Outputs (57)

Advances in processes for PET radiotracer synthesis: Separation of [18F]fluoride from enriched [18O]water (2014)
Journal Article
He, P., Haswell, S. J., Pamme, N., & Archibald, S. J. (2014). Advances in processes for PET radiotracer synthesis: Separation of [18F]fluoride from enriched [18O]water. Applied radiation and isotopes : including data, instrumentation, and methods for use in agriculture, industry, and medicine, 91, 64-70. https://doi.org/10.1016/j.apradiso.2014.04.021

Positron emission tomography (PET) is a powerful scientific and clinical tool for the study and visualization of human physiology that can provide important information about metabolism and diseases such as cancer. At present, [ 18 F]fluorodeoxygluco... Read More about Advances in processes for PET radiotracer synthesis: Separation of [18F]fluoride from enriched [18O]water.

Phaseguide assisted liquid lamination for magnetic particle-based assays (2014)
Journal Article
Phurimsak, C., Yildirim, E., Tarn, M. D., Trietsch, S. J., Hankemeier, T., Pamme, N., & Vulto, P. (2014). Phaseguide assisted liquid lamination for magnetic particle-based assays. Lab on a chip, 14(13), 2334-2343. https://doi.org/10.1039/c4lc00139g

We have developed a magnetic particle-based assay platform in which functionalised magnetic particles are transferred sequentially through laminated volumes of reagents and washing buffers. Lamination of aqueous liquids is achieved via the use of pha... Read More about Phaseguide assisted liquid lamination for magnetic particle-based assays.

On-chip processing of particles and cells via multilaminar flow streams (2013)
Journal Article
Tarn, M. D., Lopez-Martinez, M. J., & Pamme, N. (2014). On-chip processing of particles and cells via multilaminar flow streams. Analytical and Bioanalytical Chemistry, 406(1), 139-161. https://doi.org/10.1007/s00216-013-7363-6

The processing of particles, cells, and droplets for reactions, analyses, labeling, and coating is an important aspect of many microfluidic workflows. However, performing multi-step processes is typically a laborious and timeconsuming endeavor. By ex... Read More about On-chip processing of particles and cells via multilaminar flow streams.

The preparation of magnetic iron oxide nanoparticles in microreactors (2013)
Journal Article
Simmons, M., Wiles, C., Rocher, V., Francesconi, M. G., & Watts, P. (2013). The preparation of magnetic iron oxide nanoparticles in microreactors. Journal of flow chemistry, 3(1), 7-10. https://doi.org/10.1556/JFC-D-12-00024

The preparation of magnetic iron oxide nanoparticles within microreactors is reported. The proportion of γ-Fe2O3 and Fe 3O4 in the sample was determined, an important parameter for reproducibility in applications. © 2013 Akadémiai Kiadó.

Radiochemistry on chip: towards dose-on-demand synthesis of PET radiopharmaceuticals (2013)
Journal Article
Arima, V., Pascali, G., Lade, O., Kretschmer, H. R., Bernsdorf, I., Hammond, V., Watts, P., De Leonardis, F., Tarn, M. D., Cvetkovic, B. Z., Dittrich, P. S., Vasovic, N., Duane, R., Jaksic, A., Pamme, N., Zacheo, A., Zizzari, A., Marra, L., Perrone, E., Salvadori, P. A., & Rinaldi, R. (2013). Radiochemistry on chip: towards dose-on-demand synthesis of PET radiopharmaceuticals. Lab on a chip, 13(12), 2328-2336. https://doi.org/10.1039/c3lc00055a

We have developed an integrated microfluidic platform for producing 2-[¹⁸F]-fluoro-2-deoxy-D-glucose (¹⁸F-FDG) in continuous flow from a single bolus of radioactive isotope solution, with constant product yields achieved throughout the operation that... Read More about Radiochemistry on chip: towards dose-on-demand synthesis of PET radiopharmaceuticals.

Integrated DNA extraction and amplification using electrokinetic pumping in a microfluidic device (2011)
Journal Article
Parton, J., Birch, C., Kemp, C., Haswell, S. J., Pamme, N., & Shaw, K. J. (2012). Integrated DNA extraction and amplification using electrokinetic pumping in a microfluidic device. Analytical Methods, 4(1), 96-100. https://doi.org/10.1039/c1ay05552f

An integrated system employing anion exchange for the extraction of DNA from biological samples prior to polymerase chain reaction DNA amplification has been developed, based on microfluidic methodology utilising electrokinetic pumping. In this syste... Read More about Integrated DNA extraction and amplification using electrokinetic pumping in a microfluidic device.

On-chip pre-concentration and complexation of [18F]fluoride ions via regenerable anion exchange particles for radiochemical synthesis of Positron Emission Tomography tracers (2011)
Journal Article
De Leonardis, F., Pascali, G., Salvadori, P. A., Watts, P., & Pamme, N. (2011). On-chip pre-concentration and complexation of [18F]fluoride ions via regenerable anion exchange particles for radiochemical synthesis of Positron Emission Tomography tracers. Journal of Chromatography A, 1218(29), 4714-4719. https://doi.org/10.1016/j.chroma.2011.05.062

Microfluidic approaches have demonstrated a relevant impact on radiochemical reactions involving Positron Emission Tomography (PET) nuclides, due to shorter reaction times and smaller precursor quantities. However, little attention has been given to... Read More about On-chip pre-concentration and complexation of [18F]fluoride ions via regenerable anion exchange particles for radiochemical synthesis of Positron Emission Tomography tracers.

Cell sorting by endocytotic capacity in a microfluidic magnetophoresis device (2011)
Journal Article
Robert, D., Pamme, N., Conjeaud, H., Gazeau, F., Iles, A., & Wilhelm, C. (2011). Cell sorting by endocytotic capacity in a microfluidic magnetophoresis device. Lab on a chip, 11(11), 1902-1910. https://doi.org/10.1039/c0lc00656d

Magnetically labelled cells are finding a wealth of applications for in vitro analysis as well as in vivo treatments. Sorting of cells into subpopulations based on their magnetite loading is an important step in such procedures. Here, we study the so... Read More about Cell sorting by endocytotic capacity in a microfluidic magnetophoresis device.

Flow focussing of particles and cells based on their intrinsic properties using a simple diamagnetic repulsion setup (2011)
Journal Article
Rodríguez-Villarreal, A. I., Tarn, M. D., Madden, L. A., Lutz, J. B., Greenman, J., Samitier, J., & Pamme, N. (2011). Flow focussing of particles and cells based on their intrinsic properties using a simple diamagnetic repulsion setup. Lab on a chip, 11(7), 1240-1248. https://doi.org/10.1039/c0lc00464b

The continuous flow focussing and manipulation of particles and cells are important factors in microfluidic applications for performing accurate and reproducible procedures downstream. Many particle focussing methods require complex setups or channel... Read More about Flow focussing of particles and cells based on their intrinsic properties using a simple diamagnetic repulsion setup.

Microscreening toxicity system based on living magnetic yeast and gradient chips (2010)
Journal Article
García-Alonso, J., Fakhrullin, R. F., Paunov, V. N., Shen, Z., Hardege, J. D., Pamme, N., Haswell, S. J., & Greenway, G. M. (2011). Microscreening toxicity system based on living magnetic yeast and gradient chips. Analytical and Bioanalytical Chemistry, 400(4), 1009-1013. https://doi.org/10.1007/s00216-010-4241-3

There is an increasing demand for easy and cost-effective methods to screen the toxicological impact of the growing number of chemical mixtures being generated by industry. Such a screening method has been developed using viable, genetically modified... Read More about Microscreening toxicity system based on living magnetic yeast and gradient chips.

Rapid, multistep on-chip DNA hybridisation in continuous flow on magnetic particles (2010)
Journal Article
Vojtíšek, M., Iles, A., & Pamme, N. (2010). Rapid, multistep on-chip DNA hybridisation in continuous flow on magnetic particles. Biosensors & bioelectronics, 25(9), 2172-2176. https://doi.org/10.1016/j.bios.2010.01.034

DNA hybridisation is an important tool for bioanalytical research and clinical diagnostics; conventional methods, however, require long incubation times and numerous washing steps, rendering the procedure time consuming and labour intensive. In this... Read More about Rapid, multistep on-chip DNA hybridisation in continuous flow on magnetic particles.

The importance of particle type selection and temperature control for on-chip free-flow magnetophoresis (2009)
Journal Article
Tarn, M. D., Peyman, S. A., Robert, D., Iles, A., Wilhelm, C., & Pamme, N. (2009). The importance of particle type selection and temperature control for on-chip free-flow magnetophoresis. Journal of Magnetism and Magnetic Materials, 321(24), 4115-4122. https://doi.org/10.1016/j.jmmm.2009.08.016

Free-flow magnetophoresis is becoming a popular technique for the separation and manipulation of magnetic particles and materials in microsystems. A wide variety of magnetic particles are commercially available that differ greatly in size and in magn... Read More about The importance of particle type selection and temperature control for on-chip free-flow magnetophoresis.

Mobile magnetic particles as solid-supports for rapid surface-based bioanalysis in continuous flow (2009)
Journal Article
Peyman, S. A., Iles, A., & Pamme, N. (2009). Mobile magnetic particles as solid-supports for rapid surface-based bioanalysis in continuous flow. Lab on a chip, 9(21), 3110-3117. https://doi.org/10.1039/b904724g

An extremely versatile microfluidic device is demonstrated in which multi-step (bio) chemical procedures can be performed in continuous flow. The system operates by generating several co-laminar flow streams, which contain reagents for specific (bio)... Read More about Mobile magnetic particles as solid-supports for rapid surface-based bioanalysis in continuous flow.

Diamagnetic repulsion—A versatile tool for label-free particle handling in microfluidic devices (2009)
Journal Article
Peyman, S. A., Kwan, E. Y., Margarson, O., Iles, A., & Pamme, N. (2009). Diamagnetic repulsion—A versatile tool for label-free particle handling in microfluidic devices. Journal of Chromatography A, 1216(52), 9055-9062. https://doi.org/10.1016/j.chroma.2009.06.039

We report the exploration of diamagnetic repulsion forces for the selective manipulation of microparticles inside microfluidic devices. Diamagnetic materials such as polymers are repelled from magnetic fields, an effect greatly enhanced by suspending... Read More about Diamagnetic repulsion—A versatile tool for label-free particle handling in microfluidic devices.

On-chip diamagnetic repulsion in continuous flow (2009)
Journal Article
Tarn, M. D., Hirota, N., Iles, A., & Pamme, N. (2009). On-chip diamagnetic repulsion in continuous flow. Science and Technology of Advanced Materials, 10(1), 014611 - 014611. https://doi.org/10.1088/1468-6996/10/1/014611

We explore the potential of a microfluidic continuous flow particle separation system based on the repulsion of diamagnetic materials from a high magnetic field. Diamagnetic polystyrene particles in paramagnetic manganese (II) chloride solution were... Read More about On-chip diamagnetic repulsion in continuous flow.

Rapid on-chip multi-step (bio)chemical procedures in continuous flow - manoeuvring particles through co-laminar reagent streams (2008)
Journal Article
Peyman, S. A., Iles, A., & Pamme, N. (2008). Rapid on-chip multi-step (bio)chemical procedures in continuous flow - manoeuvring particles through co-laminar reagent streams. Chemical communications : Chem comm / the Royal Society of Chemistry, 1220-1222. https://doi.org/10.1039/b716532c

We introduce a novel and extremely versatile microfluidic platform in which tedious multi-step biochemical processes can be performed in continuous flow within a fraction of the time required for conventional methods.

Simultaneous bioassays in a microfluidic channel on plugs of different magnetic particles (2007)
Journal Article
Bronzeau, S., & Pamme, N. (2008). Simultaneous bioassays in a microfluidic channel on plugs of different magnetic particles. Analytica Chimica Acta, 609(1), 105-112. https://doi.org/10.1016/j.aca.2007.11.035

Magnetic particles coated with specific biomolecules are often used as solid supports for bioassays but conventional test tube based techniques are time consuming and labour intensive. An alternative is to work on magnetic particle plugs immobilised... Read More about Simultaneous bioassays in a microfluidic channel on plugs of different magnetic particles.