All Outputs (87)

Analysis of the potential of cancer cell lines to release tissue factor-containing microvesicles: correlation with tissue factor and PAR2 expression (2016)
Journal Article
Ettelaie, C., Collier, M. E. W., Featherby, S., Benelhaj, N. E., Greenman, J., & Maraveyas, A. (2016). Analysis of the potential of cancer cell lines to release tissue factor-containing microvesicles: correlation with tissue factor and PAR2 expression. Thrombosis journal, 14(2), Article ARTN 2. https://doi.org/10.1186/s12959-016-0075-3

Background Despite the association of cancer-derived circulating tissue factor (TF)-containing microvesicles and hypercoagulable state, correlations with the incidence of thrombosis remain unclear. Methods In this study the upregulation of TF re... Read More about Analysis of the potential of cancer cell lines to release tissue factor-containing microvesicles: correlation with tissue factor and PAR2 expression.

Prognostic value of the neutrophil-to-lymphocyte ratio in patients with laryngeal squamous cell carcinoma (2015)
Journal Article
Wong, B. Y. W., Stafford, N. D., Green, V. L., & Greenman, J. (2016). Prognostic value of the neutrophil-to-lymphocyte ratio in patients with laryngeal squamous cell carcinoma. Head & neck, 38(S1), E1903-E1908. https://doi.org/10.1002/hed.24346

© 2015 Wiley Periodicals, Inc.. Background The neutrophil/lymphocyte ratio (NLR) has been found to be predictive of survival outcome in a range of tumors. The purpose of this study was to investigate the prognostic value of pretreatment (NLR) in pati... Read More about Prognostic value of the neutrophil-to-lymphocyte ratio in patients with laryngeal squamous cell carcinoma.

On-chip determination of C-reactive protein using magnetic particles in continuous flow (2014)
Journal Article
Phurimsak, C., Tarn, M. D., Peyman, S. A., Greenman, J., & Pamme, N. (2014). On-chip determination of C-reactive protein using magnetic particles in continuous flow. Analytical chemistry, 86(21), 10552-10559. https://doi.org/10.1021/ac5023265

We demonstrate the application of a multilaminar flow platform, in which functionalized magnetic particles are deflected through alternating laminar flow streams of reagents and washing solutions via an external magnet, for the rapid detection of the... Read More about On-chip determination of C-reactive protein using magnetic particles in continuous flow.

Frequency over function : raised levels of CD127low/- regulatory T cells in the tumour microenvironment compared with the periphery of head and neck cancer patients (2014)
Journal Article
Drennan, S., Stafford, N. D., Greenman, J., & Green, V. L. (2014). Frequency over function : raised levels of CD127low/- regulatory T cells in the tumour microenvironment compared with the periphery of head and neck cancer patients. Journal of clinical & cellular immunology, 5(4), Article 241. https://doi.org/10.4172/2155-9899.1000241

Objective: Regulatory T cells (Tregs) are known to infiltrate the tumour microenvironment of many cancers, including head and neck malignancies, and are thought to contribute to the host's impaired anti-tumour immune response. However, their immunosu... Read More about Frequency over function : raised levels of CD127low/- regulatory T cells in the tumour microenvironment compared with the periphery of head and neck cancer patients.

Clinical relevance of immune parameters in the tumor microenvironment of head and neck cancers (2014)
Journal Article
Wallis, S. P., Stafford, N. D., & Greenman, J. (2015). Clinical relevance of immune parameters in the tumor microenvironment of head and neck cancers. Head & neck, 37(3), 449-459. https://doi.org/10.1002/hed.23736

© 2014 Wiley Periodicals, Inc. Background. The tumour microenvironment is a highly complex region where multiple interactions occur between host and cancer cells. The host response is defined by the presence and function of different tumour infiltrat... Read More about Clinical relevance of immune parameters in the tumor microenvironment of head and neck cancers.

Imaging COX-2 expression in cancer using PET/SPECT radioligands: current status and future directions (2013)
Journal Article
Pacelli, A., Greenman, J., Cawthorne, C., & Smith, G. (2014). Imaging COX-2 expression in cancer using PET/SPECT radioligands: current status and future directions. Journal of Labelled Compounds and Radiopharmaceuticals, 57(4), 317-322. https://doi.org/10.1002/jlcr.3160

The role of cyclooxygenase (COX)-2 as a driving force in early tumourigenesis and the current interest in the combination of COX-2 inhibitors with standard therapy in clinical trials creates an urgent need to establish clinically relevant diagnostic... Read More about Imaging COX-2 expression in cancer using PET/SPECT radioligands: current status and future directions.

Analysis of radiation-induced cell death in head and neck squamous cell carcinoma and rat liver maintained in microfluidic devices (2013)
Journal Article
Green, V. L., Greenman, J., Carr, S. D., & Stafford, N. D. (2014). Analysis of radiation-induced cell death in head and neck squamous cell carcinoma and rat liver maintained in microfluidic devices. Otolaryngology and head and neck surgery, 150(1), 73-80. https://doi.org/10.1177/0194599813507427

Objective The aim of this study was to investigate how head and neck squamous cell carcinoma (HNSCC) tissue biopsies maintained in a pseudo in vivo environment within a bespoke microfluidic device respond to radiation treatment. Study Design Feasibi... Read More about Analysis of radiation-induced cell death in head and neck squamous cell carcinoma and rat liver maintained in microfluidic devices.

Increased frequency and suppressive activity of CD127low/- regulatory T cells in the peripheral circulation of patients with head and neck squamous cell carcinoma are associated with advanced stage and nodal involvement (2013)
Journal Article
Drennan, S., Stafford, N. D., Greenman, J., & Green, V. (2013). Increased frequency and suppressive activity of CD127low/- regulatory T cells in the peripheral circulation of patients with head and neck squamous cell carcinoma are associated with advanced stage and nodal involvement. Immunology, 140(3), 335-343. https://doi.org/10.1111/imm.12144

The presence of regulatory T (Treg) cells is thought to be an important mechanism by which head and neck squamous cell carcinoma (HNSCC) successfully evades the immune system. Using multicolour flow cytometry, the frequency and functional capacity of... Read More about Increased frequency and suppressive activity of CD127low/- regulatory T cells in the peripheral circulation of patients with head and neck squamous cell carcinoma are associated with advanced stage and nodal involvement.

Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device (2012)
Journal Article
Kemp, C., Birch, C., Shaw, K. J., Nixon, G. J., Docker, P. T., Greenman, J., Huggett, J. F., Haswell, S. J., Foy, C. A., & Dyer, C. E. (2012). Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device. Analytical Methods, 4(7), 2141-2144. https://doi.org/10.1039/c2ay25075f

Urine is a preferred specimen for nucleic acid-based detection of sexually transmitted infections (STIs) but represents a challenge for microfluidic devices due to low analyte concentrations. We present an extraction methodology enabling rapid on-chi... Read More about Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device.

Increased inducible heat shock protein 72 expression associated with PBMC isolated from patients with haematological tumours (2012)
Journal Article
Madden, L. A., Hayman, Y. A., Underwood, C., Vince, R. V., Greenman, J., Allsup, D., & Ali, S. (2012). Increased inducible heat shock protein 72 expression associated with PBMC isolated from patients with haematological tumours. Scandinavian journal of clinical & laboratory investigation, 72(5), 380-386. https://doi.org/10.3109/00365513.2012.681683

Background. Heat shock protein 72 (Hsp72) is a highly inducible stress protein and molecular chaperone. Cancers have been shown to be associated with increased Hsp72 expression within the tumour itself and this may lead to resistance to apoptosis. Me... Read More about Increased inducible heat shock protein 72 expression associated with PBMC isolated from patients with haematological tumours.

Intraluminal thrombus has a selective influence on matrix metalloproteinases and their inhibitors (tissue inhibitors of matrix metalloproteinases) in the wall of abdominal aortic aneurysms (2012)
Journal Article
Abdul Rahman, M., Mazari, F., Khan, J. A., Madden, L., McCollum, P., Shahin, Y., Chetter, I., Greenman, J., Fagan, M., & Smith, G. (2012). Intraluminal thrombus has a selective influence on matrix metalloproteinases and their inhibitors (tissue inhibitors of matrix metalloproteinases) in the wall of abdominal aortic aneurysms. Annals of vascular surgery, 26(3), 322-329. https://doi.org/10.1016/j.avsg.2011.08.015

BACKGROUND The influence of intraluminal thrombus (ILT) on the proteolytic environment within the wall of an abdominal aortic aneurysm (AAA) is unknown. This is the first study to examine the correlation between ILT thickness and the levels of matrix... Read More about Intraluminal thrombus has a selective influence on matrix metalloproteinases and their inhibitors (tissue inhibitors of matrix metalloproteinases) in the wall of abdominal aortic aneurysms.

Evaluation of heart tissue viability under redox-magnetohydrodynamics conditions: Toward fine-tuning flow in biological microfluidics applications (2012)
Journal Article
Cheah, L. T., Fritsch, I., Haswell, S. J., & Greenman, J. (2012). Evaluation of heart tissue viability under redox-magnetohydrodynamics conditions: Toward fine-tuning flow in biological microfluidics applications. Biotechnology and bioengineering, 109(7), 1827-1834. https://doi.org/10.1002/bit.24426

A microfluidic system containing a chamber for heart tissue biopsies, perfused with Krebs–Henseleit buffer containing glucose and antibiotic (KHGB) using peristaltic pumps and continuously stimulated, was used to evaluate tissue viability under redox... Read More about Evaluation of heart tissue viability under redox-magnetohydrodynamics conditions: Toward fine-tuning flow in biological microfluidics applications.

Voltammetric Immunoassay for the Detection of Protein Biomarkers (2012)
Journal Article
Haswell, S. J., Dou, Y.-H., Wadhawan, J., & Greenman, J. (2012). Voltammetric Immunoassay for the Detection of Protein Biomarkers. Electroanalysis, 24(2), 264-272. https://doi.org/10.1002/elan.201100676

A strategy for a fast (ca. 20 min), specific, electrochemical immunoassay for the cardiac biomarker creatine kinase (CK) and the human cytokine interleukin 10 (IL10) has been developed in this paper. The polyaniline modified gold surface formed from... Read More about Voltammetric Immunoassay for the Detection of Protein Biomarkers.

Reply to “Analysis of NO and its metabolites by mass spectrometry. Comment on ‘Detection of nitric oxide in tissue samples by ESI-MS’” (2011)
Journal Article
Zheng, S., Webster, A., Zheng, S., Welham, K. J., Dyer, C. E., Greenman, J., Haswell, S. J., Webster, A., Zheng, S., Welham, K. J., Dyer, C. E., Greenman, J., Haswell, S. J., Zheng, S., Webster, A., Welham, K. J., Dyer, C. E., Greenman, J., Haswell, S. J., Zheng, S., …Haswell, S. J. (2011). Reply to “Analysis of NO and its metabolites by mass spectrometry. Comment on ‘Detection of nitric oxide in tissue samples by ESI-MS’”. Analyst, 136(2), 411-411. https://doi.org/10.1039/c0an00729c

Comment by Tsikas et al. (Analyst, 2011, DOI: 10.1039/c0an00411a) on the preliminary work by Shen and colleagues (Analyst, 2010, 135, 302) describing the use of ESI-MS/MS for the detection of methylpiperazinobenzenediamine, as a probe for NO in tissu... Read More about Reply to “Analysis of NO and its metabolites by mass spectrometry. Comment on ‘Detection of nitric oxide in tissue samples by ESI-MS’”.

Effect of treatment on systemic cytokines in head and neck squamous cell carcinoma patients (2011)
Journal Article
Green, V. L., Michno, A., Greenman, J., & Stafford, N. D. (2012). Effect of treatment on systemic cytokines in head and neck squamous cell carcinoma patients. Results in immunology, 2, 1-6. https://doi.org/10.1016/j.rinim.2011.12.001

The aim of this study was to determine the effect of HNSCC tumour treatment on systemic Th1 and Th2 cytokine levels and investigate correlations with clinicopathological parameters. IL2, IL4, IL5, IL6, IL8, IL10, IL13, GMCSF, IFNγ and TNFα were measu... Read More about Effect of treatment on systemic cytokines in head and neck squamous cell carcinoma patients.

Serum IL10, IL12 and circulating CD4+CD25high T regulatory cells in relation to long-term clinical outcome in head and neck squamous cell carcinoma patients (2011)
Journal Article
Green, V. L., Irune, E., Prasai, A., Alhamarneh, O., Greenman, J., & Stafford, N. D. (2012). Serum IL10, IL12 and circulating CD4+CD25high T regulatory cells in relation to long-term clinical outcome in head and neck squamous cell carcinoma patients. International Journal of Oncology, 40(3), 833-839. https://doi.org/10.3892/ijo.2011.1259

IL10, but not IL12 or T regulatory cells in the circulation of newly presenting, pre-treatment head and neck squamous cell carcinoma (HNSCC) patients, has been shown previously to be related to survival over a mean follow-up period of 15 months. Here... Read More about Serum IL10, IL12 and circulating CD4+CD25high T regulatory cells in relation to long-term clinical outcome in head and neck squamous cell carcinoma patients.

On-chip integrated labelling, transport and detection of tumour cells (2011)
Journal Article
Woods, J., Docker, P. T., Dyer, C. E., Haswell, S. J., & Greenman, J. (2011). On-chip integrated labelling, transport and detection of tumour cells. ELECTROPHORESIS, 32(22), 3188-3195. https://doi.org/10.1002/elps.201100172

Microflow cytometry represents a promising tool for the investigation of diagnostic and prognostic cellular cancer markers, particularly if integrated within a device that allows primary cells to be freshly isolated from the solid tumour biopsies tha... Read More about On-chip integrated labelling, transport and detection of tumour cells.

A microfluidic system for testing the responses of head and neck squamous cell carcinoma tissue biopsies to treatment with chemotherapy drugs (2011)
Journal Article
Hattersley, S. M., Sylvester, D. C., Dyer, C. E., Stafford, N. D., Haswell, S. J., & Greenman, J. (2012). A microfluidic system for testing the responses of head and neck squamous cell carcinoma tissue biopsies to treatment with chemotherapy drugs. Annals of biomedical engineering, 40(6), 1277-1288. https://doi.org/10.1007/s10439-011-0428-9

Tumors are heterogeneous masses of cells characterized pathologically by their size and spread. Their chaotic biology makes treatment of malignancies hard to generalize. We present a robust and reproducible glass microfluidic system, for the maintena... Read More about A microfluidic system for testing the responses of head and neck squamous cell carcinoma tissue biopsies to treatment with chemotherapy drugs.

Study of ethanol induced toxicity in liver explants using microfluidic devices. (2011)
Journal Article
Hattersley, S. M., Greenman, J., & Haswell, S. J. (2011). Study of ethanol induced toxicity in liver explants using microfluidic devices. Biomedical Microdevices, 13(6), 1005-1014. https://doi.org/10.1007/s10544-011-9570-2

Current in vitro methodologies for the culture and analysis of liver specific responses lack the sophistication of in vivo dynamics. In this work, a microfluidic based experimental methodology has been utilized to reproduce a biomimetic microenvironm... Read More about Study of ethanol induced toxicity in liver explants using microfluidic devices..

Protein profiling of angiogenesis-related growth factors in laryngeal carcinoma: Pattern of protein expression in relation to tumour progression (2011)
Journal Article
Korampalli, T. S., Green, V. L., Greenman, J., & Stafford, N. D. (2011). Protein profiling of angiogenesis-related growth factors in laryngeal carcinoma: Pattern of protein expression in relation to tumour progression. International Journal of Oncology, 39(4), 1033-1039. https://doi.org/10.3892/ijo.2011.1117

The expression of angiogenesis-related proteins was determined in laryngeal tumour tissue, associated tumour involved lymph nodes, apparently normal mucosa and control tissue and were related to tumour stage. Both laryngeal tumour tissue and associat... Read More about Protein profiling of angiogenesis-related growth factors in laryngeal carcinoma: Pattern of protein expression in relation to tumour progression.