All Outputs (104)

Thalidomide enhances cyclophosphamide and dexamethasone-mediated cytotoxicity towards cultured chronic lymphocytic leukaemia cells (2010)
Journal Article
Pointon, J. C., Eagle, G., Bailey, J., Evans, P., Allsup, D., & Greenman, J. (2010). Thalidomide enhances cyclophosphamide and dexamethasone-mediated cytotoxicity towards cultured chronic lymphocytic leukaemia cells. Oncology Reports, 24(5), 1315-1321. https://doi.org/10.3892/or_00000988

Numerous chemotherapeutic regimens exist for the treatment of symptomatic or progressive chronic lymphocytic leukaemia (CLL). However, once the disease becomes refractory to nucleoside-based therapy the prognosis is poor. In this study we investigate... Read More about Thalidomide enhances cyclophosphamide and dexamethasone-mediated cytotoxicity towards cultured chronic lymphocytic leukaemia cells.

Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action (2010)
Journal Article
Smith, K., Malatesti, N., Cauchon, N., Hunting, D., Lecomte, R., van Lier, J. E., Greenman, J., & Boyle, R. W. (2011). Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action. Immunology, 132(2), 256-265. https://doi.org/10.1111/j.1365-2567.2010.03359.x

Two cationic porphyrins bearing an isothiocyanate group for conjugation to monocolonal antibodies have been synthesized. The two porphyrins conjugated efficiently to three monoclonal antibodies (anti-CD104, anti-CD146 and anti-CD326), which recognize... Read More about Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.

Microfluidic perfusion system for maintaining viable heart tissue with real-time electrochemical monitoring of reactive oxygen species (2010)
Journal Article
Cheah, L.-T., Dou, Y. H., Seymour, A. M. L., Dyer, C. E., Haswell, S. J., Wadhawan, J. D., & Greenman, J. (2010). Microfluidic perfusion system for maintaining viable heart tissue with real-time electrochemical monitoring of reactive oxygen species. Lab on a chip, 10(20), 2720-2726. https://doi.org/10.1039/c004910g

A microfluidic device has been developed to maintain viable heart tissue samples in a biomimetic microenvironment. This device allows rat or human heart tissue to be studied under pseudo in vivo conditions. Effluent levels of lactate dehydrogenase an... Read More about Microfluidic perfusion system for maintaining viable heart tissue with real-time electrochemical monitoring of reactive oxygen species.

Development of microfluidic devices for biomedical and clinical application (2010)
Journal Article
Webster, A., Greenman, J., & Haswell, S. J. (2011). Development of microfluidic devices for biomedical and clinical application. Journal of Chemical Technology and Biotechnology, 86(1), 10-17. https://doi.org/10.1002/jctb.2482

This review focuses on the development and use of microfluidic devices within a clinical setting. The underlying theoretical background of microfluidics is briefly elucidated. The materials and techniques used to fabricate the devices and their appli... Read More about Development of microfluidic devices for biomedical and clinical application.

A microfluidic device for tissue biopsy culture and interrogation (2010)
Journal Article
Webster, A., Dyer, C. E., Haswell, S. J., & Greenman, J. (2010). A microfluidic device for tissue biopsy culture and interrogation. Analytical Methods, 2(8), 1005-1007. https://doi.org/10.1039/c0ay00293c

This communication reports the development of a microfluidic device capable of maintaining the long-term culture of viable tissue biopsies. Tissue-based models will enable evaluation of cell-cell and cell-matrix interactions within multi-cellular sys... Read More about A microfluidic device for tissue biopsy culture and interrogation.

Weight-adjusted dalteparin for prevention of vascular thromboembolism in advanced pancreatic cancer patients decreases serum tissue factor and serum-mediated induction of cancer cell invasion (2010)
Journal Article
Maraveyas, A., Ettelaie, C., Echrish, H., Li, C., Gardiner, E., Greenman, J., & Madden, L. A. (2010). Weight-adjusted dalteparin for prevention of vascular thromboembolism in advanced pancreatic cancer patients decreases serum tissue factor and serum-mediated induction of cancer cell invasion. Blood Coagulation and Fibrinolysis, 21(5), 452-458. https://doi.org/10.1097/mbc.0b013e328338dc49

The aim of the present study was to assess the role of tissue factor and serum-induced cell invasion in patients with advanced pancreatic cancer (APC). A cohort of 39 patients with APC, without thrombosis, receiving chemotherapy, were entered in a ra... Read More about Weight-adjusted dalteparin for prevention of vascular thromboembolism in advanced pancreatic cancer patients decreases serum tissue factor and serum-mediated induction of cancer cell invasion.

Serum IL10 and circulating CD4+CD25high regulatory T cell numbers as predictors of clinical outcome and survival in patients with head and neck squamous cell carcinoma (2010)
Journal Article
Alhamarneh, O., Agada, F., Madden, L., Stafford, N., & Greenman, J. (2011). Serum IL10 and circulating CD4+CD25high regulatory T cell numbers as predictors of clinical outcome and survival in patients with head and neck squamous cell carcinoma. Head & neck, 33(3), 415-423. https://doi.org/10.1002/hed.21464

Background. Patients with head and neck squamous cell carcinoma (HNSCC) commonly have an imbalance in T helper (Th)1/Th2-type cytokines and elevated levels of CD4 + CD25 high regulatory T cells (Treg). Here, we investigated the association of circul... Read More about Serum IL10 and circulating CD4+CD25high regulatory T cell numbers as predictors of clinical outcome and survival in patients with head and neck squamous cell carcinoma.

Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling (2010)
Journal Article
Shaw, K. J., Docker, P. T., Yelland, J. V., Dyer, C. E., Greenman, J., Greenway, G. M., & Haswell, S. J. (2010). Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling. Lab on a chip, 10(13), 1725-1728. https://doi.org/10.1039/c000357n

A microwave heating system is described for performing polymerase chain reaction (PCR) in a microfluidic device. The heating system, in combination with air impingement cooling, provided rapid thermal cycling with heating and cooling rates of up to 6... Read More about Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling.

Alterations in the Th1/Th2 balance in breast cancer patients using reflexology and scalp massage (2010)
Journal Article
Green, V. L., Alexandropoulou, A., Walker, M. B., Walker, A. A., Sharp, D. M., Walker, L. G., & Greenman, J. (2010). Alterations in the Th1/Th2 balance in breast cancer patients using reflexology and scalp massage. Experimental and Therapeutic Medicine, 1(1), 97-108. https://doi.org/10.3892/etm_00000018

The diagnosis and treatment of breast cancer can adversely affect quality of life Here the aim was to determine the effects of reflexology on host defences and endocrine function in women with early breast cancer Six weeks after surgery for early bre... Read More about Alterations in the Th1/Th2 balance in breast cancer patients using reflexology and scalp massage.

Detection of nitric oxide in tissue samples by ESI-MS (2009)
Journal Article
Shen, Z., Webster, A., Welham, K. J., Dyer, C. E., Greenman, J., & Haswell, S. J. (2010). Detection of nitric oxide in tissue samples by ESI-MS. Analyst, 135(2), 302-305. https://doi.org/10.1039/b917643h

A novel method to determine nitric oxide (NO) in biological tissue samples with minimal interference from the cellular detritus is described. Methylpiperazinobenzenediamine, consisting of an o-phenylenediamine and a methyl piperazine group, was chose... Read More about Detection of nitric oxide in tissue samples by ESI-MS.

Immobilized anthraquinone for redox mediation of horseradish peroxidase for hydrogen peroxide sensing (2009)
Journal Article
Dou, Y., Haswell, S., Greenman, J., & Wadhawan, J. (2009). Immobilized anthraquinone for redox mediation of horseradish peroxidase for hydrogen peroxide sensing. Electrochemistry communications, 11(10), 1976-1981. https://doi.org/10.1016/j.elecom.2009.08.032

The detection of hydrogen peroxide is detailed using horseradish peroxidase and anthraquinone. Both species are immobilized on a glassy carbon electrode substrate. This dual immobilization gives rise to lower detection limits compared with the situat... Read More about Immobilized anthraquinone for redox mediation of horseradish peroxidase for hydrogen peroxide sensing.

Development of a gel-to-gel electro-kinetic pinched injection method for an integrated micro-fluidic based DNA analyser (2009)
Journal Article
Oakley, J. A., Robinson, S., Dyer, C. E., Greenman, J., Greenway, G. M., & Haswell, S. J. (2009). Development of a gel-to-gel electro-kinetic pinched injection method for an integrated micro-fluidic based DNA analyser. Analytica Chimica Acta, 652(1-2), 239-244. https://doi.org/10.1016/j.aca.2009.07.063

An integrated gel supported micro-fluidic system is reported, in which PCR products can be efficiently injected into a capillary electrophoresis device. The gel supported system is designed to provide greater stability to reagents during long periods... Read More about Development of a gel-to-gel electro-kinetic pinched injection method for an integrated micro-fluidic based DNA analyser.

COX-2 specific inhibitors enhance the cytotoxic effects of pemetrexed in mesothelioma cell lines (2009)
Journal Article
O'Kane, S. L., Eagle, G. L., Greenman, J., Lind, M. J., & Cawkwell, L. (2010). COX-2 specific inhibitors enhance the cytotoxic effects of pemetrexed in mesothelioma cell lines. Lung Cancer, 67(2), 160-165. https://doi.org/10.1016/j.lungcan.2009.04.008

BACKGROUND Although malignant pleural mesothelioma is a rare tumour, its incidence is increasing. The prognosis remains very poor with an average survival of 10 months from diagnosis. The choice of chemotherapy regimens for mesothelioma patients is l... Read More about COX-2 specific inhibitors enhance the cytotoxic effects of pemetrexed in mesothelioma cell lines.

CXCR4 antagonists: a new generation of configurationally restricted bis-azamacrocyclic compounds (2009)
Journal Article
Khan, A., Nicholson, G., McRobbie, G., Greenman, J., Pannecouque, C., Daelemans, D., Schols, D., De CLercq, E., Hubin, T. J., & Archibald, S. J. (2009). CXCR4 antagonists: a new generation of configurationally restricted bis-azamacrocyclic compounds. Antiviral research, 82(2), A59-A60. https://doi.org/10.1016/j.antiviral.2009.02.141

AMD3100 is a bis-azamacrocyclic compound that has been demonstrated to be a highly effective antagonist of the CXCR4 chemokine receptor. The two azamacrocyclic rings have been shown to interact with aspartate residues on the receptor via hydrogen bon... Read More about CXCR4 antagonists: a new generation of configurationally restricted bis-azamacrocyclic compounds.

The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths (2009)
Journal Article
Shaw, K. J., Thain, L., Dyer, C., Docker, P. T., Greenman, J., Greenway, G. M., & Haswell, S. J. (2009). The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths. Analytica Chimica Acta, 652(1-2), 231-233. https://doi.org/10.1016/j.aca.2009.03.038

DNA extraction was carried out on silica-based monoliths within a microfluidic device. Solid-phase DNA extraction methodology was applied in which the DNA binds to silica in the presence of a chaotropic salt, such as guanidine hydrochloride, and is e... Read More about The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths.

Development of a bi-functional silica monolith for electro-osmotic pumping and DNA clean-up/extraction using gel-supported reagents in a microfluidic device (2009)
Journal Article
Oakley, J. A., Shaw, K. J., Docker, P. T., Dyer, C. E., Greenman, J., Greenway, G. M., & Haswell, S. J. (2009). Development of a bi-functional silica monolith for electro-osmotic pumping and DNA clean-up/extraction using gel-supported reagents in a microfluidic device. Lab on a chip, 9(11), 1596-1600. https://doi.org/10.1039/b820553a

A silica monolith used to support both electro-osmotic pumping (EOP) and the extraction/elution of DNA coupled with gel-supported reagents is described. The benefits of the combined EOP extraction/elution system were illustrated by combining DNA extr... Read More about Development of a bi-functional silica monolith for electro-osmotic pumping and DNA clean-up/extraction using gel-supported reagents in a microfluidic device.

Binding Optimization through Coordination Chemistry: CXCR4 Chemokine Receptor Antagonists from Ultrarigid Metal Complexes (2009)
Journal Article
Khan, A., Nicholson, G., McRobbie, G., Pannecouque, C., De Clercq, E., Ullom, R., Maples, D. L., Maples, R. D., Silversides, J. D., Hubin, T. J., Archibald, S. J., Madden, L., & Greenman, J. (2009). Binding Optimization through Coordination Chemistry: CXCR4 Chemokine Receptor Antagonists from Ultrarigid Metal Complexes. Journal of the American Chemical Society, 131(10), 3416-3417. https://doi.org/10.1021/ja807921k

A new copper(II) containing bis-macrocyclic CXCR4 chemokine receptor antagonist is shown to have improved binding properties to the receptor protein in comparison to the drug AMD3100 (Plerixafor, Mozobil). The interaction of the metallodrug has been... Read More about Binding Optimization through Coordination Chemistry: CXCR4 Chemokine Receptor Antagonists from Ultrarigid Metal Complexes.

pH testing for detecting the position of nasogastric tubes in adults and children (2009)
Journal Article
Kalia, S., Hall, J., El-Habbal, M., Imrie, C., Arabiat, D. H., Shields, L., Allgar, V., Wadhawan, J., Greenman, J., & Elliott, B. (2009). pH testing for detecting the position of nasogastric tubes in adults and children. Cochrane Database of Systematic Reviews, 28126. https://doi.org/10.1002/14651858.cd007660

This is the protocol for a review and there is no abstract. The objectives are as follows: Our objective is to determine if there is sufficient evidence to recommend the use of pH testing (the intervention under scrutiny) for verification of correct... Read More about pH testing for detecting the position of nasogastric tubes in adults and children.

Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device (2009)
Journal Article
Shaw, K. J., Docker, P. T., Haswell, S. J., Joyce, D. A., Dyer, C. E., Greenman, J., & Greenway, G. M. (2009). Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device. Lab on a chip, 9(23), 3430-3432. https://doi.org/10.1039/b913309g

A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was... Read More about Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device.

Investigation of the mechanisms of tissue factor-mediated evasion of tumour cells from cellular cytotoxicity (2008)
Journal Article
Li, C., Collier, M. E. W., Frentzou, G. A., Greenman, J., & Ettelaie, C. (2008). Investigation of the mechanisms of tissue factor-mediated evasion of tumour cells from cellular cytotoxicity. Cancer Immunology, Immunotherapy, 57(9), 1347-1355. https://doi.org/10.1007/s00262-008-0469-6

Aims We previously reported that overexpression of tissue factor (TF) protected HT29 tumour cells from cellular cytotoxicity through a mechanism requiring the presence of the cytoplasmic domain of TF. In this investigation the mechanism of TF-mediate... Read More about Investigation of the mechanisms of tissue factor-mediated evasion of tumour cells from cellular cytotoxicity.