All Outputs (102)

Analysis of radiation-induced cell death in head and neck squamous cell carcinoma and rat liver maintained in microfluidic devices (2013)
Journal Article
Green, V. L., Greenman, J., Carr, S. D., & Stafford, N. D. (2014). Analysis of radiation-induced cell death in head and neck squamous cell carcinoma and rat liver maintained in microfluidic devices. Otolaryngology and head and neck surgery, 150(1), 73-80. https://doi.org/10.1177/0194599813507427

Objective The aim of this study was to investigate how head and neck squamous cell carcinoma (HNSCC) tissue biopsies maintained in a pseudo in vivo environment within a bespoke microfluidic device respond to radiation treatment. Study Design Feasibi... Read More about Analysis of radiation-induced cell death in head and neck squamous cell carcinoma and rat liver maintained in microfluidic devices.

Increased frequency and suppressive activity of CD127low/- regulatory T cells in the peripheral circulation of patients with head and neck squamous cell carcinoma are associated with advanced stage and nodal involvement (2013)
Journal Article
Drennan, S., Stafford, N. D., Greenman, J., & Green, V. (2013). Increased frequency and suppressive activity of CD127low/- regulatory T cells in the peripheral circulation of patients with head and neck squamous cell carcinoma are associated with advanced stage and nodal involvement. Immunology, 140(3), 335-343. https://doi.org/10.1111/imm.12144

The presence of regulatory T (Treg) cells is thought to be an important mechanism by which head and neck squamous cell carcinoma (HNSCC) successfully evades the immune system. Using multicolour flow cytometry, the frequency and functional capacity of... Read More about Increased frequency and suppressive activity of CD127low/- regulatory T cells in the peripheral circulation of patients with head and neck squamous cell carcinoma are associated with advanced stage and nodal involvement.

Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device (2012)
Journal Article
Kemp, C., Birch, C., Shaw, K. J., Nixon, G. J., Docker, P. T., Greenman, J., Huggett, J. F., Haswell, S. J., Foy, C. A., & Dyer, C. E. (2012). Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device. Analytical Methods, 4(7), 2141-2144. https://doi.org/10.1039/c2ay25075f

Urine is a preferred specimen for nucleic acid-based detection of sexually transmitted infections (STIs) but represents a challenge for microfluidic devices due to low analyte concentrations. We present an extraction methodology enabling rapid on-chi... Read More about Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device.

Increased inducible heat shock protein 72 expression associated with PBMC isolated from patients with haematological tumours (2012)
Journal Article
Madden, L. A., Hayman, Y. A., Underwood, C., Vince, R. V., Greenman, J., Allsup, D., & Ali, S. (2012). Increased inducible heat shock protein 72 expression associated with PBMC isolated from patients with haematological tumours. Scandinavian journal of clinical & laboratory investigation, 72(5), 380-386. https://doi.org/10.3109/00365513.2012.681683

Background. Heat shock protein 72 (Hsp72) is a highly inducible stress protein and molecular chaperone. Cancers have been shown to be associated with increased Hsp72 expression within the tumour itself and this may lead to resistance to apoptosis. Me... Read More about Increased inducible heat shock protein 72 expression associated with PBMC isolated from patients with haematological tumours.

Intraluminal thrombus has a selective influence on matrix metalloproteinases and their inhibitors (tissue inhibitors of matrix metalloproteinases) in the wall of abdominal aortic aneurysms (2012)
Journal Article
Abdul Rahman, M., Mazari, F., Khan, J. A., Madden, L., McCollum, P., Shahin, Y., Chetter, I., Greenman, J., Fagan, M., & Smith, G. (2012). Intraluminal thrombus has a selective influence on matrix metalloproteinases and their inhibitors (tissue inhibitors of matrix metalloproteinases) in the wall of abdominal aortic aneurysms. Annals of vascular surgery, 26(3), 322-329. https://doi.org/10.1016/j.avsg.2011.08.015

BACKGROUND The influence of intraluminal thrombus (ILT) on the proteolytic environment within the wall of an abdominal aortic aneurysm (AAA) is unknown. This is the first study to examine the correlation between ILT thickness and the levels of matrix... Read More about Intraluminal thrombus has a selective influence on matrix metalloproteinases and their inhibitors (tissue inhibitors of matrix metalloproteinases) in the wall of abdominal aortic aneurysms.

Evaluation of heart tissue viability under redox-magnetohydrodynamics conditions: Toward fine-tuning flow in biological microfluidics applications (2012)
Journal Article
Cheah, L. T., Fritsch, I., Haswell, S. J., & Greenman, J. (2012). Evaluation of heart tissue viability under redox-magnetohydrodynamics conditions: Toward fine-tuning flow in biological microfluidics applications. Biotechnology and bioengineering, 109(7), 1827-1834. https://doi.org/10.1002/bit.24426

A microfluidic system containing a chamber for heart tissue biopsies, perfused with Krebs–Henseleit buffer containing glucose and antibiotic (KHGB) using peristaltic pumps and continuously stimulated, was used to evaluate tissue viability under redox... Read More about Evaluation of heart tissue viability under redox-magnetohydrodynamics conditions: Toward fine-tuning flow in biological microfluidics applications.

Voltammetric Immunoassay for the Detection of Protein Biomarkers (2012)
Journal Article
Haswell, S. J., Dou, Y.-H., Wadhawan, J., & Greenman, J. (2012). Voltammetric Immunoassay for the Detection of Protein Biomarkers. Electroanalysis, 24(2), 264-272. https://doi.org/10.1002/elan.201100676

A strategy for a fast (ca. 20 min), specific, electrochemical immunoassay for the cardiac biomarker creatine kinase (CK) and the human cytokine interleukin 10 (IL10) has been developed in this paper. The polyaniline modified gold surface formed from... Read More about Voltammetric Immunoassay for the Detection of Protein Biomarkers.

Reply to “Analysis of NO and its metabolites by mass spectrometry. Comment on ‘Detection of nitric oxide in tissue samples by ESI-MS’” (2011)
Journal Article
Zheng, S., Webster, A., Zheng, S., Welham, K. J., Dyer, C. E., Greenman, J., Haswell, S. J., Webster, A., Zheng, S., Welham, K. J., Dyer, C. E., Greenman, J., Haswell, S. J., Zheng, S., Webster, A., Welham, K. J., Dyer, C. E., Greenman, J., Haswell, S. J., Zheng, S., …Haswell, S. J. (2011). Reply to “Analysis of NO and its metabolites by mass spectrometry. Comment on ‘Detection of nitric oxide in tissue samples by ESI-MS’”. Analyst, 136(2), 411-411. https://doi.org/10.1039/c0an00729c

Comment by Tsikas et al. (Analyst, 2011, DOI: 10.1039/c0an00411a) on the preliminary work by Shen and colleagues (Analyst, 2010, 135, 302) describing the use of ESI-MS/MS for the detection of methylpiperazinobenzenediamine, as a probe for NO in tissu... Read More about Reply to “Analysis of NO and its metabolites by mass spectrometry. Comment on ‘Detection of nitric oxide in tissue samples by ESI-MS’”.

Effect of treatment on systemic cytokines in head and neck squamous cell carcinoma patients (2011)
Journal Article
Green, V. L., Michno, A., Greenman, J., & Stafford, N. D. (2012). Effect of treatment on systemic cytokines in head and neck squamous cell carcinoma patients. Results in immunology, 2, 1-6. https://doi.org/10.1016/j.rinim.2011.12.001

The aim of this study was to determine the effect of HNSCC tumour treatment on systemic Th1 and Th2 cytokine levels and investigate correlations with clinicopathological parameters. IL2, IL4, IL5, IL6, IL8, IL10, IL13, GMCSF, IFNγ and TNFα were measu... Read More about Effect of treatment on systemic cytokines in head and neck squamous cell carcinoma patients.

Serum IL10, IL12 and circulating CD4+CD25high T regulatory cells in relation to long-term clinical outcome in head and neck squamous cell carcinoma patients (2011)
Journal Article
Green, V. L., Irune, E., Prasai, A., Alhamarneh, O., Greenman, J., & Stafford, N. D. (2012). Serum IL10, IL12 and circulating CD4+CD25high T regulatory cells in relation to long-term clinical outcome in head and neck squamous cell carcinoma patients. International Journal of Oncology, 40(3), 833-839. https://doi.org/10.3892/ijo.2011.1259

IL10, but not IL12 or T regulatory cells in the circulation of newly presenting, pre-treatment head and neck squamous cell carcinoma (HNSCC) patients, has been shown previously to be related to survival over a mean follow-up period of 15 months. Here... Read More about Serum IL10, IL12 and circulating CD4+CD25high T regulatory cells in relation to long-term clinical outcome in head and neck squamous cell carcinoma patients.

On-chip integrated labelling, transport and detection of tumour cells (2011)
Journal Article
Woods, J., Docker, P. T., Dyer, C. E., Haswell, S. J., & Greenman, J. (2011). On-chip integrated labelling, transport and detection of tumour cells. ELECTROPHORESIS, 32(22), 3188-3195. https://doi.org/10.1002/elps.201100172

Microflow cytometry represents a promising tool for the investigation of diagnostic and prognostic cellular cancer markers, particularly if integrated within a device that allows primary cells to be freshly isolated from the solid tumour biopsies tha... Read More about On-chip integrated labelling, transport and detection of tumour cells.

A microfluidic system for testing the responses of head and neck squamous cell carcinoma tissue biopsies to treatment with chemotherapy drugs (2011)
Journal Article
Hattersley, S. M., Sylvester, D. C., Dyer, C. E., Stafford, N. D., Haswell, S. J., & Greenman, J. (2012). A microfluidic system for testing the responses of head and neck squamous cell carcinoma tissue biopsies to treatment with chemotherapy drugs. Annals of biomedical engineering, 40(6), 1277-1288. https://doi.org/10.1007/s10439-011-0428-9

Tumors are heterogeneous masses of cells characterized pathologically by their size and spread. Their chaotic biology makes treatment of malignancies hard to generalize. We present a robust and reproducible glass microfluidic system, for the maintena... Read More about A microfluidic system for testing the responses of head and neck squamous cell carcinoma tissue biopsies to treatment with chemotherapy drugs.

Study of ethanol induced toxicity in liver explants using microfluidic devices. (2011)
Journal Article
Hattersley, S. M., Greenman, J., & Haswell, S. J. (2011). Study of ethanol induced toxicity in liver explants using microfluidic devices. Biomedical Microdevices, 13(6), 1005-1014. https://doi.org/10.1007/s10544-011-9570-2

Current in vitro methodologies for the culture and analysis of liver specific responses lack the sophistication of in vivo dynamics. In this work, a microfluidic based experimental methodology has been utilized to reproduce a biomimetic microenvironm... Read More about Study of ethanol induced toxicity in liver explants using microfluidic devices..

Protein profiling of angiogenesis-related growth factors in laryngeal carcinoma: Pattern of protein expression in relation to tumour progression (2011)
Journal Article
Korampalli, T. S., Green, V. L., Greenman, J., & Stafford, N. D. (2011). Protein profiling of angiogenesis-related growth factors in laryngeal carcinoma: Pattern of protein expression in relation to tumour progression. International Journal of Oncology, 39(4), 1033-1039. https://doi.org/10.3892/ijo.2011.1117

The expression of angiogenesis-related proteins was determined in laryngeal tumour tissue, associated tumour involved lymph nodes, apparently normal mucosa and control tissue and were related to tumour stage. Both laryngeal tumour tissue and associat... Read More about Protein profiling of angiogenesis-related growth factors in laryngeal carcinoma: Pattern of protein expression in relation to tumour progression.

Microsystems for personalized biomolecular diagnostics (2011)
Journal Article
Shaw, K. J., Birch, C., Hughes, E. M., Jakes, A. D., Greenman, J., & Haswell, S. J. (2011). Microsystems for personalized biomolecular diagnostics. Engineering in Life Sciences, 11(2), 121-132. https://doi.org/10.1002/elsc.201000175

The development of microfluidic methodology that can be used in conjunction with drug screening and biomolecular diagnostics offers a route to evidence-based personalized medical care. Ideally, all personal diagnostics are best carried out in a rapid... Read More about Microsystems for personalized biomolecular diagnostics.

Flow focussing of particles and cells based on their intrinsic properties using a simple diamagnetic repulsion setup (2011)
Journal Article
Rodríguez-Villarreal, A. I., Tarn, M. D., Madden, L. A., Lutz, J. B., Greenman, J., Samitier, J., & Pamme, N. (2011). Flow focussing of particles and cells based on their intrinsic properties using a simple diamagnetic repulsion setup. Lab on a chip, 11(7), 1240-1248. https://doi.org/10.1039/c0lc00464b

The continuous flow focussing and manipulation of particles and cells are important factors in microfluidic applications for performing accurate and reproducible procedures downstream. Many particle focussing methods require complex setups or channel... Read More about Flow focussing of particles and cells based on their intrinsic properties using a simple diamagnetic repulsion setup.

Identification of methicillin-resistant Staphylococcus aureus-specific peptides for targeted photoantimicrobial chemotherapy (2011)
Journal Article
Vince, R. V., Madden, L. A., Alonso, C. M., Savoie, H., Boyle, R. W., Todman, M., Paget, T., & Greenman, J. (2011). Identification of methicillin-resistant Staphylococcus aureus-specific peptides for targeted photoantimicrobial chemotherapy. Photochemical & photobiological sciences : an international journal, 10(4), 515-522. https://doi.org/10.1039/c0pp00267d

The increasing prevalence of multi-drug resistant bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), necessitates development of alternative modes of bacterial targeting which are not hindered by antibiotic resistance and minimise... Read More about Identification of methicillin-resistant Staphylococcus aureus-specific peptides for targeted photoantimicrobial chemotherapy.

Development of a real-world direct interface for integrated DNA extraction and amplification in a microfluidic device (2010)
Journal Article
Shaw, K. J., Joyce, D., Docker, P. T., Dyer, C. E., Greenway, G. M., Greenman, J., & Haswell, S. J. (2011). Development of a real-world direct interface for integrated DNA extraction and amplification in a microfluidic device. Lab on a chip, 11(3), 443-448. https://doi.org/10.1039/c0lc00346h

Integrated DNA extraction and amplification have been carried out in a microfluidic device using electro-osmotic pumping (EOP) for fluidic control. All the necessary reagents for performing both DNA extraction and polymerase chain reaction (PCR) ampl... Read More about Development of a real-world direct interface for integrated DNA extraction and amplification in a microfluidic device.

Thalidomide enhances cyclophosphamide and dexamethasone-mediated cytotoxicity towards cultured chronic lymphocytic leukaemia cells (2010)
Journal Article
Pointon, J. C., Eagle, G., Bailey, J., Evans, P., Allsup, D., & Greenman, J. (2010). Thalidomide enhances cyclophosphamide and dexamethasone-mediated cytotoxicity towards cultured chronic lymphocytic leukaemia cells. Oncology Reports, 24(5), 1315-1321. https://doi.org/10.3892/or_00000988

Numerous chemotherapeutic regimens exist for the treatment of symptomatic or progressive chronic lymphocytic leukaemia (CLL). However, once the disease becomes refractory to nucleoside-based therapy the prognosis is poor. In this study we investigate... Read More about Thalidomide enhances cyclophosphamide and dexamethasone-mediated cytotoxicity towards cultured chronic lymphocytic leukaemia cells.

Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action (2010)
Journal Article
Smith, K., Malatesti, N., Cauchon, N., Hunting, D., Lecomte, R., van Lier, J. E., Greenman, J., & Boyle, R. W. (2011). Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action. Immunology, 132(2), 256-265. https://doi.org/10.1111/j.1365-2567.2010.03359.x

Two cationic porphyrins bearing an isothiocyanate group for conjugation to monocolonal antibodies have been synthesized. The two porphyrins conjugated efficiently to three monoclonal antibodies (anti-CD104, anti-CD146 and anti-CD326), which recognize... Read More about Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.