Rapid and direct magnetization of GFP-reporter yeast for micro-screening systems

García-Alonso, Javier; Fakhrullin, Rawil F.; Paunov, Vesselin N.

doi:10.1016/j.bios.2009.11.016

Adsorption of shape-anisotropic and porous particles at the air–water and the decane–water interface studied by the gel trapping technique (2013)
Journal Article
Sharp, E. L., Al-Shehri, H., Horozov, T., Stoyanov, S. D., & Paunov, V. N. (2014). Adsorption of shape-anisotropic and porous particles at the air–water and the decane–water interface studied by the gel trapping technique. RSC advances, 4(5), 2205-2213. https://doi.org/10.1039/c3ra44563a

We have studied the attachment and orientation of anisotropic and porous microparticles at liquid surfaces by using the gel trapping technique (GTT). This technique involves spreading of the microparticles of interest at the liquid interface, subsequ... Read More about Adsorption of shape-anisotropic and porous particles at the air–water and the decane–water interface studied by the gel trapping technique.

Fabrication of viable cyborg cells with cyclodextrin functionality (2013)
Journal Article
Mathapa, B. G., & Paunov, V. N. (2014). Fabrication of viable cyborg cells with cyclodextrin functionality. Biomaterials science / Royal Society of Chemistry, 2(2), 212-219. https://doi.org/10.1039/c3bm60162e

We describe two alternative methods for surface functionalisation of Saccharomyces cerevisiae cells with cyclodextrin molecules without affecting the cell viability. The first strategy involved using epichlorohydrin as a cross-linking agent which bin... Read More about Fabrication of viable cyborg cells with cyclodextrin functionality.

Cyclodextrin stabilised emulsions and cyclodextrinosomes (2013)
Journal Article
Mathapa, B. G., & Paunov, V. N. (2013). Cyclodextrin stabilised emulsions and cyclodextrinosomes. Physical chemistry chemical physics : PCCP, 15(41), 17903-17914. https://doi.org/10.1039/c3cp52116h

We report the preparation of o/w emulsions stabilised by microcrystals of cyclodextrin-oil inclusion complexes. The inclusion complexes are formed by threading cyclodextrins from the aqueous phase on n-tetradecane or silicone oil molecules from the e... Read More about Cyclodextrin stabilised emulsions and cyclodextrinosomes.

Sound absorption properties of porous composites fabricated by a hydrogel templating technique (2013)
Journal Article
Rutkevicius, M., Mehl, G. H., Paunov, V. N., Qin, Q., Rubini, P. A., Stoyanov, S. D., & Petkov, J. (2013). Sound absorption properties of porous composites fabricated by a hydrogel templating technique. Journal of Materials Research, 28(17), 2409-2414. https://doi.org/10.1557/jmr.2013.194

We have used a hydrogel templating technique followed by the subsequent evaporation of water present to fabricate porous cement and porous PDMS composites, and we have analyzed their sound absorption properties. All experiments were carried out with... Read More about Sound absorption properties of porous composites fabricated by a hydrogel templating technique.

Shape recognition of microbial cells by colloidal cell imprints (2013)
Journal Article
Borovička, J., Stoyanov, S. D., & Paunov, V. N. (2013). Shape recognition of microbial cells by colloidal cell imprints. Nanoscale, 5(18), 8560-8568. https://doi.org/10.1039/c3nr01893h

We have engineered a class of colloids which can recognize the shape and size of targeted microbial cells and selectively bind to their surfaces. These imprinted colloid particles, which we called “colloid antibodies”, were fabricated by partial frag... Read More about Shape recognition of microbial cells by colloidal cell imprints.

Nanoantibiotic particles for shape and size recognition of pathogens (2013)
Journal Article
Borovicka, J., Paunov, V., & Stoyanov, S. D. (2013). Nanoantibiotic particles for shape and size recognition of pathogens. MRS Online Proceedings Library, 1498, 127 - 132. https://doi.org/10.1557/opl.2013.14

We have developed a novel class of colloidal particles capable of shape and size recognition as well as specific binding to the target cells. These colloid particles were fabricated using a nanoimprinting technology which yields inorganic imprints of... Read More about Nanoantibiotic particles for shape and size recognition of pathogens.

Cyborg cells: functionalisation of living cells with polymers and nanomaterials (2012)
Journal Article
Fakhrullin, R. F., Zamaleeva, A. I., Minullina, R. T., Konnova, S. A., & Paunov, V. N. (2012). Cyborg cells: functionalisation of living cells with polymers and nanomaterials. Chemical Society Reviews, 41(11), 4189. https://doi.org/10.1039/c2cs15264a

Living cells interfaced with a range of polyelectrolyte coatings, magnetic and noble metal nanoparticles, hard mineral shells and other complex nanomaterials can perform functions often completely different from their original specialisation. Such “c... Read More about Cyborg cells: functionalisation of living cells with polymers and nanomaterials.

Adsorption of sterically stabilized latex particles at liquid surfaces: Effects of steric stabilizer surface coverage, particle size, and chain length on particle wettability (2012)
Journal Article
Horozov, T. S., Paunov, V. N., Armes, S. P., Borovicka, J., Reed, K. M., Thompson, K. L., & Walsh, A. (2012). Adsorption of sterically stabilized latex particles at liquid surfaces: Effects of steric stabilizer surface coverage, particle size, and chain length on particle wettability. Langmuir : the ACS journal of surfaces and colloids, 28(18), 7291-7298. https://doi.org/10.1021/la300735u

A series of five near-monodisperse sterically stabilized polystyrene (PS) latexes were synthesized using three well-defined poly(glycerol monomethacrylate) (PGMA) macromonomers with mean degrees of polymerization (DP) of 30, 50, or 70. The surface co... Read More about Adsorption of sterically stabilized latex particles at liquid surfaces: Effects of steric stabilizer surface coverage, particle size, and chain length on particle wettability.

Triggered cell release from shellac–cell composite microcapsules (2012)
Journal Article
Hamad, S. A., Stoyanov, S. D., & Paunov, V. N. (2012). Triggered cell release from shellac–cell composite microcapsules. Soft matter, 8(18), 5069-5077. https://doi.org/10.1039/c2sm07488e

We report the fabrication of novel shellac-cell composite microcapsules with programmed release of cells upon change of pH in a narrow range. The microcapsules were prepared from yeast cells as a model for probiotics combined with aqueous solution of... Read More about Triggered cell release from shellac–cell composite microcapsules.

Fabrication of novel lightweight composites by a hydrogel templating technique (2012)
Journal Article
Rutkevicius, M., Munusami, S. K., Watson, Z., Field, A. D., Salt, M., Stoyanov, S. D., …Paunov, V. N. (2012). Fabrication of novel lightweight composites by a hydrogel templating technique. Materials research bulletin, 47(4), 980-986. https://doi.org/10.1016/j.materresbull.2012.01.014

A non-conventional way of preparation of lightweight porous materials by templating hydrogels with a range of hydrophilic and hydrophobic scaffolding materials was explored. Sub-millimetre hydrogel slurries of polyacrylamide and gellan gum were templ... Read More about Fabrication of novel lightweight composites by a hydrogel templating technique.

Encapsulation of living cells into sporopollenin microcapsules (2011)
Journal Article
Hamad, S. A., Dyab, A. F., Stoyanov, S. D., & Paunov, V. N. (2011). Encapsulation of living cells into sporopollenin microcapsules. Journal of Materials Chemistry, 21(44), 18018-18023. https://doi.org/10.1039/c1jm13719k

We demonstrate that living cells can be encapsulated inside sporopollenin microcapsules derived from Lycopodium clavatum. To encapsulate large objects like cells, the sporopollenin particles are compressed into a pellet which forces their trilite sca... Read More about Encapsulation of living cells into sporopollenin microcapsules.

Functional artificial free-standing yeast biofilms (2011)
Journal Article
Konnova, S. A., Kahraman, M., Zamaleeva, A. I., Culha, M., Paunov, V. N., & Fakhrullin, R. F. (2011). Functional artificial free-standing yeast biofilms. Colloids and surfaces. B, Biointerfaces, 88(2), 656-663. https://doi.org/10.1016/j.colsurfb.2011.07.056

Here we report fabrication of artificial free-standing yeast biofilms built using sacrificial calcium carbonate-coated templates and layer-by-layer assembly of extracellular matrix-mimicking polyelectro lyte multilayers. The free-standing biofilms ar... Read More about Functional artificial free-standing yeast biofilms.

Measuring the three-phase contact angle of nanoparticles at fluid interfaces (2010)
Journal Article
Arnaudov, L. N., Cayre, O. J., Cohen Stuart, M. A., Stoyanov, S. D., & Paunov, V. N. (2010). Measuring the three-phase contact angle of nanoparticles at fluid interfaces. Physical chemistry chemical physics : PCCP, 12(2), 328-331. https://doi.org/10.1039/b917353f

We report a generic technique to image and study the wettability of spherical nanoparticles adsorbed at liquid surfaces and demonstrate that nanoparticle monolayers can be imprinted at air-water and oil-water interfaces and their three-phase contact... Read More about Measuring the three-phase contact angle of nanoparticles at fluid interfaces.

Functionalization of whole-cell bacterial reporters with magnetic nanoparticles (2010)
Journal Article
Zhang, D., Fakhrullin, R. F., Özmen, M., Wang, H., Wang, J., Paunov, V. N., …Huang, W. E. (2011). Functionalization of whole-cell bacterial reporters with magnetic nanoparticles. Microbial Biotechnology, 4(1), 89-97. https://doi.org/10.1111/j.1751-7915.2010.00228.x

We developed a biocompatible and highly efficient approach for functionalization of bacterial cell wall with magnetic nanoparticles (MNPs). Three Acinetobacter baylyi ADP1 chromosomally based bioreporters, which were genetically engineered to express... Read More about Functionalization of whole-cell bacterial reporters with magnetic nanoparticles.

Novel anisotropic materials from functionalised colloidal cellulose and cellulose derivatives (2010)
Journal Article
Holt, B. L., Stoyanov, S. D., Pelan, E., & Paunov, V. N. (2010). Novel anisotropic materials from functionalised colloidal cellulose and cellulose derivatives. Journal of Materials Chemistry, 20(45), 10058-10070. https://doi.org/10.1039/c0jm01022g

This feature article describes selected examples of the properties and the methods of preparation of cellulose micro and nano crystallites (whiskers) and derivatives, with aspects related to fabrication of various anisotropic materials. Nanometre siz... Read More about Novel anisotropic materials from functionalised colloidal cellulose and cellulose derivatives.

Live celloidosome structures based on the assembly of individual cells by colloid interactions (2010)
Journal Article
Fakhrullin, R. F., Brandy, M., Cayre, O. J., Velev, O. D., & Paunov, V. N. (2010). Live celloidosome structures based on the assembly of individual cells by colloid interactions. Physical chemistry chemical physics : PCCP, 12(38), 11912-11922. https://doi.org/10.1039/c0cp00131g

A new class of colloid structures, celloidosomes, has been developed which represent hollow microcapsules whose membranes consist of a single monolayer of living cells. Two routes for producing these structures were designed based on templating of: (... Read More about Live celloidosome structures based on the assembly of individual cells by colloid interactions.

Interfacing Living Unicellular Algae Cells with Biocompatible Polyelectrolyte-Stabilised Magnetic Nanoparticles (2010)
Journal Article
Fakhrullin, R. F., Shlykova, L. V., Zamaleeva, A. I., Nurgaliev, D. K., Osin, Y. N., García-Alonso, J., & Paunov, V. N. (2010). Interfacing Living Unicellular Algae Cells with Biocompatible Polyelectrolyte-Stabilised Magnetic Nanoparticles. Macromolecular bioscience, 10(10), 1257-1264. https://doi.org/10.1002/mabi.201000161

Green algae are a promising platform for the development of biosensors and bioelectronic devices. Here we report a reliable single-step technique for the functionalisation of living unicellular green algae Chlorella pyrenoidosa with biocompatible 15n... Read More about Interfacing Living Unicellular Algae Cells with Biocompatible Polyelectrolyte-Stabilised Magnetic Nanoparticles.

Particle stabilised emulsions studied by WETSEM technique (2010)
Journal Article
Dyab, A. K. F., & Paunov, V. N. (2010). Particle stabilised emulsions studied by WETSEM technique. Soft matter, 6(12), 2613-2615. https://doi.org/10.1039/c0sm00032a

We have developed a new simple method to study the interfacial structure of particle stabilised emulsions in situ by wet scanning electron microscopy (WETSEM) technique for the first time. Particle layer(s), particle location, aggregation and bridgin... Read More about Particle stabilised emulsions studied by WETSEM technique.

Directed assembly of yeast cells into living yeastosomes by microbubble templating (2010)
Journal Article
Brandy, M., Cayre, O. J., Fakhrullin, R. F., Velev, O. D., & Paunov, V. N. (2010). Directed assembly of yeast cells into living yeastosomes by microbubble templating. Soft matter, 6(15), 3494-3498. https://doi.org/10.1039/c0sm00003e

We report the fabrication of yeastosomes—novel multicellular assemblies, which consist of a spherical monolayer of living yeast cells held together by colloid interactions. Our method is based on templating of microbubbles with cells coated with cati... Read More about Directed assembly of yeast cells into living yeastosomes by microbubble templating.

Rapid and direct magnetization of GFP-reporter yeast for micro-screening systems (2009)
Journal Article
García-Alonso, J., Fakhrullin, R. F., & Paunov, V. N. (2010). Rapid and direct magnetization of GFP-reporter yeast for micro-screening systems. Biosensors & bioelectronics, 25(7), 1816-1819. https://doi.org/10.1016/j.bios.2009.11.016

Saccharomyces cerevisiae containing fluorescent markers are ideal candidates for applications in microfluidic screening systems as fluorescence signal is emitted without the need of additional reagents. Here we develop a method for magnetic functiona... Read More about Rapid and direct magnetization of GFP-reporter yeast for micro-screening systems.

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