxygen transport efficiencies in precision-cut liver slice-based organ-on-a-chip devices. Microfluidics and Nanofluidics, 25(4), Article 35. https://doi.org/10.1007/s10404-021-02434-x

Microfluidic ‘organ-on-a-chip’ devices hold great potential for better mimicking the continuous flow microenvironment experienced by tissue and cells in vivo, thereby ensuring realistic transport of nutrients and elimination of waste products. Howeve... Read More about Investigating oxygen transport efficiencies in precision-cut liver slice-based organ-on-a-chip devices.

Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device (2012)
Journal Article
Kemp, C., Birch, C., Shaw, K. J., Nixon, G. J., Docker, P. T., Greenman, J., Huggett, J. F., Haswell, S. J., Foy, C. A., & Dyer, C. E. (2012). Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device. Analytical Methods, 4(7), 2141-2144. https://doi.org/10.1039/c2ay25075f

Urine is a preferred specimen for nucleic acid-based detection of sexually transmitted infections (STIs) but represents a challenge for microfluidic devices due to low analyte concentrations. We present an extraction methodology enabling rapid on-chi... Read More about Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device.

Reply to “Analysis of NO and its metabolites by mass spectrometry. Comment on ‘Detection of nitric oxide in tissue samples by ESI-MS’” (2011)
Journal Article
Zheng, S., Webster, A., Zheng, S., Welham, K. J., Dyer, C. E., Greenman, J., Haswell, S. J., Webster, A., Zheng, S., Welham, K. J., Dyer, C. E., Greenman, J., Haswell, S. J., Zheng, S., Webster, A., Welham, K. J., Dyer, C. E., Greenman, J., Haswell, S. J., Zheng, S., …Haswell, S. J. (2011). Reply to “Analysis of NO and its metabolites by mass spectrometry. Comment on ‘Detection of nitric oxide in tissue samples by ESI-MS’”. Analyst, 136(2), 411-411. https://doi.org/10.1039/c0an00729c

Comment by Tsikas et al. (Analyst, 2011, DOI: 10.1039/c0an00411a) on the preliminary work by Shen and colleagues (Analyst, 2010, 135, 302) describing the use of ESI-MS/MS for the detection of methylpiperazinobenzenediamine, as a probe for NO in tissu... Read More about Reply to “Analysis of NO and its metabolites by mass spectrometry. Comment on ‘Detection of nitric oxide in tissue samples by ESI-MS’”.

On-chip integrated labelling, transport and detection of tumour cells (2011)
Journal Article
Woods, J., Docker, P. T., Dyer, C. E., Haswell, S. J., & Greenman, J. (2011). On-chip integrated labelling, transport and detection of tumour cells. ELECTROPHORESIS, 32(22), 3188-3195. https://doi.org/10.1002/elps.201100172

Microflow cytometry represents a promising tool for the investigation of diagnostic and prognostic cellular cancer markers, particularly if integrated within a device that allows primary cells to be freshly isolated from the solid tumour biopsies tha... Read More about On-chip integrated labelling, transport and detection of tumour cells.

Development of a real-world direct interface for integrated DNA extraction and amplification in a microfluidic device (2010)
Journal Article
Shaw, K. J., Joyce, D., Docker, P. T., Dyer, C. E., Greenway, G. M., Greenman, J., & Haswell, S. J. (2011). Development of a real-world direct interface for integrated DNA extraction and amplification in a microfluidic device. Lab on a chip, 11(3), 443-448. https://doi.org/10.1039/c0lc00346h

Integrated DNA extraction and amplification have been carried out in a microfluidic device using electro-osmotic pumping (EOP) for fluidic control. All the necessary reagents for performing both DNA extraction and polymerase chain reaction (PCR) ampl... Read More about Development of a real-world direct interface for integrated DNA extraction and amplification in a microfluidic device.

Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling (2010)
Journal Article
Shaw, K. J., Docker, P. T., Yelland, J. V., Dyer, C. E., Greenman, J., Greenway, G. M., & Haswell, S. J. (2010). Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling. Lab on a chip, 10(13), 1725-1728. https://doi.org/10.1039/c000357n

A microwave heating system is described for performing polymerase chain reaction (PCR) in a microfluidic device. The heating system, in combination with air impingement cooling, provided rapid thermal cycling with heating and cooling rates of up to 6... Read More about Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling.

Detection of nitric oxide in tissue samples by ESI-MS (2009)
Journal Article
Shen, Z., Webster, A., Welham, K. J., Dyer, C. E., Greenman, J., & Haswell, S. J. (2010). Detection of nitric oxide in tissue samples by ESI-MS. Analyst, 135(2), 302-305. https://doi.org/10.1039/b917643h

A novel method to determine nitric oxide (NO) in biological tissue samples with minimal interference from the cellular detritus is described. Methylpiperazinobenzenediamine, consisting of an o-phenylenediamine and a methyl piperazine group, was chose... Read More about Detection of nitric oxide in tissue samples by ESI-MS.

Development of a gel-to-gel electro-kinetic pinched injection method for an integrated micro-fluidic based DNA analyser (2009)
Journal Article
Oakley, J. A., Robinson, S., Dyer, C. E., Greenman, J., Greenway, G. M., & Haswell, S. J. (2009). Development of a gel-to-gel electro-kinetic pinched injection method for an integrated micro-fluidic based DNA analyser. Analytica Chimica Acta, 652(1-2), 239-244. https://doi.org/10.1016/j.aca.2009.07.063

An integrated gel supported micro-fluidic system is reported, in which PCR products can be efficiently injected into a capillary electrophoresis device. The gel supported system is designed to provide greater stability to reagents during long periods... Read More about Development of a gel-to-gel electro-kinetic pinched injection method for an integrated micro-fluidic based DNA analyser.

The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths (2009)
Journal Article
Shaw, K. J., Thain, L., Dyer, C., Docker, P. T., Greenman, J., Greenway, G. M., & Haswell, S. J. (2009). The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths. Analytica Chimica Acta, 652(1-2), 231-233. https://doi.org/10.1016/j.aca.2009.03.038

DNA extraction was carried out on silica-based monoliths within a microfluidic device. Solid-phase DNA extraction methodology was applied in which the DNA binds to silica in the presence of a chaotropic salt, such as guanidine hydrochloride, and is e... Read More about The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths.

Development of a bi-functional silica monolith for electro-osmotic pumping and DNA clean-up/extraction using gel-supported reagents in a microfluidic device (2009)
Journal Article
Oakley, J. A., Shaw, K. J., Docker, P. T., Dyer, C. E., Greenman, J., Greenway, G. M., & Haswell, S. J. (2009). Development of a bi-functional silica monolith for electro-osmotic pumping and DNA clean-up/extraction using gel-supported reagents in a microfluidic device. Lab on a chip, 9(11), 1596-1600. https://doi.org/10.1039/b820553a

A silica monolith used to support both electro-osmotic pumping (EOP) and the extraction/elution of DNA coupled with gel-supported reagents is described. The benefits of the combined EOP extraction/elution system were illustrated by combining DNA extr... Read More about Development of a bi-functional silica monolith for electro-osmotic pumping and DNA clean-up/extraction using gel-supported reagents in a microfluidic device.

Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device (2009)
Journal Article
Shaw, K. J., Docker, P. T., Haswell, S. J., Joyce, D. A., Dyer, C. E., Greenman, J., & Greenway, G. M. (2009). Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device. Lab on a chip, 9(23), 3430-3432. https://doi.org/10.1039/b913309g

A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was... Read More about Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device.