All Outputs (61)

Gendered farmer perceptions towards soil nutrition and willingness to pay for a cafetière-style filter system for in-situ soil testing: evidence from Central Kenya (2024)
Journal Article
Kamau, P., Ndirangu, I., Richardson, S., Pamme, N., & Gitaka, J. (2024). Gendered farmer perceptions towards soil nutrition and willingness to pay for a cafetière-style filter system for in-situ soil testing: evidence from Central Kenya. Heliyon, 10(18), Article e37568. https://doi.org/10.1016/j.heliyon.2024.e37568

Soil nutrition is a key pillar in agricultural productivity. However, point-of-need testing for soil nutrition is not readily available in resource-limited settings such as Kenya. We set out to study the perceived need for soil testing among farmers... Read More about Gendered farmer perceptions towards soil nutrition and willingness to pay for a cafetière-style filter system for in-situ soil testing: evidence from Central Kenya.

Citizen-led sampling to monitor phosphate levels in freshwater environments using a simple paper microfluidic device (2021)
Journal Article
Richardson, S., Iles, A., Rotchell, J. M., Charlson, T., Hanson, A., Lorch, M., & Pamme, N. (2021). Citizen-led sampling to monitor phosphate levels in freshwater environments using a simple paper microfluidic device. PLoS ONE, 16(12), Article e0260102. https://doi.org/10.1371/journal.pone.0260102

Contamination of waterways is of increasing concern, with recent studies demonstrating elevated levels of antibiotics, antidepressants, household, agricultural and industrial chemicals in freshwater systems. Thus, there is a growing demand for method... Read More about Citizen-led sampling to monitor phosphate levels in freshwater environments using a simple paper microfluidic device.

Spheroid-on-chip microfluidic technology for the evaluation of the impact of continuous flow on metastatic potential in cancer models in vitro (2021)
Journal Article
Collins, T., Pyne, E., Christensen, M., Iles, A., Pamme, N., & Pires, I. M. (2021). Spheroid-on-chip microfluidic technology for the evaluation of the impact of continuous flow on metastatic potential in cancer models in vitro. Biomicrofluidics, 15(4), Article 044103. https://doi.org/10.1063/5.0061373

The majority of cancer deaths are linked to tumor spread, or metastasis, but 3D in vitro metastasis models relevant to the tumor microenvironment (including interstitial fluid flow) remain an area of unmet need. Microfluidics allows us to introduce c... Read More about Spheroid-on-chip microfluidic technology for the evaluation of the impact of continuous flow on metastatic potential in cancer models in vitro.

Host-pathogen adhesion as the basis of innovative diagnostics for emerging pathogens (2021)
Journal Article
van Belkum, A., Almeida, C., Bardiaux, B., Barrass, S. V., Butcher, S. J., Çaykara, T., …Linke, D. (2021). Host-pathogen adhesion as the basis of innovative diagnostics for emerging pathogens. Diagnostics, 11(7), Article 1259. https://doi.org/10.3390/diagnostics11071259

Infectious diseases are an existential health threat, potentiated by emerging and re-emerging viruses and increasing bacterial antibiotic resistance. Targeted treatment of infectious diseases requires precision diagnostics, especially in cases where... Read More about Host-pathogen adhesion as the basis of innovative diagnostics for emerging pathogens.

Investigating oxygen transport efficiencies in precision-cut liver slice-based organ-on-a-chip devices (2021)
Journal Article
Christensen, M. G., Cawthorne, C., Dyer, C. E., Greenman, J., & Pamme, N. (2021). Investigating oxygen transport efficiencies in precision-cut liver slice-based organ-on-a-chip devices. Microfluidics and Nanofluidics, 25(4), Article 35. https://doi.org/10.1007/s10404-021-02434-x

Microfluidic ‘organ-on-a-chip’ devices hold great potential for better mimicking the continuous flow microenvironment experienced by tissue and cells in vivo, thereby ensuring realistic transport of nutrients and elimination of waste products. Howeve... Read More about Investigating oxygen transport efficiencies in precision-cut liver slice-based organ-on-a-chip devices.

Detection of doxycycline hyclate and oxymetazoline hydrochloride in pharmaceutical preparations via spectrophotometry and microfluidic paper-based analytical device (μPADs) (2020)
Journal Article
Abdulsattar, J. O., Hadi, H., Richardson, S., Iles, A., & Pamme, N. (2020). Detection of doxycycline hyclate and oxymetazoline hydrochloride in pharmaceutical preparations via spectrophotometry and microfluidic paper-based analytical device (μPADs). Analytica Chimica Acta, 1136, 196-204. https://doi.org/10.1016/j.aca.2020.09.045

There is growing demand for simple to operate, sensitive, on-site quantitative assays to investigate concentrations of drug molecules in pharmaceutical preparations for quality assurance. Here, we report on the development of two colorimetric analysi... Read More about Detection of doxycycline hyclate and oxymetazoline hydrochloride in pharmaceutical preparations via spectrophotometry and microfluidic paper-based analytical device (μPADs).

Paper-based analytical devices for colorimetric detection of: S. aureus and E. coli and their antibiotic resistant strains in milk (2020)
Journal Article
Khan, Q. M., Asif, M., Awan, F. R., Khan, Q. . M., Ngamsom, B., & Pamme, N. (2020). Paper-based analytical devices for colorimetric detection of: S. aureus and E. coli and their antibiotic resistant strains in milk. Analyst, 145(22), 7320-7329. https://doi.org/10.1039/d0an01075h

Animal derived milk which is an important part of human diet due to its high nutritional value not only supports humans but also presents a growth environment for pathogenic bacteria. Milk may become contaminated with bacteria through udder infection... Read More about Paper-based analytical devices for colorimetric detection of: S. aureus and E. coli and their antibiotic resistant strains in milk.

Microfluidic-Based Electrochemical Immunosensing of Ferritin (2020)
Journal Article
Garg, M., Christensen, M. G., Iles, A., Sharma, A. L., Singh, S., & Pamme, N. (2020). Microfluidic-Based Electrochemical Immunosensing of Ferritin. Biosensors, 10(8), Article 91. https://doi.org/10.3390/bios10080091

Ferritin is a clinically important biomarker which reflects the state of iron in the body and is directly involved with anemia. Current methods available for ferritin estimation are generally not portable or they do not provide a fast response. To co... Read More about Microfluidic-Based Electrochemical Immunosensing of Ferritin.

On-chip electrochemical detection of glucose towards the miniaturised quality control of carbohydrate-based radiotracers (2020)
Journal Article
Patinglag, L., Esfahani, M. M. N., Ragunathan, K., He, P., Brown, N. J., Archibald, S. J., Pamme, N., & Tarn, M. D. (2020). On-chip electrochemical detection of glucose towards the miniaturised quality control of carbohydrate-based radiotracers. Analyst, 145(14), 4920-4930. https://doi.org/10.1039/c9an01881f

The miniaturisation of positron emission tomography (PET) radiotracer production is facilitating a move towards a dose-on-demand strategy that would enable a stratified approach to patient diagnostics, but while the on-chip synthesis steps have been... Read More about On-chip electrochemical detection of glucose towards the miniaturised quality control of carbohydrate-based radiotracers.

Realisation of a sub-wavelength dimple using a 193 nm wavelength photonic nano jet (2020)
Journal Article
Al-Jarwany, Q. A., Mohammed, A. F., Hamza, A. O., Bouillard, J. S., Adawi, A. M., Pamme, N., & Walton, C. D. (2020). Realisation of a sub-wavelength dimple using a 193 nm wavelength photonic nano jet. Chemical Physics Letters, 750, Article 137400. https://doi.org/10.1016/j.cplett.2020.137400

There are many areas of research that benefit from tight focussing of light. We report experimental and computational results of a laser irradiated silica microsphere, 1 μm diameter localised on an SU-8 substrate. A single pulse from an ArF excimer l... Read More about Realisation of a sub-wavelength dimple using a 193 nm wavelength photonic nano jet.

Biosensor for determining average iron content of ferritin by measuring its optical dispersion (2020)
Presentation / Conference Contribution
Gupta, R., Alamrani, N. A., Greenway, G. M., Pamme, N., & Goddard, N. J. Biosensor for determining average iron content of ferritin by measuring its optical dispersion. Presented at SPIE BIOS: Microfluidics, BioMEMS, and Medical Microsystems XVIII, San Francisco, California

© 2020 SPIE. Average iron content of ferritin has a potential to serve as a biomarker for early identification of high-risk trauma patients at point-of-care (PoC). Appropriate therapies can then be administered to reduce morbidity and mortality. Curr... Read More about Biosensor for determining average iron content of ferritin by measuring its optical dispersion.

FISH and chips: a review of microfluidic platforms for FISH analysis (2020)
Journal Article
Rodriguez-Mateos, P., Azevedo, N. F., Almeida, C., & Pamme, N. (2020). FISH and chips: a review of microfluidic platforms for FISH analysis. Medical Microbiology and Immunology, 209(3), 373-391. https://doi.org/10.1007/s00430-019-00654-1

Fluorescence in situ hybridization (FISH) allows visualization of specific nucleic acid sequences within an intact cell or a tissue section. It is based on molecular recognition between a fluorescently labeled probe that penetrates the cell membrane... Read More about FISH and chips: a review of microfluidic platforms for FISH analysis.

Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya (2019)
Journal Article
Ngamsom, B., Wandera, E. A., Iles, A., Kimani, R., Muregi, F., Gitaka, J., & Pamme, N. (2019). Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya. Analyst, 144(23), 6889-6897. https://doi.org/10.1039/c9an01808e

© The Royal Society of Chemistry 2019. We report the rapid detection (20 min) of Streptococcus agalactiae, Group B Streptococcus (GBS) employing on-chip magnetic isolation of GBS based on immiscible filtration assisted by surface tension (IFAST), fol... Read More about Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya.

A feasibility study of a leaky waveguide aptasensor for thrombin (2019)
Journal Article
Alamrani, N. A., Greenway, G. M., Pamme, N., Goddard, N. J., & Gupta, R. (2019). A feasibility study of a leaky waveguide aptasensor for thrombin. Analyst, 144(20), 6048-6054. https://doi.org/10.1039/c9an01421g

This proof-of-principle study demonstrates the feasibility of a leaky waveguide (LW) aptasensor, where aptamers were immobilised in a mesoporous chitosan waveguiding film for the detection of thrombin. This work has demonstrated that aptamers immobil... Read More about A feasibility study of a leaky waveguide aptasensor for thrombin.

“Learning on a chip:” Microfluidics for formal and informal science education (2019)
Journal Article
Rackus, D. G., Riedel-Kruse, I. H., & Pamme, N. (2019). “Learning on a chip:” Microfluidics for formal and informal science education. Biomicrofluidics, 13(4), Article 041501. https://doi.org/10.1063/1.5096030

© 2019 Author(s). Microfluidics is a technique for the handling of small volumes of liquids on the order of picoliters to nanoliters and has impact for miniaturized biomedical science and fundamental research. Because of its multi- and interdisciplin... Read More about “Learning on a chip:” Microfluidics for formal and informal science education.

A method for determining average iron content of ferritin by measuring its optical dispersion (2019)
Journal Article
Gupta, R., Alamrani, N. A., Greenway, G. M., Pamme, N., & Goddard, N. J. (2019). A method for determining average iron content of ferritin by measuring its optical dispersion. Analytical chemistry, 91(11), 7366-7372. https://doi.org/10.1021/acs.analchem.9b01231

© 2019 American Chemical Society. We report a method where the refractive index increments of an iron storage protein, ferritin, and apoferritin (ferritin minus iron) were measured over the wavelength range of 450-678 nm to determine the average iron... Read More about A method for determining average iron content of ferritin by measuring its optical dispersion.

Two-step numerical approach to predict ferrofluid droplet generation and manipulation inside multilaminar flow chambers (2019)
Journal Article
Gómez-Pastora, J., Amiri Roodan, V., Karampelas, I. H., Alorabi, A. Q., Tarn, M. D., Iles, A., Bringas, E., Paunov, V. N., Pamme, N., Furlani, E. P., & Ortiz, I. (2019). Two-step numerical approach to predict ferrofluid droplet generation and manipulation inside multilaminar flow chambers. Journal of physical chemistry. C, 123(15), 10065-10080. https://doi.org/10.1021/acs.jpcc.9b01393

Copyright © 2019 American Chemical Society. In this study, a computational fluid dynamics approach is implemented to investigate the dynamic behavior of continuous-flow droplet microfluidics. The developed approach predicts both droplet generation an... Read More about Two-step numerical approach to predict ferrofluid droplet generation and manipulation inside multilaminar flow chambers.

Stereolithographic 3D printing of extrinsically self-healing composites (2019)
Journal Article
Sanders, P., Young, A. J., Qin, Y., Fancey, K. S., Reithofer, M. R., Guillet-Nicolas, R., Kleitz, F., Pamme, N., & Chin, J. M. (2019). Stereolithographic 3D printing of extrinsically self-healing composites. Scientific reports, 9(1), Article 388. https://doi.org/10.1038/s41598-018-36828-9

We demonstrate for the first time the direct stereolithographic 3D printing of an extrinsically self-healing composite, comprised of commercial photocurable resin modified with anisole and PMMA-filled microcapsules. The composites demonstrate solvent... Read More about Stereolithographic 3D printing of extrinsically self-healing composites.

A label-free aptamer-based nanogap capacitive biosensor with greatly diminished electrode polarization effects (2019)
Journal Article
Ghobaei Namhil, Z., Kemp, C., Verrelli, E., Iles, A., Pamme, N., Adawi, A. M., & Kemp, N. (2019). A label-free aptamer-based nanogap capacitive biosensor with greatly diminished electrode polarization effects. Physical chemistry chemical physics : PCCP, 21(2), 681-691. https://doi.org/10.1039/c8cp05510f

A significant impediment to the use of impedance spectroscopy in bio-sensing is the electrode polarization effect that arises from the movement of free ions to the electrode-solution interface, forming an electrical double layer (EDL). The EDL screen... Read More about A label-free aptamer-based nanogap capacitive biosensor with greatly diminished electrode polarization effects.

Microcapsules as assay compartments formed through layer-by-layer deposition (2018)
Journal Article
Alorabi, A. Q., Tarn, M. D., Thomas, M., Paunov, V. N., & Pamme, N. (2018). Microcapsules as assay compartments formed through layer-by-layer deposition. Analytical Methods, 10(44), 5335-5340. https://doi.org/10.1039/c8ay02012d

We investigate the potential of using microcapsules, generated through layer-by-layer assembly, as reaction compartments for bioassays. A streptavidin-biotin and an esterase-fluorescein diacetate (FDA) assay were employed for proof-of-concept. Strept... Read More about Microcapsules as assay compartments formed through layer-by-layer deposition.